EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.B16 | additional information | synthetic function is specifically activated by thymine-containing synthetic bubble structures that mimic early replication intermediates | Saccharolobus solfataricus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.B16 | small and large subunit are co-expressed in Escherichia coli as hexa-Histidine tagged proteins | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.B16 | 36000 | - |
1 * 36000 + 1 * 38000, SDS-PAGE | Saccharolobus solfataricus |
2.7.7.B16 | 38000 | - |
1 * 36000 + 1 * 38000, SDS-PAGE | Saccharolobus solfataricus |
2.7.7.B16 | 80000 | - |
gel filtration | Saccharolobus solfataricus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.B16 | dNTP + n dNTP | Saccharolobus solfataricus | - |
dN(pdN)n + n diphosphate | - |
? | |
2.7.7.B16 | dNTP + n dNTP | Saccharolobus solfataricus P2 | - |
dN(pdN)n + n diphosphate | - |
? | |
2.7.7.B16 | NTP + n NTP | Saccharolobus solfataricus | - |
N(pN)n + n diphosphate | - |
? | |
2.7.7.B16 | NTP + n NTP | Saccharolobus solfataricus P2 | - |
N(pN)n + n diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.B16 | Saccharolobus solfataricus | Q9UWW1 and Q97Z83 | Q9UWW1: large subunit, Q97Z83: small subunit | - |
2.7.7.B16 | Saccharolobus solfataricus P2 | Q9UWW1 and Q97Z83 | Q9UWW1: large subunit, Q97Z83: small subunit | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.B16 | - |
Saccharolobus solfataricus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.7.B16 | dNTP + n dNTP = dN(pdN)n + n diphosphate | (2) | Saccharolobus solfataricus | |
2.7.7.B16 | NTP + n NTP = N(pN)n + n diphosphate | (1) | Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.B16 | dNTP + n dNTP | - |
Saccharolobus solfataricus | dN(pdN)n + n diphosphate | - |
? | |
2.7.7.B16 | dNTP + n dNTP | utilizes poly-pyrimidine single-stranded DNA templates with low efficiency for de novo synthesis of RNA primers. Its synthetic function is specifically activated by thymine-containing synthetic bubble structures that mimic early replication intermediates. The enzyme is able to incorporate dATP on oligo(A)15/poly(dT) but with lower efficiency with respect to ATP. In addition, the Sso DNA primase is not able to elongate oligo(rA)15 when it is not annealed to poly(dT) or to initiate de novo synthesis on poly(dT) with dATP | Saccharolobus solfataricus | dN(pdN)n + n diphosphate | - |
? | |
2.7.7.B16 | dNTP + n dNTP | - |
Saccharolobus solfataricus P2 | dN(pdN)n + n diphosphate | - |
? | |
2.7.7.B16 | dNTP + n dNTP | utilizes poly-pyrimidine single-stranded DNA templates with low efficiency for de novo synthesis of RNA primers. Its synthetic function is specifically activated by thymine-containing synthetic bubble structures that mimic early replication intermediates. The enzyme is able to incorporate dATP on oligo(A)15/poly(dT) but with lower efficiency with respect to ATP. In addition, the Sso DNA primase is not able to elongate oligo(rA)15 when it is not annealed to poly(dT) or to initiate de novo synthesis on poly(dT) with dATP | Saccharolobus solfataricus P2 | dN(pdN)n + n diphosphate | - |
? | |
2.7.7.B16 | additional information | the enzyme although shows 3'-terminal nucleotidyl-transferase activity | Saccharolobus solfataricus | ? | - |
? | |
2.7.7.B16 | additional information | the enzyme although shows 3'-terminal nucleotidyl-transferase activity | Saccharolobus solfataricus P2 | ? | - |
? | |
2.7.7.B16 | NTP + n NTP | - |
Saccharolobus solfataricus | N(pN)n + n diphosphate | - |
? | |
2.7.7.B16 | NTP + n NTP | utilizes poly-pyrimidine single-stranded DNA templates with low efficiency for de novo synthesis of RNA primers. Its synthetic function is specifically activated by thymine-containing synthetic bubble structures that mimic early replication intermediates. The enzyme is able to incorporate dATP on oligo(rA)15/poly(dT) but with lower efficiency with respect to rATP. In addition, the Sso DNA primase is not able to elongate oligo(rA)15 when it is not annealed to poly(dT) or to initiate de novo synthesis on poly(dT) with dATP | Saccharolobus solfataricus | N(pN)n + n diphosphate | - |
? | |
2.7.7.B16 | NTP + n NTP | - |
Saccharolobus solfataricus P2 | N(pN)n + n diphosphate | - |
? | |
2.7.7.B16 | NTP + n NTP | utilizes poly-pyrimidine single-stranded DNA templates with low efficiency for de novo synthesis of RNA primers. Its synthetic function is specifically activated by thymine-containing synthetic bubble structures that mimic early replication intermediates. The enzyme is able to incorporate dATP on oligo(rA)15/poly(dT) but with lower efficiency with respect to rATP. In addition, the Sso DNA primase is not able to elongate oligo(rA)15 when it is not annealed to poly(dT) or to initiate de novo synthesis on poly(dT) with dATP | Saccharolobus solfataricus P2 | N(pN)n + n diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.B16 | heterodimer | 1 * 36000 + 1 * 38000, SDS-PAGE | Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.B16 | 60 | - |
assay at | Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.B16 | 5.5 | - |
assay at | Saccharolobus solfataricus |