Literature summary extracted from
Hwang, K.Y.; Baek, K.; Kim, H.-Y.; Cho, Y.
The crystal structure of flap endonuclease-1 from Methanococcus jannaschii (1998), Nat. Struct. Biol., 5, 707-713.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.99.B1 |
expression in Escherichia coli |
Methanocaldococcus jannaschii |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.1.99.B1 |
crystals are grown at 7°C by hanging drop vapor diffusion method, crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 A resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate |
Methanocaldococcus jannaschii |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.99.B1 |
Methanocaldococcus jannaschii |
Q58839 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.99.B1 |
- |
Methanocaldococcus jannaschii |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.99.B1 |
FEN-1 |
- |
Methanocaldococcus jannaschii |