Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zuo, Z.; Rodgers, C.J.; Mikheikin, A.L.;, Trakselis, M.A.
    Characterization of a functional DnaG-type primase in archaea: implications for a dual-primase system (2010), J. Mol. Biol., 397, 664-676.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.101 expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.101 E175Q mutation drastically reduces priming activity, low level of DNA binding activity Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.101 0.085
-
 NTP KM-value is determined by measuring the priming rate at different concentrations of NTPs, 70°C, pH 8.5, 1 mM Mn2+, in presence of M13 ssDNA as template, wild-type enzyme Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.101 Mg2+ either Mg2+ or Mn2+ stimulates primer formation over that without any metals or Zn2+. A combination of both Mg2+ and Mn2+ does not stimulate further primer synthesis over that of the single metal Mn2+ Saccharolobus solfataricus
2.7.7.101 Mn2+ either Mg2+ or Mn2+ stimulates primer formation over that without any metals or Zn2+. A combination of both Mg2+ and Mn2+ does not stimulate further primer synthesis over that of the single metal Mn2+ Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.101 NTP + n NTP Saccharolobus solfataricus
-
 N(pN)n + n diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.101 Saccharolobus solfataricus P95980
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.101
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.101 NTP + n NTP
-
 Saccharolobus solfataricus N(pN)n + n diphosphate
-
 ?
2.7.7.101 NTP + n NTP SsoDnaG is only able to utilize NTPs and not dNTPs (in contrast to the heterodimeric eukaryotic DNA primases from archaea are able to incorporate either NTP or dNTP substrates to synthesize RNA, DNA, or hybrid primer products). Slightly positive cooperativity for binding at least two SsoDnaG molecules to DNA. SsoDnaG shows greater than fourfold faster rate of DNA priming over that of eukaryotic-type primase under optimal in vitro conditions. Incorporation of NTPs leads to the formation of primer products that ranged from 2 to 15 nucleotides. Dimer, 4 mer, and 13 mer products are primarily produced Saccharolobus solfataricus N(pN)n + n diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.101 DnaG primase
-
 Saccharolobus solfataricus
2.7.7.101 protein SSO0079
-
 Saccharolobus solfataricus
2.7.7.101 SsoDnaG
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.101 70
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.101 50 90 50°C: about 45% of maximal activity, 90°C: about 35% of maximal activity Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.101 0.00055
-
 NTP KM-value is determined by measuring the priming rate at different concentrations of NTPs, 70°C, pH 8.5, in presence of M13 ssDNA as template, wild-type enzyme Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.101 8
-
 assay at Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.101 0.0000033
-
 NTP KM-value is determined by measuring the priming rate at different concentrations of NTPs, 70°C, pH 8.5, in presence of M13 ssDNA as template, mutant enzyme E175Q Saccharolobus solfataricus
2.7.7.101 0.0063
-
 NTP KM-value is determined by measuring the priming rate at different concentrations of NTPs, 70°C, pH 8.5, 1 mM Mn2+, in presence of M13 ssDNA as template, wild-type enzyme Saccharolobus solfataricus