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Literature summary extracted from

  • Rodrigues-Poveda, C.A.; Perez-Moreno, G.; Vidal, A.E.; Urbina, J.A.; Gonzalez-Pacanowska, D.; Ruiz-Perez, L.M.
    Kinetic analyses and inhibition studies reveal novel features in peptide deformylase 1 from Trypanosoma cruzi (2012), Mol. Biochem. Parasitol., 182, 83-87.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.88 1,10-phenanthroline 1-5 mM strongly activates deformylase activity, activity increases 6fold at 1 mM Trypanosoma cruzi
3.5.1.88 EDTA 1-20 mM strongly activates deformylase activity, activity increases up to 70% at 10 mM Trypanosoma cruzi
3.5.1.88 N-formyl-Met-Ala-Ser the activity increases with substrate concentration up to 35 mM Trypanosoma cruzi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.88 expressed in Escherichia coli Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.88 additional information not inhibited by actinonin Trypanosoma cruzi
3.5.1.88 N-formyl-Met-Ala-Ser the enzyme activity of truncated PDF-1DeltaN37 is inhibited by an excess of N-formyl-Met-Ala-Ser substrate Trypanosoma cruzi
3.5.1.88 Ni2+ less than 40% residual activity at 0.1 mM Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.88 22.37
-
 N-formyl-Met-Ala-Ser truncated PDF-1DeltaN37, in 50 mM KH2PO4, pH 7.5, at 37°C Trypanosoma cruzi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.88 Cu2+ the enzyme is strongly activated by Cu2+ with 50% increase of activity at 0.05 mM Trypanosoma cruzi
3.5.1.88 additional information Co2+, Mg2+, Mn2+ do not significantly affect the enzyme activity Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.88 Trypanosoma cruzi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.88 metal-affinity chromatography Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.88 N-formyl-Met-Ala-Ser + H2O
-
 Trypanosoma cruzi formate + Met-Ala-Ser
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.88 ? x * 28000-30000, SDS-PAGE Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
3.5.1.88 PDF-1
-
 Trypanosoma cruzi
3.5.1.88 peptide deformylase 1
-
 Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.88 0.093
-
 N-formyl-Met-Ala-Ser truncated PDF-1DeltaN37, in 50 mM KH2PO4, pH 7.5, at 37°C Trypanosoma cruzi