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Literature summary extracted from

  • Singh, V.; Chandra, D.; Srivastava, B.S.; Srivastava, R.
    Biochemical and transcription analysis of acetohydroxyacid synthase isoforms in Mycobacterium tuberculosis identifies these enzymes as potential targets for drug development (2011), Microbiology, 157, 29-37.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.6 expression in Escherichia coli Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 11.9
-
 pyruvate pH 7.5, 37°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+ Ks value 0.183 mM Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Mycobacterium tuberculosis O06335 catalytic subunit ilvB2
-
2.2.1.6 Mycobacterium tuberculosis O53554 catalytic subunit ilvX
-
2.2.1.6 Mycobacterium tuberculosis P9WG39 catalytic subunit ilvG
-
2.2.1.6 Mycobacterium tuberculosis P9WG41 catalytic subunit ilvB1
-
2.2.1.6 Mycobacterium tuberculosis H37Rv P9WG39 catalytic subunit ilvG
-
2.2.1.6 Mycobacterium tuberculosis H37Rv P9WG41 catalytic subunit ilvB1
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.6 8
-
 pH 7.5, 37°C Mycobacterium tuberculosis
2.2.1.6 11
-
 presence of 20 mM acetate, pH 7.5, 37°C Mycobacterium tuberculosis
2.2.1.6 36
-
 presence of 20 mM acetate, pH 7.5, 37°C Mycobacterium tuberculosis
2.2.1.6 42
-
 pH 7.5, 37°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 2 pyruvate
-
 Mycobacterium tuberculosis 2-acetolactate + CO2
-
 ?
2.2.1.6 2 pyruvate catalytic subunit ilvG shows positive cooperativity towards substrate and cofactors Mycobacterium tuberculosis 2-acetolactate + CO2
-
 ?
2.2.1.6 2 pyruvate catalytic subunit ilvG shows positive cooperativity towards substrate and cofactors Mycobacterium tuberculosis H37Rv 2-acetolactate + CO2
-
 ?
2.2.1.6 2 pyruvate
-
 Mycobacterium tuberculosis H37Rv 2-acetolactate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.2.1.6 ilvB1
-
 Mycobacterium tuberculosis
2.2.1.6 ilvB2
-
 Mycobacterium tuberculosis
2.2.1.6 ilvG
-
 Mycobacterium tuberculosis
2.2.1.6 ilvX
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 thiamine diphosphate Ks value 0.042 mM Mycobacterium tuberculosis

Expression

EC Number Organism Comment Expression
2.2.1.6 Mycobacterium tuberculosis expression level is 0.16 relative to sigA mRNA. Genome sequence of Mycobacterium tuberculosis reveals four genes encoding catalytic subunits, and one regulatory subunit, ilvN. All these genes are expressed in Mycobacterium tuberculosis growing in vitro additional information
2.2.1.6 Mycobacterium tuberculosis expression level is 0.53 relative to sigA mRNA. Genome sequence of Mycobacterium tuberculosis reveals four genes encoding catalytic subunits, and one regulatory subunit, ilvN. All these genes are expressed in Mycobacterium tuberculosis growing in culture additional information
2.2.1.6 Mycobacterium tuberculosis expression level is 0.6 relative to sigA mRNA. Genome sequence of Mycobacterium tuberculosis reveals four genes encoding catalytic subunits, and one regulatory subunit, ilvN. All these genes are expressed in Mycobacterium tuberculosis growing in vitro additional information
2.2.1.6 Mycobacterium tuberculosis expression level is 0.63 relative to sigA mRNA. Genome sequence of Mycobacterium tuberculosis reveals four genes encoding catalytic subunits, and one regulatory subunit, ilvN. All these genes are expressed in Mycobacterium tuberculosis growing in vitro additional information
2.2.1.6 Mycobacterium tuberculosis expression is upregulated in extended stationary phase, ex vivo, and in acid stress and hypoxic environments up