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Literature summary extracted from

  • De Falco, M.; Fusco, A.; De Felice, M.; Rossi, M.; Pisani, F.M.
    The DNA primase of Sulfolobus solfataricus is activated by substrates containing a thymine-rich bubble and has a 3'-terminal nucleotidyl-transferase activity (2004), Nucleic Acids Res., 32, 5223-5230.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.B16 additional information synthetic function is specifically activated by thymine-containing synthetic bubble structures that mimic early replication intermediates Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.B16 small and large subunit are co-expressed in Escherichia coli as hexa-Histidine tagged proteins Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.B16 36000
-
1 * 36000 + 1 * 38000, SDS-PAGE Saccharolobus solfataricus
2.7.7.B16 38000
-
1 * 36000 + 1 * 38000, SDS-PAGE Saccharolobus solfataricus
2.7.7.B16 80000
-
gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.B16 dNTP + n dNTP Saccharolobus solfataricus
-
dN(pdN)n + n diphosphate
-
?
2.7.7.B16 dNTP + n dNTP Saccharolobus solfataricus P2
-
dN(pdN)n + n diphosphate
-
?
2.7.7.B16 NTP + n NTP Saccharolobus solfataricus
-
N(pN)n + n diphosphate
-
?
2.7.7.B16 NTP + n NTP Saccharolobus solfataricus P2
-
N(pN)n + n diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.B16 Saccharolobus solfataricus Q9UWW1 and Q97Z83 Q9UWW1: large subunit, Q97Z83: small subunit
-
2.7.7.B16 Saccharolobus solfataricus P2 Q9UWW1 and Q97Z83 Q9UWW1: large subunit, Q97Z83: small subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.B16
-
Saccharolobus solfataricus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.B16 dNTP + n dNTP = dN(pdN)n + n diphosphate (2) Saccharolobus solfataricus
2.7.7.B16 NTP + n NTP = N(pN)n + n diphosphate (1) Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.B16 dNTP + n dNTP
-
Saccharolobus solfataricus dN(pdN)n + n diphosphate
-
?
2.7.7.B16 dNTP + n dNTP utilizes poly-pyrimidine single-stranded DNA templates with low efficiency for de novo synthesis of RNA primers. Its synthetic function is specifically activated by thymine-containing synthetic bubble structures that mimic early replication intermediates. The enzyme is able to incorporate dATP on oligo(A)15/poly(dT) but with lower efficiency with respect to ATP. In addition, the Sso DNA primase is not able to elongate oligo(rA)15 when it is not annealed to poly(dT) or to initiate de novo synthesis on poly(dT) with dATP Saccharolobus solfataricus dN(pdN)n + n diphosphate
-
?
2.7.7.B16 dNTP + n dNTP
-
Saccharolobus solfataricus P2 dN(pdN)n + n diphosphate
-
?
2.7.7.B16 dNTP + n dNTP utilizes poly-pyrimidine single-stranded DNA templates with low efficiency for de novo synthesis of RNA primers. Its synthetic function is specifically activated by thymine-containing synthetic bubble structures that mimic early replication intermediates. The enzyme is able to incorporate dATP on oligo(A)15/poly(dT) but with lower efficiency with respect to ATP. In addition, the Sso DNA primase is not able to elongate oligo(rA)15 when it is not annealed to poly(dT) or to initiate de novo synthesis on poly(dT) with dATP Saccharolobus solfataricus P2 dN(pdN)n + n diphosphate
-
?
2.7.7.B16 additional information the enzyme although shows 3'-terminal nucleotidyl-transferase activity Saccharolobus solfataricus ?
-
?
2.7.7.B16 additional information the enzyme although shows 3'-terminal nucleotidyl-transferase activity Saccharolobus solfataricus P2 ?
-
?
2.7.7.B16 NTP + n NTP
-
Saccharolobus solfataricus N(pN)n + n diphosphate
-
?
2.7.7.B16 NTP + n NTP utilizes poly-pyrimidine single-stranded DNA templates with low efficiency for de novo synthesis of RNA primers. Its synthetic function is specifically activated by thymine-containing synthetic bubble structures that mimic early replication intermediates. The enzyme is able to incorporate dATP on oligo(rA)15/poly(dT) but with lower efficiency with respect to rATP. In addition, the Sso DNA primase is not able to elongate oligo(rA)15 when it is not annealed to poly(dT) or to initiate de novo synthesis on poly(dT) with dATP Saccharolobus solfataricus N(pN)n + n diphosphate
-
?
2.7.7.B16 NTP + n NTP
-
Saccharolobus solfataricus P2 N(pN)n + n diphosphate
-
?
2.7.7.B16 NTP + n NTP utilizes poly-pyrimidine single-stranded DNA templates with low efficiency for de novo synthesis of RNA primers. Its synthetic function is specifically activated by thymine-containing synthetic bubble structures that mimic early replication intermediates. The enzyme is able to incorporate dATP on oligo(rA)15/poly(dT) but with lower efficiency with respect to rATP. In addition, the Sso DNA primase is not able to elongate oligo(rA)15 when it is not annealed to poly(dT) or to initiate de novo synthesis on poly(dT) with dATP Saccharolobus solfataricus P2 N(pN)n + n diphosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.B16 heterodimer 1 * 36000 + 1 * 38000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.B16 60
-
assay at Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.B16 5.5
-
assay at Saccharolobus solfataricus