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Literature summary extracted from

  • Tang, N.; Wang, Y.; Wang, X.; Zhou, L.; Zhang, F.; Li, X.; Chen, Y.
    Stable overexpression of arginase I and ornithine transcarbamylase in HepG2 cells improves its ammonia detoxification (2012), J. Cell. Biochem., 113, 518-527.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.3.3 biotechnology HepG2 is an immortalized human hepatoma cell line that has been used for research into bioartificial liver systems. However, a low level of ammonia detoxification is its biggest drawback. Stable overexpression of arginase I and ornithine transcarbamylase in HepG2 cells improves its ammonia detoxification Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.3 stable overexpression of arginase I and ornithine transcarbamylase in HepG2 cells using a eukaryotic dual gene expression vector pBudCE4.1, the co-expression improves ammonia detoxification of the cells, improvement of HepG2 cell function, overview Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.3.3 mitochondrion
-
 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine Homo sapiens
-
 L-citrulline + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.3.3 Hep-G2 cell
-
 Homo sapiens
-
2.1.3.3 hepatocyte primary Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Homo sapiens L-citrulline + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.3.3 ornithine transcarbamylase
-
 Homo sapiens
2.1.3.3 OTC
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.3.3 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.3 7.4
-
 assay at Homo sapiens