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Literature summary extracted from

  • Yang, X.; Sun, Z.; Ma, R.; Fan, D.; Moradian-Oldak, J.
    Amelogenin nanorods formation during proteolysis by Mmp-20 (2011), J. Struct. Biol., 176, 220-228.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B6 expressed in Escherichia coli XL1-Blue cells Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B6 amelogenin + H2O Sus scrofa most of the secreted substrate is cleaved after Ser148 to generate the 20000 Da amelogenin (Met1-Ser148), which in turn is cleaved after Trp45 to yield the tyrosine-rich amelogenin polypeptide (Met1-Trp45) and the 13000 Da amelogenin (Leu46-Ser148) ?
-
 ?
3.4.24.B6 additional information Sus scrofa recombinant Mmp-20 is self-activated by its incubation in 4-aminophenylmercuric acetate in 10 mM CaCl2 for 24 h ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B6 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.B6 His-TRAP chelating nickel affinity column chromatography Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B6 amelogenin + H2O most of the secreted substrate is cleaved after Ser148 to generate the 20000 Da amelogenin (Met1-Ser148), which in turn is cleaved after Trp45 to yield the tyrosine-rich amelogenin polypeptide (Met1-Trp45) and the 13000 Da amelogenin (Leu46-Ser148) Sus scrofa ?
-
 ?
3.4.24.B6 additional information recombinant Mmp-20 is self-activated by its incubation in 4-aminophenylmercuric acetate in 10 mM CaCl2 for 24 h Sus scrofa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B6 enamelysin
-
 Sus scrofa
3.4.24.B6 MMP-20
-
 Sus scrofa

General Information

EC Number General Information Comment Organism
3.4.24.B6 physiological function following the C-terminal cleavage of amelogenin by Mmp-20, co-assembly with its fragments leads to formation of nanorod structures whose properties eventually dictate the super-structural organization of enamel matrix, controlling the elongated growth of enamel apatite crystals Sus scrofa