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Literature summary extracted from

  • Beck, K.; Lipps, G.
    Properties of an unusual DNA primase from an archaeal plasmid (2007), Nucleic Acids Res., 35, 5635-5645.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.102 expression in Escherichia coli, wild-type and mutant enzymes Sulfolobus islandicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.102 delC370 deletion mutant retains the strict ATP dependence for primer synthesis Sulfolobus islandicus
2.7.7.102 delC526 deletion mutant retains the strict ATP dependence for primer synthesis Sulfolobus islandicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.102 adenosine 5'-(beta,gamma-imido)triphosphate
-
 Sulfolobus islandicus
2.7.7.102 adenosine 5'-(beta,gamma-methylenetriphosphate)
-
 Sulfolobus islandicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.102 additional information
-
 additional information pH 7.5, 50°C, kinetic properties of the primase activity Sulfolobus islandicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.102 NTP + 7 dNTP Sulfolobus islandicus primases are specialized DNA-dependent RNA polymerases that synthesize a short oligoribonucleotide complementary to single-stranded template DNA. In the context of cellular DNA replication, primases are indispensable since DNA polymerases are not able to start DNA polymerization de novo N(pdN)7 + 7 diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.102 Sulfolobus islandicus Q54324 plasmid pRN1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.102
-
 Sulfolobus islandicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.102 ATP + 7 dNTP enzyme synthesizes short (8 nt-long) primers in the presence of single-stranded M13 DNA. The 12-bases-long substrate with the sequence 5'-CTTCTTCTGTGC-3' represents the minimal substrate which supports synthesis of the full-length primer. The base 3' to the central GTG motif is critical for primer synthesis and that the bases 5' of the GTG determine the length of the primer or run-off product but do not appear to be important for the efficiency of primer synthesis Sulfolobus islandicus A(pdN)7 + 7 diphosphate
-
 ?
2.7.7.102 NTP + 7 dNTP primases are specialized DNA-dependent RNA polymerases that synthesize a short oligoribonucleotide complementary to single-stranded template DNA. In the context of cellular DNA replication, primases are indispensable since DNA polymerases are not able to start DNA polymerization de novo Sulfolobus islandicus N(pdN)7 + 7 diphosphate
-
 ?
2.7.7.102 NTP + 7 dNTP the enzyme synthesizes a mixed primer consisting of a single ribonucleotide at the 5' end followed by seven deoxynucleotides. Ribonucleotides and deoxynucleotides are strictly required at the respective positions within the primer. The primase can initiate primer synthesis with an ATP regardless of the base 5' of the GTG motif and then proceed to synthesize a primer made up of dNTPs. The primase activity is highly sequence-specific and requires the trinucleotide motif GTG in the template. Primer synthesis starts outside of the recognition motif, immediately 5' to the recognition motif Sulfolobus islandicus N(pdN)7 + 7 diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.102 protein ORF904
-
 Sulfolobus islandicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.102 50
-
 assay at Sulfolobus islandicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.102 additional information
-
 additional information pH 7.5, 50°C, kinetic properties of the primase activity Sulfolobus islandicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.102 7.5
-
 assay at Sulfolobus islandicus

General Information

EC Number General Information Comment Organism
2.7.7.102 physiological function primases are specialized DNA-dependent RNA polymerases that synthesize a short oligoribonucleotide complementary to single-stranded template DNA. In the context of cellular DNA replication, primases are indispensable since DNA polymerases are not able to start DNA polymerization de novo Sulfolobus islandicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.102 additional information
-
 additional information pH 7.5, 50°C, kinetic properties of the primase activity Sulfolobus islandicus