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Literature summary extracted from

  • Le Breton, M.; Henneke, G.; Norais, C.; Flament, D.; Myllykallio, H.; Querellou, J.; Raffin, J.P.
    The heterodimeric primase from the euryarchaeon Pyrococcus abyssi: a multifunctional enzyme for initiation and repair? (2007), J. Mol. Biol., 374, 1172-1185.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.B16 expression in Escherichia coli Pyrococcus abyssi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.B16 additional information
-
 additional information kinetic parameters for the DNA primase under different metal ion conditions Pyrococcus abyssi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.B16 Mg2+ optimal concentration: 10 mM. Activity is dependent on the presence of Mg2+ and Mn2+. Increased MgCl2 concentrations only slightly enhance NTP incorporation, in contrast to MnCl2 Pyrococcus abyssi
2.7.7.B16 Mn2+ optimal concentration: 5 mM. Activity is dependent on the presence of Mg2+ and Mn2+. Increased MgCl2 concentrations only slightly enhance NTP incorporation, in contrast to MnCl2 Pyrococcus abyssi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.B16 41000
-
 1 * 41000 + 1 * 46000, calculated from sequence Pyrococcus abyssi
2.7.7.B16 46000
-
 1 * 41000 + 1 * 46000, calculated from sequence Pyrococcus abyssi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.B16 NTP + n NTP Pyrococcus abyssi the multifunctional archaeal primase is involved in priming and repair N(pN)n + n diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.B16 Pyrococcus abyssi Q9V292 and Q9V291 Q9V292: small subunit, Q9V291: large subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.B16
-
 Pyrococcus abyssi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.B16 dNTP + n NTP DNA primase has comparable affinities for ribonucleotides and deoxyribonucleotides. The Pabp41 subunit alone has no RNA synthesis activity but could synthesize long (up to 3 kb) DNA strands. Addition of the Pabp46 subunit increases the rate of DNA synthesis but decreases the length of the DNA fragments synthesized and confers RNA synthesis capability. DNA primase also displayed DNA polymerase, gapfilling, and strand-displacement activities Pyrococcus abyssi (dNTP)n+1 + n diphosphate
-
 ?
2.7.7.B16 NTP + n NTP the multifunctional archaeal primase is involved in priming and repair Pyrococcus abyssi N(pN)n + n diphosphate
-
 ?
2.7.7.B16 NTP + n NTP DNA primase has comparable affinities for ribonucleotides and deoxyribonucleotides. The Pabp41 subunit alone has no RNA synthesis activity but could synthesize long (up to 3 kb) DNA strands. Addition of the Pabp46 subunit increases the rate of DNA synthesis but decreases the length of the DNA fragments synthesized and confers RNA synthesis capability. DNA primase also displayed DNA polymerase, gapfilling, and strand-displacement activities Pyrococcus abyssi N(pN)n + n diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.B16 heterodimer 1 * 41000 + 1 * 46000, calculated from sequence Pyrococcus abyssi

Synonyms

EC Number Synonyms Comment Organism
2.7.7.B16 Pabp41
-
 Pyrococcus abyssi
2.7.7.B16 Pabp46
-
 Pyrococcus abyssi

General Information

EC Number General Information Comment Organism
2.7.7.B16 physiological function the heterodimeric primase complex is necessary for archaeal survival Pyrococcus abyssi