EC Number | Cloned (Comment) | Organism |
---|---|---|
7.4.2.5 | expression in Xenopus laevis oocyte | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
7.4.2.5 | Carbonyl cyanide m-chlorophenylhydrazone | 10 mM, 20% residual activity; 10 mM, 26% residual activity | Arabidopsis thaliana | |
7.4.2.5 | diethyl dicarbonate | 10 mM, 0.4% residual activity; 10 mM, 0.6% residual activity | Arabidopsis thaliana | |
7.4.2.5 | L-Phe-L-Ala | in the absence of dipeptides, transporter shows proton-dependent leak currents that are inhibited by Phe-Ala; in the absence of dipeptides, transporter shows proton-dependent leak currents that are inhibited by Phe-Ala, Trp-Ala, and Phe-Phe | Arabidopsis thaliana | |
7.4.2.5 | L-Phe-L-Phe | in the absence of dipeptides, transporter shows proton-dependent leak currents that are inhibited by Phe-Ala, Trp-Ala, and Phe-Phe. Phe-Ala reduces leak currents by binding to the substrate-binding site with a high apparent affinity | Arabidopsis thaliana | |
7.4.2.5 | L-Trp-L-Ala | in the absence of dipeptides, transporter shows proton-dependent leak currents that are inhibited by Phe-Ala, Trp-Ala, and Phe-Phe | Arabidopsis thaliana | |
7.4.2.5 | N-ethylmaleimide | 10 mM, 12% residual activity; 10 mM, 14% residual activity | Arabidopsis thaliana |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.4.2.5 | Arabidopsis thaliana | Q9LFB8 | isoform PTR5 | - |
7.4.2.5 | Arabidopsis thaliana | Q9M390 | isoform PTR1 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.4.2.5 | ATP + H2O + L-Ala-L-Ala/in | K0.5 value 0.023 mM at -140 mV | Arabidopsis thaliana | ADP + phosphate + L-Ala-L-Ala/out | - |
? | |
7.4.2.5 | ATP + H2O + L-Ala-L-Ala/in | K0.5 value 0.057 mM at -140 mV | Arabidopsis thaliana | ADP + phosphate + L-Ala-L-Ala/out | - |
? | |
7.4.2.5 | ATP + H2O + L-Ala-L-Asp/in | K0.5 value 0.058 mM at -140 mV | Arabidopsis thaliana | ADP + phosphate + L-Ala-L-Asp/out | - |
? | |
7.4.2.5 | ATP + H2O + L-Ala-L-Asp/in | K0.5 value 0.129 mM at -140 mV | Arabidopsis thaliana | ADP + phosphate + L-Ala-L-Asp/out | - |
? | |
7.4.2.5 | ATP + H2O + L-Ala-L-Lys/in | K0.5 value 0.142 mM at -140 mV | Arabidopsis thaliana | ADP + phosphate + L-Ala-L-Lys/out | - |
? | |
7.4.2.5 | ATP + H2O + L-Ala-L-Lys/in | K0.5 value 0.167 mM at -140 mV | Arabidopsis thaliana | ADP + phosphate + L-Ala-L-Lys/out | - |
? | |
7.4.2.5 | additional information | dipeptide transport by AtPTR1 is electrogenic and dependent on protons but not sodium. Transport activity is voltage-dependent, and currents increase supralinearly with more negative membrane potentials and do not saturate. The voltage dependence of the apparent affinities differes between Ala-Ala, Ala-Lys, and Ala-Asp and is not conserved between the two transporters AtPTR1 and AtPTR5. The apparent affinity of AtPTR1 for these dipeptides is pH-dependent and decreases with decreasing proton concentration | Arabidopsis thaliana | ? | - |
? | |
7.4.2.5 | additional information | dipeptide transport by AtPTR5 is electrogenic and dependent on protons but not sodium. Transport activity is voltage-dependent, and currents increase supralinearly with more negative membrane potentials and do not saturate. The voltage dependence of the apparent affinities differes between Ala-Ala, Ala-Lys, and Ala-Asp and is not conserved between the two transporters AtPTR1 and AtPTR5 | Arabidopsis thaliana | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.4.2.5 | PTR1 | - |
Arabidopsis thaliana |