EC Number | Application | Comment | Organism |
---|---|---|---|
2.4.1.257 | synthesis | engineering of a synthetic pathway in Escherichia coli for the production of eukaryotic trimannosyl chitobiose glycans and the transfer of these glycans to specific asparagine residues in target proteins. Glycan biosynthesis is enabled by four eukaryotic glycosyltransferases, including the yeast uridine diphosphate-N-acetylglucosamine transferases Alg13 and Alg14 and the mannosyltransferases Alg1 and Alg2. By including the bacterial oligosaccharyltransferase PglB from Campylobacter jejuni, glycans are successfully transferred to eukaryotic proteins | Saccharomyces cerevisiae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.99.19 | gene pglB, expressio of the enzyme and the modified substrates in Escherichia coli | Campylobacter jejuni |
2.4.99.19 | gene pglB, expressio of the enzyme and the modified substrates in Escherichia coli | Campylobacter lari |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.99.19 | additional information | Campylobacter jejuni | PglBCj can glycosylate residues in both unstructured and structured regions of eukaryotic acceptor proteins in vivo | ? | - |
? | |
2.4.99.19 | tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | Campylobacter jejuni | - |
tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
2.4.99.19 | tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | Campylobacter lari | - |
tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.257 | Saccharomyces cerevisiae | - |
- |
- |
2.4.99.19 | Campylobacter jejuni | - |
gene pglB | - |
2.4.99.19 | Campylobacter lari | - |
gene pglB | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.99.19 | additional information | PglBCj can glycosylate residues in both unstructured and structured regions of eukaryotic acceptor proteins in vivo | Campylobacter jejuni | ? | - |
? | |
2.4.99.19 | additional information | PglBCj is able to transfer Man3GlcNAc2 to extended sites but not to minimal glycosylation sites in engineered or eukaryotic target proteins | Campylobacter jejuni | ? | - |
? | |
2.4.99.19 | additional information | PglBCl is able to transfer Man3GlcNAc2 to extended sites but not to minimal glycosylation sites in engineered or eukaryotic target proteins | Campylobacter lari | ? | - |
? | |
2.4.99.19 | tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | - |
Campylobacter jejuni | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
2.4.99.19 | tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | - |
Campylobacter lari | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
2.4.99.19 | tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | as glycoprotein targets are used Escherichia coli maltose binding protein which is a native periplasmic protein and the anti-beta-galactosidase single-chain antibody fragment called scFv13-R4 that is modified with an N-terminal cotranslational export signal from Escherichia coli DsbA. The proteins are each modified C-terminally with four tandem repeats of the bacterial glycan acceptor motif DQNAT and recombinantly expressed, overview | Campylobacter jejuni | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? | |
2.4.99.19 | tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine | as glycoprotein targets are used Escherichia coli maltose binding protein which is a native periplasmic protein and the anti-beta-galactosidase single-chain antibody fragment called scFv13-R4 that is modified with an N-terminal cotranslational export signal from Escherichia coli DsbA. The proteins are each modified C-terminally with four tandem repeats of the bacterial glycan acceptor motif DQNAT and recombinantly expressed, overview | Campylobacter lari | tritrans,heptacis-undecaprenyl diphosphate + [protein]-L-asparagine-oligosaccharide | a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.99.19 | OTase | - |
Campylobacter jejuni |
2.4.99.19 | OTase | - |
Campylobacter lari |
2.4.99.19 | PglB | - |
Campylobacter jejuni |
2.4.99.19 | PglB | - |
Campylobacter lari |