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Literature summary extracted from

  • Stamnaes, J.; Pinkas, D.M.; Fleckenstein, B.; Khosla, C.; Sollid, L.M.
    Redox regulation of transglutaminase 2 activity (2010), J. Biol. Chem., 285, 25402-25409.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.13 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.13 C230A the mutant shows about wild type activity but is less susceptible to oxidation than the wild type enzyme Homo sapiens
2.3.2.13 C277S active-site mutant Homo sapiens
2.3.2.13 C370A the kcat/Km is 33% of the wild type enzyme Homo sapiens
2.3.2.13 C371A the kcat/Km is 5% of the wild type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.13 GSSG reversible inactivation, activity can be restored by treatment with dithiothreitol Homo sapiens
2.3.2.13 N-acetyl-P(6-diazo-5-oxo-L-norleucine)LPF-NH2 active-site inhibitor Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.13 11.2
-
 Nalpha-benzyloxycarbonyl-L-Gln-Gly wild type enzyme, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens
2.3.2.13 11.5
-
 Nalpha-benzyloxycarbonyl-L-Gln-Gly mutant enzyme C230A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens
2.3.2.13 30
-
 Nalpha-benzyloxycarbonyl-L-Gln-Gly Km above 0.03 mM, mutant enzyme C370A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens
2.3.2.13 30
-
 Nalpha-benzyloxycarbonyl-L-Gln-Gly Km above 0.03 mM, mutant enzyme C371A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.13 extracellular
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.13 Ca2+ required for catalytic activity Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Homo sapiens
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.2.13 the enzyme is reversibly inactivated by oxidation Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13 Ni-NTA column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 fibronectin-bound gamma-glutamine + 5-biotinamidopentylamine
-
 Homo sapiens fibronectin N5-biotinamidopentylglutamine + NH3
-
 ?
2.3.2.13 Nalpha-benzyloxycarbonyl-L-Gln-Gly + alkylamine
-
 Homo sapiens ?
-
 ?
2.3.2.13 PQPQLPYPQPQLPY-NH2 + 5-biotinamidopentylamine
-
 Homo sapiens ?
-
 ?
2.3.2.13 QLQPFPQPQLPY + 5-biotinamidopentylamine
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.13 TG2
-
 Homo sapiens
2.3.2.13 transglutaminase 2
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.13 0.17
-
 Nalpha-benzyloxycarbonyl-L-Gln-Gly mutant enzyme C230A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens
2.3.2.13 0.19
-
 Nalpha-benzyloxycarbonyl-L-Gln-Gly wild type enzyme, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.2.13 0.00083
-
 Nalpha-benzyloxycarbonyl-L-Gln-Gly Km above 0.03 mM, mutant enzyme C371A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens
2.3.2.13 0.0055
-
 Nalpha-benzyloxycarbonyl-L-Gln-Gly Km above 0.03 mM, mutant enzyme C370A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens
2.3.2.13 0.015
-
 Nalpha-benzyloxycarbonyl-L-Gln-Gly mutant enzyme C230A, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens
2.3.2.13 0.017
-
 Nalpha-benzyloxycarbonyl-L-Gln-Gly wild type enzyme, in 1 M MOPS, 5 mM EDTA, and 50 mM 2-oxoglutarate (pH 7.2), 100 mM CaCl2, at 37°C Homo sapiens