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Literature summary extracted from

  • Moreno-Enriquez, A.; Evangelista-Martinez, Z.; Gonzalez-Mondragon, E.G.; Calderon-Flores, A.; Arreguin, R.; Perez-Rueda, E.; Huerta-Saquero, A.
    Biochemical characterization of recombinant L-asparaginase (AnsA) from Rhizobium etli, a member of an increasing rhizobial-type family of L-asparaginases (2012), J. Microbiol. Biotechnol., 22, 292-300.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 gene ansA, phylogenetic analysis, expression of His-tagged AnsA in Escherichia coli strain BL21(DE3) Rhizobium etli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 aspartic acid competitive Rhizobium etli
3.5.1.1 beta-aspartyl hydroxamate 48% inhibition at 5 mM Rhizobium etli
3.5.1.1 Ca2+ 31% inhibition at 1 mM Rhizobium etli
3.5.1.1 D-asparagine 39% inhibition at 5 mM Rhizobium etli
3.5.1.1 Mg2+ 31% inhibition at 1 mM Rhizobium etli
3.5.1.1 Mn2+ 52% inhibition at 1 mM Rhizobium etli
3.5.1.1 additional information poor inhibition by L-glutamine at 5 mM Rhizobium etli
3.5.1.1 NH4+ noncompetitive Rhizobium etli
3.5.1.1 Zn2+ 38% inhibition at 1 mM Rhizobium etli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 8.9
-
 L-asparagine pH 7.5, 37°C, recombinant enzyme Rhizobium etli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 47000
-
 2 * 47000, recombinant His-tagged enzyme, SDS-PAGE Rhizobium etli
3.5.1.1 102000
-
 recombinant His-tagged enzyme, native PAGE Rhizobium etli

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Rhizobium etli Q9RFN5 gene ansA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 recombinant His-tagged AnsA 18fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity Rhizobium etli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 20
-
 purified recombinant enzyme, pH 7.5, 37°C Rhizobium etli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Rhizobium etli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 homodimer 2 * 47000, recombinant His-tagged enzyme, SDS-PAGE Rhizobium etli

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 AnsA
-
 Rhizobium etli
3.5.1.1 L-asparaginase
-
 Rhizobium etli
3.5.1.1 L-asparaginase II
-
 Rhizobium etli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 50
-
-
 Rhizobium etli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.1 30 60 activity range, temperature profile overview Rhizobium etli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.1 50
-
 purified recombinnat enzyme, loss of 60% activity after 20 min, inactivation within 30 min Rhizobium etli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.1 106
-
 L-asparagine pH 7.5, 37°C, recombinant enzyme Rhizobium etli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 9
-
-
 Rhizobium etli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.1 7 11 activity range, pH profile overview Rhizobium etli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.1 10
-
 NH4+ pH 7.5, 37°C, recombinant enzyme Rhizobium etli
3.5.1.1 33
-
 aspartic acid pH 7.5, 37°C, recombinant enzyme Rhizobium etli

General Information

EC Number General Information Comment Organism
3.5.1.1 evolution the enzyme is a member of an increasing rhizobial-type family of L-asparaginases Rhizobium etli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.1 12
-
 L-asparagine pH 7.5, 37°C, recombinant enzyme Rhizobium etli