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Literature summary extracted from

  • Iwamura, A.; Fukami, T.; Higuchi, R.; Nakajima, M.; Yokoi, T.
    Human alpha/beta hydrolase domain containing 10 (ABHD10) is responsible enzyme for deglucuronidation of mycophenolic acid acyl-glucuronide in liver (2012), J. Biol. Chem., 287, 9240-9249.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.93 expressed in Sf9 insect cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.93 5,5'-dithiobis(2-nitrobenzoic acid) the recombinant enzyme is completely inhibited at 1 mM Homo sapiens
3.1.1.93 AgNO3 the recombinant enzyme is completely inhibited at 0.1 mM Homo sapiens
3.1.1.93 bis-4-nitrophenylphosphate the recombinant enzyme shows about 20% residual activity at 1 mM Homo sapiens
3.1.1.93 CdCl2 the recombinant enzyme shows about 20% residual activity at 1 mM Homo sapiens
3.1.1.93 CuCl2 the recombinant enzyme shows about 20% residual activity at 1 mM Homo sapiens
3.1.1.93 diisopropyl fluorophosphate the recombinant enzyme shows about 63% residual activity at 1 mM Homo sapiens
3.1.1.93 Disulfiram the recombinant enzyme shows about 23% residual activity at 1 mM Homo sapiens
3.1.1.93 eserine the recombinant enzyme shows about 85% residual activity at 1 mM Homo sapiens
3.1.1.93 additional information the recombinant enzyme is not inhibited by CoCl2, CaCl2, D-saccharic acid 1,4-lactone, and sodium fluoride Homo sapiens
3.1.1.93 N-ethylmaleimide the recombinant enzyme shows about 75% residual activity at 1 mM Homo sapiens
3.1.1.93 phenylmethylsulfonyl fluoride the recombinant enzyme shows about 25% residual activity at 1 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.93 0.1007
-
 mycophenolic acid O-acyl-glucuronide recombinant enzyme, in 100 mM Tris-HCl (pH 7.4), at 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.93 cytosol
-
 Homo sapiens 5829
-
3.1.1.93 microsome
-
 Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.93 28000
-
 x * 28000, precursor protein, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.93 Homo sapiens Q9NUJ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.93 ammonium sulfate precipitation, CM Sepharose column chromatography, Mono P column chromatography, and Superdex 200 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.93 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.93 0.00017
-
 unpurified enzyme from cytosol, in 100 mM Tris-HCl (pH 7.4), at 37°C Homo sapiens
3.1.1.93 0.00954
-
 after 55.1fold purification, in 100 mM Tris-HCl (pH 7.4), at 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.93 mycophenolic acid O-acyl-glucuronide + H2O
-
 Homo sapiens mycophenolic acid + D-glucuronate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.93 ? x * 28000, precursor protein, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.93 ABHD10
-
 Homo sapiens