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Literature summary extracted from

  • Hwang, K.Y.; Baek, K.; Kim, H.-Y.; Cho, Y.
    The crystal structure of flap endonuclease-1 from Methanococcus jannaschii (1998), Nat. Struct. Biol., 5, 707-713.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.99.B1 expression in Escherichia coli Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.99.B1 crystals are grown at 7°C by hanging drop vapor diffusion method, crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 A resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
3.1.99.B1 Methanocaldococcus jannaschii Q58839
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.99.B1
-
Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
3.1.99.B1 FEN-1
-
Methanocaldococcus jannaschii