Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gazanion, E.; Garcia, D.; Silvestre, R.; Gerard, C.; Guichou, J.F.; Labesse, G.; Seveno, M.; Cordeiro-Da-Silva, A.; Ouaissi, A.; Sereno, D.; Vergnes, B.
    The Leishmania nicotinamidase is essential for NAD+ production and parasite proliferation (2011), Mol. Microbiol., 82, 21-38.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.19 expressed in Escherichia coli BL21(DE3) cells Leishmania infantum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.19 sitting-drop-based and sparse-matrix screening , using 0.7 M Na-citrate and 0.1 M HEPES (pH 7.5) Leishmania infantum

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.19 C161A inactive Leishmania infantum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.19 cytosol under physiological conditions, the enzyme displays a diffuse, mainly cytosolic localization Leishmania infantum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.19 22900
-
 x * 22900, calculated from amino acid sequence Leishmania infantum
3.5.1.19 24000
-
 x * 24000, SDS-PAGE Leishmania infantum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.19 nicotinamide + H2O Leishmania infantum
-
 nicotinate + NH3
-
 ?
3.5.1.19 nicotinamide + H2O Leishmania infantum MHOM/MA/67/ITMAP-263
-
 nicotinate + NH3
-
 ?
3.5.1.19 pyrazinamide + H2O Leishmania infantum
-
 pyrazine-2-carboxylic acid + NH3
-
 ?
3.5.1.19 pyrazinamide + H2O Leishmania infantum MHOM/MA/67/ITMAP-263
-
 pyrazine-2-carboxylic acid + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.19 Leishmania infantum
-
-
-
3.5.1.19 Leishmania infantum MHOM/MA/67/ITMAP-263
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.19 Ni-NTA agarose column chromatography Leishmania infantum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.19 promastigote
-
 Leishmania infantum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.19 nicotinamide + H2O
-
 Leishmania infantum nicotinate + NH3
-
 ?
3.5.1.19 nicotinamide + H2O
-
 Leishmania infantum MHOM/MA/67/ITMAP-263 nicotinate + NH3
-
 ?
3.5.1.19 pyrazinamide + H2O
-
 Leishmania infantum pyrazine-2-carboxylic acid + NH3
-
 ?
3.5.1.19 pyrazinamide + H2O
-
 Leishmania infantum MHOM/MA/67/ITMAP-263 pyrazine-2-carboxylic acid + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.19 ? x * 24000, SDS-PAGE Leishmania infantum
3.5.1.19 ? x * 22900, calculated from amino acid sequence Leishmania infantum

Synonyms

EC Number Synonyms Comment Organism
3.5.1.19 Pnc1
-
 Leishmania infantum

General Information

EC Number General Information Comment Organism
3.5.1.19 malfunction the generation of nicotinamidase null mutants leads to a decrease in NAD+ content, associated with a metabolic shutdown-like phenotype with an extensive lag phase of growth. The null mutants are also unable to establish a sustained infection in a mouse model Leishmania infantum
3.5.1.19 physiological function Leishmania nicotinamidase is essential for NAD+ production and parasite proliferation Leishmania infantum