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Literature summary extracted from

  • Read, M.; Mueller, I.B.; Mitchell, S.L.; Sims, P.F.; Hyde, J.E.
    Dynamic subcellular localization of isoforms of the folate pathway enzyme serine hydroxymethyltransferase (SHMT) through the erythrocytic cycle of Plasmodium falciparum (2010), Malar. J., 9, 351.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 genes pfshmt and PF14_ 0534, encoding isozymes PfSHMTc and PfSHMTm, respectively, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged full-length isozymes Plasmodium falciparum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 apicoplast isozyme PfSHMTc in a stage-specific manner, isozyme PfSHMTc localization in this organelle is less restricted than for the mitochondrion and persists from the late trophozoite to the post-mitotic stages Plasmodium falciparum 20011
-
2.1.2.1 cytoplasm isozyme PfSHMTc during all stages Plasmodium falciparum 5737
-
2.1.2.1 mitochondrion isozyme PfSHMTc in a stage-specific manner. Isozyme PfSHMTm showa a distinctly more pronounced mitochondrial location through most of the erythrocytic cycle, presence of a mitochondrial signal sequence Plasmodium falciparum 5739
-
2.1.2.1 additional information patterns of localization of the two isoforms during the parasite erythrocytic cycle, overview. Both PfSHMTc and PfSHMTm are concentrated in the central region of the parasite that becomes the residual body on erythrocyte lysis and merozoite release Plasmodium falciparum
-
-
2.1.2.1 nucleus isozyme PfSHMTc during all stages Plasmodium falciparum 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 49800
-
 x * 49800, recombinant His-tagged PfSHMTc, SDS-PAGE Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Plasmodium falciparum
-
 tetrahydrofolate + L-serine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Plasmodium falciparum
-
 one enzymatically functional SHMT isozyme, PfSHMTc, and one related, apparently inactive isoform, PfSHMTm, encoded by different genes pfshmt and PF14_ 0534, respectively
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.1 merozoite both PfSHMTc and PfSHMTm are concentrated in the central region of the parasite that becomes the residual body on erythrocyte lysis and merozoite release Plasmodium falciparum
-
2.1.2.1 trophozoite early trophozoites lack visible organellar isozyme PfSHMTc, while it occurs during the mid-to-late trophozoite and schizont stages Plasmodium falciparum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Plasmodium falciparum tetrahydrofolate + L-serine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.1 ? x * 49800, recombinant His-tagged PfSHMTc, SDS-PAGE Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
 Plasmodium falciparum
2.1.2.1 SHMT
-
 Plasmodium falciparum

General Information

EC Number General Information Comment Organism
2.1.2.1 additional information both PfSHMTc and PfSHMTm show dynamic, stage-dependent localization among the different compartments of the parasite and sequence analysis suggests they may also reversibly associate with each other, a factor that may be critical to folate cofactor function, given the apparent lack of enzymic activity of PfSHMTm Plasmodium falciparum
2.1.2.1 physiological function the enzyme is essential for the acquisition of one-carbon units for subsequent transfer reactions Plasmodium falciparum