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Literature summary extracted from

  • Chemnitz Galal, W.; Pan, M.; Giulian, G.; Yuan, W.; Li, S.; Edwards, J.L.; Marino, J.P.; Kelman, Z.; Hurwitz, J.
    Formation of dAMP-glycerol and dAMP-Tris derivatives by Thermococcus kodakaraensis DNA primase (2012), J. Biol. Chem., 287, 16220-16229.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.7.7.B16 stable to repeated freezing and thawing Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.B16 Thermococcus kodakarensis Q5JJ72 and Q5JJ73 Q5JJ72: small subunit and Q5JJ73: large subunit
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.B16 dATP + glycerol the products are formed by the p41 catalytic subunit alone and the p41-p46 complex in the absence of a DNA template. They are not formed with preparations containing the catalytically inactive p41 subunit Thermococcus kodakarensis dAMP-glycerol + diphosphate
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 ?
2.7.7.B16 dATP + Tris the products are formed by the p41 catalytic subunit alone and the p41-p46 complex in the absence of a DNA template. They are not formed with preparations containing the catalytically inactive p41 subunit Thermococcus kodakarensis dAMP-Tris + diphosphate
-
 ?
2.7.7.B16 dCTP + glycerol the products are formed by the p41 catalytic subunit alone and the p41-p46 complex in the absence of a DNA template. They are not formed with preparations containing the catalytically inactive p41 subunit Thermococcus kodakarensis dAMP-glycerol + diphosphate
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 ?
2.7.7.B16 dCTP + Tris the products are formed by the p41 catalytic subunit alone and the p41-p46 complex in the absence of a DNA template. They are not formed with preparations containing the catalytically inactive p41 subunit Thermococcus kodakarensis dAMP-Tris + diphosphate
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 ?
2.7.7.B16 dGTP + glycerol the products are formed by the p41 catalytic subunit alone and the p41-p46 complex in the absence of a DNA template. They are not formed with preparations containing the catalytically inactive p41 subunit Thermococcus kodakarensis dGMP-glycerol + diphosphate
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 ?
2.7.7.B16 dGTP + Tris the products are formed by the p41 catalytic subunit alone and the p41-p46 complex in the absence of a DNA template. They are not formed with preparations containing the catalytically inactive p41 subunit Thermococcus kodakarensis dGMP-Tris + diphosphate
-
 ?
2.7.7.B16 dTTP + glycerol the products are formed by the p41 catalytic subunit alone and the p41-p46 complex in the absence of a DNA template. They are not formed with preparations containing the catalytically inactive p41 subunit Thermococcus kodakarensis dTMP-glycerol + diphosphate
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 ?
2.7.7.B16 dTTP + Tris the products are formed by the p41 catalytic subunit alone and the p41-p46 complex in the absence of a DNA template. They are not formed with preparations containing the catalytically inactive p41 subunit Thermococcus kodakarensis dTMP-Tris + diphosphate
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 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.B16 70
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 assay at Thermococcus kodakarensis