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Literature summary extracted from

  • Motorin, Y.; Burhenne, J.; Teimer, R.; Koynov, K.; Willnow, S.; Weinhold, E.; Helm, M.
    Expanding the chemical scope of RNA:methyltransferases to site-specific alkynylation of RNA for click labeling (2011), Nucleic Acids Res., 39, 1943-1952.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.215 expression of His6-tagged Trm1 in Escherichia coli strain BL21 (DE3) Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.215 AdoEnYn a double-activated AdoMet analogue containing a pent-2-en-4-ynyl (EnYn) side chain instead of the methyl group. The double bond in beta-position to the sulfonium center counteracts unfavorable steric interactions within the SN2-like transition by conjugative stabilization and the terminal alkyne serves as reactive bio-orthogonal functionality. tRNAPhe methylation reaction to be inhibited by micromolar concentrations of AdoEnYn. EnYn-modified RNA structure determination by mass spectrometry Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.215 4 S-adenosyl-L-methionine + guanine26/guanine27 in tRNA Pyrococcus furiosus
-
 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.215 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.215 recombinant His6-tagged Trm1 from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.215 4 S-adenosyl-L-methionine + guanine26/guanine27 in tRNA
-
 Pyrococcus furiosus 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 in tRNA
-
 ?
2.1.1.215 4 S-adenosyl-L-methionine + guanine26/guanine27 in tRNAPhe tRNAPhe from yeast Pyrococcus furiosus 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 in tRNAPhe
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.215 Trm1
-
 Pyrococcus furiosus
2.1.1.215 tRNA:m22G26-methyltransferase
-
 Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.215 S-adenosyl-L-methionine
-
 Pyrococcus furiosus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.1.1.215 0.1
-
 pH and temperature not specified in the publication Pyrococcus furiosus AdoEnYn