EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.104 | expression in Escherichia coli | Thermus thermophilus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.104 | hanging-drop vapor diffusion method | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.104 | 0.00173 | - |
spermidine | pH 9.0, 37°C | Thermus thermophilus | |
2.5.1.104 | 0.57 | - |
norspermidine | pH 9.0, 37°C | Thermus thermophilus | |
2.5.1.104 | 0.77 | - |
agmatine | pH 9.0, 37°C | Thermus thermophilus | |
2.5.1.104 | 1.64 | - |
S-adenosyl 3-(methylthio)propylamine | pH 9.0, 37°C | Thermus thermophilus | |
2.5.1.104 | 38.31 | - |
tris(3-aminopropyl)amine | pH 9.0, 37°C | Thermus thermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.104 | 36006 | - |
4 * 36006, calculated from sequence | Thermus thermophilus |
2.5.1.104 | 130000 | - |
gel filtration | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.104 | Thermus thermophilus | Q5SK28 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.104 | - |
Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.104 | additional information | only faint activities (less than 10% of spermidine) are detected for diaminopropane [NH2(CH2)3NH2], putrescine, spermine, and caldopentamine | Thermus thermophilus | ? | - |
? | |
2.5.1.104 | S-adenosyl 3-(methylthio)propylamine + agmatine | - |
Thermus thermophilus | S-methyl-5'-thioadenosine + N1-aminopropylagmatine | - |
? | |
2.5.1.104 | S-adenosyl 3-(methylthio)propylamine + homospermidine | low activity | Thermus thermophilus | S-methyl-5'-thioadenosine + ? | - |
? | |
2.5.1.104 | S-adenosyl 3-(methylthio)propylamine + N,N'-bis(3-aminopropyl)-1,3-propanediamine | i.e. thermine. Low activity | Thermus thermophilus | S-methyl-5'-thioadenosine + ? | - |
? | |
2.5.1.104 | S-adenosyl 3-(methylthio)propylamine + norspermidine | - |
Thermus thermophilus | S-methyl-5'-thioadenosine + N,N'-bis(3-aminopropyl)-1,3-propanediamine | i.e. thermine | ? | |
2.5.1.104 | S-adenosyl 3-(methylthio)propylamine + spermidine | - |
Thermus thermophilus | S-methyl-5'-thioadenosine + spermine | no formation of thermospermine (i.e. 1,12-diamino-4,8-diazadodecane) | ? | |
2.5.1.104 | S-adenosyl 3-(methylthio)propylamine + tris(3-aminopropyl)amine | i.e. mitsubishine. Low activity. No formation of tetrakis(3-aminopropyl)ammonium | Thermus thermophilus | S-methyl-5'-thioadenosine + N4-aminopropylthermine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.104 | homotetramer | 4 * 36006, calculated from sequence | Thermus thermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.104 | SpeE | - |
Thermus thermophilus |
2.5.1.104 | TAAPT | - |
Thermus thermophilus |
2.5.1.104 | triamine/agmatine aminopropyltransferase | - |
Thermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.104 | 37 | - |
assay at | Thermus thermophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.104 | 0.29 | - |
tris(3-aminopropyl)amine | pH 9.0, 37°C | Thermus thermophilus | |
2.5.1.104 | 0.37 | - |
agmatine | pH 9.0, 37°C | Thermus thermophilus | |
2.5.1.104 | 0.53 | - |
norspermidine | pH 9.0, 37°C | Thermus thermophilus | |
2.5.1.104 | 0.65 | - |
spermidine | pH 9.0, 37°C | Thermus thermophilus | |
2.5.1.104 | 0.73 | - |
S-adenosyl 3-(methylthio)propylamine | pH 9.0, 37°C | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.104 | 8.5 | - |
at 60°C | Thermus thermophilus |
2.5.1.104 | 9 | - |
assay at | Thermus thermophilus |
2.5.1.104 | 9.5 | - |
at 37°C | Thermus thermophilus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.104 | 10 | - |
stable around pH 10.0 at room temperature | Thermus thermophilus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.104 | 7.6 | - |
tris(3-aminopropyl)amine | pH 9.0, 37°C | Thermus thermophilus | |
2.5.1.104 | 375 | - |
spermidine | pH 9.0, 37°C | Thermus thermophilus | |
2.5.1.104 | 450 | - |
S-adenosyl 3-(methylthio)propylamine | pH 9.0, 37°C | Thermus thermophilus | |
2.5.1.104 | 480 | - |
agmatine | pH 9.0, 37°C | Thermus thermophilus | |
2.5.1.104 | 920 | - |
norspermidine | pH 9.0, 37°C | Thermus thermophilus |