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Literature summary extracted from

  • Battilana, J.; Emanuelli, F.; Gambino, G.; Gribaudo, I.; Gasperi, F.; Boss, P.K.; Grando, M.S.
    Functional effect of grapevine 1-deoxy-D-xylulose 5-phosphate synthase substitution K284N on Muscat flavour formation (2011), J. Exp. Bot., 62, 5497-5508.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.2.1.7 agriculture single nucleotide polymorphism K284N is significantly associated with Muscat-flavoured varieties. Substitution influences the enzyme kinetics by increasing the catalytic efficiency and also dramatically affects monoterpene levels upon heterologous expression Vitis vinifera

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.7 overexpression in Nicotiana tabacum Vitis vinifera

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.7 K284N single nucleotide polymorphism, significantly associated with Muscat-flavoured varieties. Substitution influences the enzyme kinetics by increasing the catalytic efficiency and also dramatically affects monoterpene levels upon heterologous expression Vitis vinifera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.7 0.585
-
 pyruvate mutant K284N, pH 8.0, 37°C Vitis vinifera
2.2.1.7 0.661
-
 pyruvate wild-type, pH 8.0, 37°C Vitis vinifera
2.2.1.7 1.67
-
 D-glyceraldehyde 3-phosphate mutant K284N, pH 8.0, 37°C Vitis vinifera
2.2.1.7 1.78
-
 D-glyceraldehyde 3-phosphate wild-type, pH 8.0, 37°C Vitis vinifera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.7 77000
-
 x * 77000, SDS-PAGE Vitis vinifera

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.7 Vitis vinifera
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.7 pyruvate + D-glyceraldehyde 3-phosphate
-
 Vitis vinifera 1-deoxy-D-xylulose 5-phosphate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.7 ? x * 77000, SDS-PAGE Vitis vinifera

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.7 37
-
-
 Vitis vinifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.7 8
-
-
 Vitis vinifera

Expression

EC Number Organism Comment Expression
2.2.1.7 Vitis vinifera correlation exists between the transcript profile and the accumulation of free monoterpenol odorants. The modulation of enzyme gene expression during berry development appears to be independent of nucleotide variation in the coding sequence additional information

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.2.1.7 110
-
 pyruvate wild-type, pH 8.0, 37°C Vitis vinifera
2.2.1.7 210
-
 pyruvate mutant K284N, pH 8.0, 37°C Vitis vinifera