Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kumar, V.P.; West, A.H.; Cook, P.F.
    Kinetic and chemical mechanisms of homocitrate synthase from Thermus thermophilus (2011), J. Biol. Chem., 286, 29428-29439.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.14 expressed in Escherichia coli BL21(DE3) RIL star cells Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.14 CoA in the presence of Mg2+, or Mn2+, product inhibition by CoA is competitive versus acetyl-CoA but uncompetitive versus 2-oxoglutarate Thermus thermophilus
2.3.3.14 EDTA
-
 Thermus thermophilus
2.3.3.14 L-lysine competitive inhibitor versus 2-oxoglutarate up to a concentration of 1 mM Thermus thermophilus
2.3.3.14 additional information not inhibited by oxalate, malonyl-CoA, pantothenate Thermus thermophilus
2.3.3.14 oxalglycine competitive inhibition versus 2-oxoglutarate Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.14 Mg2+ activates Thermus thermophilus
2.3.3.14 Mn2+ activates Thermus thermophilus
2.3.3.14 additional information not activated by 50 mM Zn2+, 5 mM Ni2+ and 5 mM Co2+ Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.14 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.14 Ni-NTA resin column chromatography Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.14 acetyl-CoA + H2O + 2-oxoglutarate
-
 Thermus thermophilus 2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
 ?
2.3.3.14 acetyl-CoA + H2O + oxaloacetate oxaloacetate is a slow alternative substrate Thermus thermophilus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.14 HCS
-
 Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.14 7
-
-
 Thermus thermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.14 0.026
-
 CoA competitive inhibition mode, in 50 mM HEPES, pH 7.5, at 25°C Thermus thermophilus
2.3.3.14 0.11
-
 CoA competitive inhibition mode, in 50 mM HEPES, pH 7.5, at 25°C Thermus thermophilus
2.3.3.14 0.9
-
 oxalglycine in 50 mM HEPES, pH 7.5, at 25°C Thermus thermophilus