BRENDA - Enzyme Database show

Alternative spermidine biosynthetic route is critical for growth of Campylobacter jejuni and is the dominant polyamine pathway in human gut microbiota

Hanfrey, C.C.; Pearson, B.M.; Hazeldine, S.; Lee, J.; Gaskin, D.J.; Woster, P.M.; Phillips, M.A.; Michael, A.J.; J. Biol. Chem. 286, 43301-43312 (2011)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.4.1.B4
additional information
deletion of the gene encoding carboxynorspermidine dehydrogenase. The CASDH gene deletion strain grows normally
Campylobacter jejuni
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.4.1.B4
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+
Campylobacter jejuni
-
carboxynorspermidine + NADP+ + H2O
-
-
?
4.1.1.96
additional information
Campylobacter jejuni
the Campylobacter jejuni enzyme is a carboxyspermidine decarboxylase and not a carboxynorspermidine decarboxylase in vivo and is essential for spermidine biosynthesis
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.1.B4
Campylobacter jejuni
A8FJX8
-
-
1.5.1.43
Campylobacter jejuni
-
-
-
4.1.1.96
Campylobacter jejuni
-
NCTC11828
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.1.B4
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+
-
719993
Campylobacter jejuni
carboxynorspermidine + NADP+ + H2O
-
-
-
?
1.5.1.43
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
-
719993
Campylobacter jejuni
carboxyspermidine + H2O + NADP+
-
-
-
?
4.1.1.96
carboxynorspermidine
the enzyme has relatively equal preference for carboxynorspermidine and carboxyspermidine
719993
Campylobacter jejuni
bis(3-aminopropyl)amine + CO2
-
-
-
?
4.1.1.96
carboxyspermidine
the enzyme has relatively equal preference for carboxynorspermidine and carboxyspermidine
719993
Campylobacter jejuni
spermidine + CO2
-
-
-
?
4.1.1.96
additional information
the Campylobacter jejuni enzyme is a carboxyspermidine decarboxylase and not a carboxynorspermidine decarboxylase in vivo and is essential for spermidine biosynthesis
719993
Campylobacter jejuni
?
-
-
-
-
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.4.1.B4
NADPH
-
Campylobacter jejuni
1.5.1.43
NADPH
-
Campylobacter jejuni
4.1.1.96
pyridoxal 5'-phosphate
-
Campylobacter jejuni
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.4.1.B4
NADPH
-
Campylobacter jejuni
1.5.1.43
NADPH
-
Campylobacter jejuni
4.1.1.96
pyridoxal 5'-phosphate
-
Campylobacter jejuni
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.4.1.B4
additional information
deletion of the gene encoding carboxynorspermidine dehydrogenase. The CASDH gene deletion strain grows normally
Campylobacter jejuni
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.4.1.B4
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+
Campylobacter jejuni
-
carboxynorspermidine + NADP+ + H2O
-
-
?
4.1.1.96
additional information
Campylobacter jejuni
the Campylobacter jejuni enzyme is a carboxyspermidine decarboxylase and not a carboxynorspermidine decarboxylase in vivo and is essential for spermidine biosynthesis
?
-
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.1.B4
L-aspartic 4-semialdehyde + 1,3-diaminopropane + NADPH + H+
-
719993
Campylobacter jejuni
carboxynorspermidine + NADP+ + H2O
-
-
-
?
1.5.1.43
L-aspartate 4-semialdehyde + putrescine + NADPH + H+
-
719993
Campylobacter jejuni
carboxyspermidine + H2O + NADP+
-
-
-
?
4.1.1.96
carboxynorspermidine
the enzyme has relatively equal preference for carboxynorspermidine and carboxyspermidine
719993
Campylobacter jejuni
bis(3-aminopropyl)amine + CO2
-
-
-
?
4.1.1.96
carboxyspermidine
the enzyme has relatively equal preference for carboxynorspermidine and carboxyspermidine
719993
Campylobacter jejuni
spermidine + CO2
-
-
-
?
4.1.1.96
additional information
the Campylobacter jejuni enzyme is a carboxyspermidine decarboxylase and not a carboxynorspermidine decarboxylase in vivo and is essential for spermidine biosynthesis
719993
Campylobacter jejuni
?
-
-
-
-
General Information
EC Number
General Information
Commentary
Organism
1.4.1.B4
evolution
the enzyme is found in diverse bacterial phyla, overview. orthologues encoding carboxyspermidine dehydrogenase and carboxyspermidine decarboxylase are found clustered with an array of diverse putrescine biosynthetic genes in different bacterial genomes, consistent with a role in spermidine, rather than sym-norspermidine biosynthesis
Campylobacter jejuni
1.4.1.B4
malfunction
the CASDH gene deletion strain grows normally
Campylobacter jejuni
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.4.1.B4
evolution
the enzyme is found in diverse bacterial phyla, overview. orthologues encoding carboxyspermidine dehydrogenase and carboxyspermidine decarboxylase are found clustered with an array of diverse putrescine biosynthetic genes in different bacterial genomes, consistent with a role in spermidine, rather than sym-norspermidine biosynthesis
Campylobacter jejuni
1.4.1.B4
malfunction
the CASDH gene deletion strain grows normally
Campylobacter jejuni