EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.103 | expressed in Escherichia coli Rosetta II(DE3) pLysS cells | Haemonchus contortus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.103 | amodiaquine | i.e. 4-[(7-chloroquinolin-4-yl)amino]-2-[(diethylamino)methyl]phenol | Haemonchus contortus | |
2.1.1.103 | diphenhydramine | i.e. 2-(diphenylmethoxy)-N,N-dimethylethanamine | Haemonchus contortus | |
2.1.1.103 | miltefosine | i.e. hexadecyl 2-(trimethylammonio)ethyl phosphate | Haemonchus contortus | |
2.1.1.103 | additional information | isozyme PMT1 is not inhibited by diphenhydramine | Haemonchus contortus | |
2.1.1.103 | sinefungin | - |
Haemonchus contortus | |
2.1.1.103 | tacrine | i.e. 1,2,3,4-tetrahydroacridin-9-amine | Haemonchus contortus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.103 | 0.106 | - |
Phosphoethanolamine | isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
2.1.1.103 | 0.217 | - |
phosphodimethylethanolamine | isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
2.1.1.103 | 0.256 | - |
S-adenosyl-L-methionine | isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
2.1.1.103 | 0.277 | - |
S-adenosyl-L-methionine | isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
2.1.1.103 | 0.424 | - |
phosphomonomethylethanolamine | isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.103 | 48000 | - |
1 * 48000, isozyme PMT2, SDS-PAGE | Haemonchus contortus |
2.1.1.103 | 49400 | - |
1 * 49400, isozyme PMT2, calculated from amino acid sequence | Haemonchus contortus |
2.1.1.103 | 51900 | - |
isozyme PMT2, gel filtration | Haemonchus contortus |
2.1.1.103 | 52300 | - |
isozyme PMT1, gel filtration | Haemonchus contortus |
2.1.1.103 | 53100 | - |
1 * 53100, isozyme PMT1, calculated from amino acid sequence | Haemonchus contortus |
2.1.1.103 | 55000 | - |
1 * 55000, isozyme PMT1, SDS-PAGE | Haemonchus contortus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.103 | Haemonchus contortus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.103 | Ni2+-nitrilotriacetic acid-agarose resin column chromatography and Superdex-200 gel filtration | Haemonchus contortus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.103 | 3 S-adenosyl-L-methionine + phosphoethanolamine | overall reaction | Haemonchus contortus | 3 S-adenosyl-L-homocysteine + phosphocholine | - |
? | |
2.1.1.103 | additional information | isozyme PMT2 does not accept phosphoethanolamine as substrate | Haemonchus contortus | ? | - |
? | |
2.1.1.103 | S-adenosyl-L-methionine + phosphodimethylethanolamine | reaction catalyzed by isozyme PMT2 | Haemonchus contortus | S-adenosyl-L-homocysteine + phosphocholine | - |
? | |
2.1.1.103 | S-adenosyl-L-methionine + phosphoethanolamine | reaction catalyzed by isozyme PMT1 | Haemonchus contortus | S-adenosyl-L-homocysteine + phosphomonomethylethanolamine | - |
? | |
2.1.1.103 | S-adenosyl-L-methionine + phosphomonomethylethanolamine | reaction catalyzed by isozyme PMT2 | Haemonchus contortus | S-adenosyl-L-homocysteine + phosphodimethylethanolamine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.103 | monomer | 1 * 48000, isozyme PMT2, SDS-PAGE | Haemonchus contortus |
2.1.1.103 | monomer | 1 * 49400, isozyme PMT2, calculated from amino acid sequence | Haemonchus contortus |
2.1.1.103 | monomer | 1 * 53100, isozyme PMT1, calculated from amino acid sequence | Haemonchus contortus |
2.1.1.103 | monomer | 1 * 55000, isozyme PMT1, SDS-PAGE | Haemonchus contortus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.103 | phosphoethanolamine methyltransferase | - |
Haemonchus contortus |
2.1.1.103 | PMT1 | isozyme PMT1 contains a methyltransferase domain in the N-terminal half of the protein | Haemonchus contortus |
2.1.1.103 | PMT2 | isozyme PMT2 encodes a C-terminal methyltransferase domain | Haemonchus contortus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.103 | 2.28 | - |
S-adenosyl-L-methionine | isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
2.1.1.103 | 4.13 | - |
phosphodimethylethanolamine | isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
2.1.1.103 | 4.9 | - |
phosphomonomethylethanolamine | isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
2.1.1.103 | 5.82 | - |
S-adenosyl-L-methionine | isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
2.1.1.103 | 8.63 | - |
Phosphoethanolamine | isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.1.1.103 | Haemonchus contortus | isozyme PMT1, calculated from amino acid sequence | - |
5.3 |
2.1.1.103 | Haemonchus contortus | isozyme PMT2, calculated from amino acid sequence | - |
5.5 |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.1.1.103 | 0.0125 | - |
isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | miltefosine | |
2.1.1.103 | 0.013 | - |
isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | diphenhydramine | |
2.1.1.103 | 0.0338 | - |
isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | sinefungin | |
2.1.1.103 | 0.0915 | - |
isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | sinefungin | |
2.1.1.103 | 0.1252 | - |
isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | miltefosine | |
2.1.1.103 | 3.1 | - |
isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | amodiaquine | |
2.1.1.103 | 3.4 | - |
isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | amodiaquine | |
2.1.1.103 | 3.5 | - |
isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | tacrine | |
2.1.1.103 | 23.4 | - |
isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | tacrine |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.103 | 8.24 | - |
S-adenosyl-L-methionine | isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
2.1.1.103 | 11.56 | - |
phosphomonomethylethanolamine | isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
2.1.1.103 | 19.05 | - |
phosphodimethylethanolamine | isozyme PMT2, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
2.1.1.103 | 22.72 | - |
S-adenosyl-L-methionine | isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus | |
2.1.1.103 | 81.45 | - |
Phosphoethanolamine | isozyme PMT1, in 0.1 M HEPES (pH 8.0), 2 mM Na2EDTA, 10% glycerol, at 25°C | Haemonchus contortus |