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Literature summary extracted from

  • Ochi, A.; Makabe, K.; Kuwajima, K.; Hori, H.
    Flexible recognition of the tRNA G18 methylation target site by TrmH methyltransferase through first binding and induced fit processes (2010), J. Biol. Chem., 285, 9018-9029.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.34 expressed in Escherichia coli Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.34 0.000082
-
 guanosine18 in yeast tRNAPhe mutant transcript pH and temperature not specified in the publication, Vmax: 3.1 micromol/mg/h Thermus thermophilus
2.1.1.34 0.0002
-
 guanosine18 in yeast tRNAPhe-GAA mutant transcript pH and temperature not specified in the publication, Vmax: 0.135 micromol/mg/h Thermus thermophilus
2.1.1.34 0.000245
-
 guanosine18 in yeast tRNAPhe-GGA mutant transcript pH and temperature not specified in the publication, Vmax: 1.2 micromol/mg/h Thermus thermophilus
2.1.1.34 0.0003
-
 guanosine18 in yeast tRNAPhe-GUA mutant transcript pH and temperature not specified in the publication, Vmax: 0.18 micromol/mg/h Thermus thermophilus
2.1.1.34 0.00082
-
 guanosine18 in yeast tRNAPhe-dG18 mutant transcript pH and temperature not specified in the publication, Vmax: 0.1 micromol/mg/h Thermus thermophilus
2.1.1.34 0.0125
-
 guanosine18 in yeast tRNAPhe-AGA mutant transcript pH and temperature not specified in the publication, Vmax: 0.00675 micromol/mg/h Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.34 Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.34 additional information yeast tRNAPhe mutant transcripts are prepared in which the modification site and/or other nucleotides in the D-loop are substituted by dG, inosine, or other nucleotides. Sites of methylation are confirmed with RNA sequencing or primer extension. Although the G18G19 sequence is not essential for methylation by TrmH, disruption of G18G19 severely reduces the efficiency of methyl transfer. There is strict recognition of guanosine by TrmH, in that methylation occurs at the adjacent G19 when the G18 is replaced by dG or adenosine. The fact that TrmH methylates guanosine in D-loops from 4 to 12 nucleotides in length suggests that selection of the position of guanosine within the D-loop is relatively flexible Thermus thermophilus ?
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe mutant transcript
-
 Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe mutant transcript
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-AGA mutant transcript
-
 Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-AGA mutant transcript
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-dG18 mutant transcript
-
 Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-dG18 mutant transcript
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-GAA mutant transcript
-
 Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-GAA mutant transcript
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-GGA mutant transcript
-
 Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-GGA mutant transcript
-
 ?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe-GUA mutant transcript
-
 Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe-GUA mutant transcript
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.34 TrmH
-
 Thermus thermophilus