Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Huang, K.F.; Liaw, S.S.; Huang, W.L.; Chia, C.Y.; Lo, Y.C.; Chen, Y.L.; Wang, A.H.
    Structures of human Golgi-resident glutaminyl cyclase and its complexes with inhibitors reveal a large loop movement upon inhibitor binding (2011), J. Biol. Chem., 286, 12439-12449.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.5
-
 Homo sapiens
2.3.2.5 expressed in Escherichia coli BL21(DE3) CodonPlus-RIL cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.2.5 mutant enzyme W207F, hanging drop vapor diffusion method, using 1.7 M (NH4)2SO4, 4% (v/v) dioxane, and 100 mM MES, pH 6.5, at 25°C Homo sapiens
2.3.2.5 sitting drop vapor diffusion method, using either 30% (w/v) PEG 200, 5% (w/v) PEG 3000, and 0.1 M MES, pH 6.0, or 50% (w/v) PEG 200, 0.2 M MgCl2, and 0.1 M cacodylate, pH 6.5 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.5 W207F the mutation reduces the turnover rate (kcat) of the enzyme by about 3.7fold compared to the wild type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.5 1-Benzylimidazole
-
 Homo sapiens
2.3.2.5 cacodylate
-
 Homo sapiens
2.3.2.5 Nomega-acetylhistamine
-
 Homo sapiens
2.3.2.5 PBD150
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.5 extracellular
-
 Homo sapiens
-
-
2.3.2.5 Golgi apparatus
-
 Homo sapiens 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.5 Zn2+ the enzyme contains one zinc ion Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.5 Homo sapiens
-
-
-
2.3.2.5 Homo sapiens Q9NXS2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.5
-
 Homo sapiens
2.3.2.5 Ni-NTA column chromatography and Sephacryl S-100 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.5 L-Gln-2-naphthylamide
-
 Homo sapiens 5-oxoprolyl-2-naphthylamide + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.5 glutaminyl cyclase
-
 Homo sapiens
2.3.2.5 Golgi-resident glutaminyl cyclase
-
 Homo sapiens
2.3.2.5 gQC
-
 Homo sapiens
2.3.2.5 sQC secretory glutaminyl cyclase Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.2.5 0.000095
-
 PBD150 secretory glutaminyl cyclase, at 25°C in 50 mM Tris-HCl, pH 8.0 Homo sapiens
2.3.2.5 0.000262
-
 1-Benzylimidazole Golgi-resident glutaminyl cyclase, at 25°C in 50 mM Tris-HCl, pH 8.0 Homo sapiens
2.3.2.5 0.000607
-
 1-Benzylimidazole secretory glutaminyl cyclase, at 25°C in 50 mM Tris-HCl, pH 8.0 Homo sapiens
2.3.2.5 0.001698
-
 Nomega-acetylhistamine secretory glutaminyl cyclase, at 25°C in 50 mM Tris-HCl, pH 8.0 Homo sapiens
2.3.2.5 0.001817
-
 PBD150 Golgi-resident glutaminyl cyclase, at 25°C in 50 mM Tris-HCl, pH 8.0 Homo sapiens
2.3.2.5 0.005748
-
 Nomega-acetylhistamine Golgi-resident glutaminyl cyclase, at 25°C in 50 mM Tris-HCl, pH 8.0 Homo sapiens
2.3.2.5 1.824
-
 cacodylate secretory glutaminyl cyclase, at 25°C in 50 mM Tris-HCl, pH 8.0 Homo sapiens
2.3.2.5 6.696
-
 cacodylate Golgi-resident glutaminyl cyclase, at 25°C in 50 mM Tris-HCl, pH 8.0 Homo sapiens