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Literature summary extracted from

  • Brouns, S.J.; Walther, J.; Snijders, A.P.; van de Werken, H.J.; Willemen, H.L.; Worm, P.; de Vos, M.G.; Andersson, A.; Lundgren, M.; Mazon, H.F.; van den Heuvel, R.H.; Nilsson, P.; Salmon, L.; de Vos, W.M.; Wright, P.C.; Bernander, R.; van der Oost, J.
    Identification of the missing links in prokaryotic pentose oxidation pathways: evidence for enzyme recruitment (2006), J. Biol. Chem., 281, 27378-27388.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.26 NADP+ the enzyme prefers NADP+ over NAD+ Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.122 expression in Escherichia coli Saccharolobus solfataricus
1.1.1.427 expression in Escherichia coli Saccharolobus solfataricus
1.2.1.26 expression in Escherichia coli Saccharolobus solfataricus
4.2.1.5 expression in Escherichia coli Saccharolobus solfataricus
4.2.1.141 expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.427 0.04
-
 NADP+ pH 7.5, 80°C Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.122 Zn2+ contains two zinc ions per monomer Saccharolobus solfataricus
1.1.1.427 Zn2+ contains two zinc ions per monomer Saccharolobus solfataricus
4.2.1.5 Mg2+ the enzyme catalyzes the Mg2+-dependent dehydration reaction of D-arabinonate to 2-keto-3-deoxy-D-arabinonate Saccharolobus solfataricus
4.2.1.141 additional information multiple sequence alignment analysis of the enzyme indicates the presence of a metal binding site consisting of Glu143, Glu145, and Asp164, which may implicate a metal dependent activity Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.122 37291
-
 4 * 37291, calculated from sequence Saccharolobus solfataricus
1.1.1.122 149700
-
 mass spectrometry Saccharolobus solfataricus
1.1.1.427 37291
-
 4 * 37291, calculated from sequence Saccharolobus solfataricus
1.1.1.427 149700
-
 mass spectrometry Saccharolobus solfataricus
1.2.1.26 52290
-
 4 * 52290, calculated from sequence Saccharolobus solfataricus
1.2.1.26 210100
-
 mass spectrometry Saccharolobus solfataricus
4.2.1.5 42437
-
 8 * 42437, calculated from sequence Saccharolobus solfataricus
4.2.1.5 340700
-
 mass spectrometry Saccharolobus solfataricus
4.2.1.141 33143
-
 4 * 33143, calculated from sequence Saccharolobus solfataricus
4.2.1.141 132900
-
 mass spectrometry under reducing conditions Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.427 D-arabinose + NADP+ Saccharolobus solfataricus the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway D-arabinono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.427 D-arabinose + NADP+ Saccharolobus solfataricus P2 the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway D-arabinono-1,4-lactone + NADPH + H+
-
 ?
1.2.1.26 2,5-dioxopentanoate + NADP+ + H2O Saccharolobus solfataricus the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway 2-oxoglutarate + NADPH + 2 H+
-
 ?
1.2.1.26 2,5-dioxopentanoate + NADP+ + H2O Saccharolobus solfataricus P2 the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway 2-oxoglutarate + NADPH + 2 H+
-
 ?
4.2.1.5 D-arabinonate Saccharolobus solfataricus the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway 2-dehydro-3-deoxy-D-arabinonate + H2O
-
 ?
4.2.1.5 D-arabinonate Saccharolobus solfataricus P2 the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway 2-dehydro-3-deoxy-D-arabinonate + H2O
-
 ?
4.2.1.141 2-dehydro-3-deoxy-D-arabinonate Saccharolobus solfataricus the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway 2,5-dioxopentanoate + H2O
-
 ?
4.2.1.141 2-dehydro-3-deoxy-D-arabinonate Saccharolobus solfataricus P2 the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway 2,5-dioxopentanoate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.122 Saccharolobus solfataricus Q97YM2
-
-
1.1.1.122 Saccharolobus solfataricus P2 Q97YM2
-
-
1.1.1.427 Saccharolobus solfataricus Q97YM2
-
-
1.1.1.427 Saccharolobus solfataricus P2 Q97YM2
-
-
1.2.1.26 Saccharolobus solfataricus Q97UA1
-
-
1.2.1.26 Saccharolobus solfataricus P2 Q97UA1
-
-
4.2.1.5 Saccharolobus solfataricus Q97U96
-
-
4.2.1.5 Saccharolobus solfataricus P2 Q97U96
-
-
4.2.1.141 Saccharolobus solfataricus Q97UA0
-
-
4.2.1.141 Saccharolobus solfataricus P2 Q97UA0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.427
-
 Saccharolobus solfataricus
1.2.1.26
-
 Saccharolobus solfataricus
4.2.1.5
-
 Saccharolobus solfataricus
4.2.1.141
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.122 6-deoxy-L-galactose + NAD+ i.e. L-fucose Saccharolobus solfataricus 6-deoxy-L-galactono-1,5-lactone + NADH
-
 ?
1.1.1.122 6-deoxy-L-galactose + NAD+ i.e. L-fucose Saccharolobus solfataricus P2 6-deoxy-L-galactono-1,5-lactone + NADH
-
 ?
1.1.1.427 D-arabinose + NADP+
-
 Saccharolobus solfataricus D-arabinono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.427 D-arabinose + NADP+ the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway Saccharolobus solfataricus D-arabinono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.427 D-arabinose + NADP+
-
 Saccharolobus solfataricus P2 D-arabinono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.427 D-arabinose + NADP+ the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway Saccharolobus solfataricus P2 D-arabinono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.427 D-ribose + NADP+
-
 Saccharolobus solfataricus D-ribonolactone + NADPH
-
 ?
1.1.1.427 D-ribose + NADP+
-
 Saccharolobus solfataricus P2 D-ribonolactone + NADPH
-
 ?
1.1.1.427 L-fucose + NADP+ most active with L-fucose as substrate Saccharolobus solfataricus L-fuconolactone + NADPH + H+
-
 ?
1.1.1.427 L-fucose + NADP+ most active with L-fucose as substrate Saccharolobus solfataricus P2 L-fuconolactone + NADPH + H+
-
 ?
1.1.1.427 L-galactose + NADP+
-
 Saccharolobus solfataricus L-galactonolactone + NADPH
-
 ?
1.2.1.26 2,5-dioxopentanoate + NADP+ + H2O
-
 Saccharolobus solfataricus 2-oxoglutarate + NADPH + 2 H+
-
 ?
1.2.1.26 2,5-dioxopentanoate + NADP+ + H2O the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway Saccharolobus solfataricus 2-oxoglutarate + NADPH + 2 H+
-
 ?
1.2.1.26 2,5-dioxopentanoate + NADP+ + H2O
-
 Saccharolobus solfataricus P2 2-oxoglutarate + NADPH + 2 H+
-
 ?
1.2.1.26 2,5-dioxopentanoate + NADP+ + H2O the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway Saccharolobus solfataricus P2 2-oxoglutarate + NADPH + 2 H+
-
 ?
1.2.1.26 DL-glyceraldehyde + NADP+ + H2O
-
 Saccharolobus solfataricus glycerate + NADPH + H+
-
 ?
1.2.1.26 DL-glyceraldehyde + NADP+ + H2O
-
 Saccharolobus solfataricus P2 glycerate + NADPH + H+
-
 ?
1.2.1.26 glycolaldehyde + NADP+ + H2O
-
 Saccharolobus solfataricus glycolate + NADPH + H+
-
 ?
1.2.1.26 glycolaldehyde + NADP+ + H2O
-
 Saccharolobus solfataricus P2 glycolate + NADPH + H+
-
 ?
4.2.1.5 D-arabinonate
-
 Saccharolobus solfataricus 2-dehydro-3-deoxy-D-arabinonate + H2O
-
 ?
4.2.1.5 D-arabinonate the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway Saccharolobus solfataricus 2-dehydro-3-deoxy-D-arabinonate + H2O
-
 ?
4.2.1.5 D-arabinonate
-
 Saccharolobus solfataricus P2 2-dehydro-3-deoxy-D-arabinonate + H2O
-
 ?
4.2.1.5 D-arabinonate the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway Saccharolobus solfataricus P2 2-dehydro-3-deoxy-D-arabinonate + H2O
-
 ?
4.2.1.5 additional information no activity with D-gluconate up to 20 mM Saccharolobus solfataricus ?
-
 ?
4.2.1.5 additional information no activity with D-gluconate up to 20 mM Saccharolobus solfataricus P2 ?
-
 ?
4.2.1.141 2-dehydro-3-deoxy-D-arabinonate the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway Saccharolobus solfataricus 2,5-dioxopentanoate + H2O
-
 ?
4.2.1.141 2-dehydro-3-deoxy-D-arabinonate the catalytic activity of the protein is investigated by performing indirect enzyme assays using arabinonate dehydratase with D-arabinonate as a substrate. A 50% decrease in the yield of 2-dehydro-3-deoxy-D-arabinonate is observed when both enzymes are co-incubated in the presence of Mg2+. Given the fact that D-arabinonate is converted to 2-oxoglutarate in D-arabinose cell-free extract, this enzyme is anticipated to be responsible for the dehydration of 2-dehydro-3-deoxy-D-arabinonate to the aldehyde 2,5-dioxopentanoate. Due to the unavailability of 2-dehydro-3-deoxy-D-arabinonate, it is not possible to show this in a direct enzyme assay. 2-Oxoglutarate is produced in an indirect assay using arabinonate dehydratase, the putative 2-dehydro-3-deoxy-D-arabinonate dehydratase and the predicted aldehyde dehydrogenase Saccharolobus solfataricus 2,5-dioxopentanoate + H2O
-
 ?
4.2.1.141 2-dehydro-3-deoxy-D-arabinonate the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway Saccharolobus solfataricus P2 2,5-dioxopentanoate + H2O
-
 ?
4.2.1.141 2-dehydro-3-deoxy-D-arabinonate the catalytic activity of the protein is investigated by performing indirect enzyme assays using arabinonate dehydratase with D-arabinonate as a substrate. A 50% decrease in the yield of 2-dehydro-3-deoxy-D-arabinonate is observed when both enzymes are co-incubated in the presence of Mg2+. Given the fact that D-arabinonate is converted to 2-oxoglutarate in D-arabinose cell-free extract, this enzyme is anticipated to be responsible for the dehydration of 2-dehydro-3-deoxy-D-arabinonate to the aldehyde 2,5-dioxopentanoate. Due to the unavailability of 2-dehydro-3-deoxy-D-arabinonate, it is not possible to show this in a direct enzyme assay. 2-Oxoglutarate is produced in an indirect assay using arabinonate dehydratase, the putative 2-dehydro-3-deoxy-D-arabinonate dehydratase and the predicted aldehyde dehydrogenase Saccharolobus solfataricus P2 2,5-dioxopentanoate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.122 tetramer 4 * 37291, calculated from sequence Saccharolobus solfataricus
1.1.1.427 tetramer 4 * 37291, calculated from sequence Saccharolobus solfataricus
1.2.1.26 tetramer 4 * 52290, calculated from sequence Saccharolobus solfataricus
4.2.1.5 octamer 8 * 42437, calculated from sequence Saccharolobus solfataricus
4.2.1.141 tetramer 4 * 33143, calculated from sequence Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.122 Sso1300 locus name Saccharolobus solfataricus
1.1.1.427 Sso1300
-
 Saccharolobus solfataricus
1.2.1.26 DopDH
-
 Saccharolobus solfataricus
1.2.1.26 SSO3117
-
 Saccharolobus solfataricus
4.2.1.5 araD
-
 Saccharolobus solfataricus
4.2.1.5 Sso3124
-
 Saccharolobus solfataricus
4.2.1.141 D-KDA dehydratase
-
 Saccharolobus solfataricus
4.2.1.141 KdaD
-
 Saccharolobus solfataricus
4.2.1.141 Sso3118
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.122 80
-
 assay at Saccharolobus solfataricus
1.1.1.427 80
-
 assay at Saccharolobus solfataricus
1.2.1.26 70
-
 assay at Saccharolobus solfataricus
4.2.1.5 70
-
 assay at Saccharolobus solfataricus
4.2.1.5 85
-
-
 Saccharolobus solfataricus
4.2.1.141 70
-
 assay at Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.427 74 100 more than 50% of maximal activity Saccharolobus solfataricus
4.2.1.5 75 92 more than 50% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.122 85
-
 half-life: 42 min Saccharolobus solfataricus
1.1.1.122 90
-
 half-life: 26 min Saccharolobus solfataricus
1.1.1.427 85
-
 half-life: 42 min Saccharolobus solfataricus
1.1.1.427 90
-
 half-life: 26 min Saccharolobus solfataricus
1.2.1.26 86
-
 half-life time: 18 min Saccharolobus solfataricus
1.2.1.26 90
-
 half-life time: below 10 min Saccharolobus solfataricus
4.2.1.5 86
-
 half-life time: 18 min Saccharolobus solfataricus
4.2.1.5 90
-
 half-life time: below 10 min Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.427 17.7
-
 D-ribose pH 7.5, 80°C Saccharolobus solfataricus
1.1.1.427 17.7
-
 L-galactose pH 7.5, 80°C Saccharolobus solfataricus
1.1.1.427 23.8
-
 D-arabinose pH 7.5, 80°C Saccharolobus solfataricus
1.1.1.427 26.8
-
 L-fucose pH 7.5, 80°C Saccharolobus solfataricus
1.2.1.26 4.8
-
 DL-glyceraldehyde pH 7.5, 70°C Saccharolobus solfataricus
1.2.1.26 5.3
-
 glycolaldehyde pH 7.5, 70°C Saccharolobus solfataricus
1.2.1.26 8.6
-
 2,5-dioxopentanoate pH 7.5, 70°C Saccharolobus solfataricus
4.2.1.5 1.8
-
 D-Arabinonate pH 7.5, 70°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.122 7.5
-
 assay at Saccharolobus solfataricus
1.1.1.427 7.5
-
 assay at Saccharolobus solfataricus
1.1.1.427 8.2
-
-
 Saccharolobus solfataricus
1.2.1.26 7.5
-
 assay at Saccharolobus solfataricus
1.2.1.26 7.8
-
-
 Saccharolobus solfataricus
4.2.1.5 6.7
-
-
 Saccharolobus solfataricus
4.2.1.5 7.5
-
 assay at Saccharolobus solfataricus
4.2.1.141 7.5
-
 assay at Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.427 7.3 9.3 more than 50% of maximal activity Saccharolobus solfataricus
1.2.1.26 6.7 8.2 more than 50% of maximal activity Saccharolobus solfataricus
4.2.1.5 5.2 10.2 more than 50% of maximal activity Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.122 NAD+
-
 Saccharolobus solfataricus
1.1.1.427 NADP+ the Km-value for NAD+ is 30fold higher compared to the Km-value for NADP+ Saccharolobus solfataricus
1.2.1.26 NADP+
-
 Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.141 Saccharolobus solfataricus calculated from sequence
-
 5.9