EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.26 | NADP+ | the enzyme prefers NADP+ over NAD+ | Saccharolobus solfataricus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.122 | expression in Escherichia coli | Saccharolobus solfataricus |
1.1.1.427 | expression in Escherichia coli | Saccharolobus solfataricus |
1.2.1.26 | expression in Escherichia coli | Saccharolobus solfataricus |
4.2.1.5 | expression in Escherichia coli | Saccharolobus solfataricus |
4.2.1.141 | expression in Escherichia coli | Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.427 | 0.04 | - |
NADP+ | pH 7.5, 80°C | Saccharolobus solfataricus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.122 | Zn2+ | contains two zinc ions per monomer | Saccharolobus solfataricus | |
1.1.1.427 | Zn2+ | contains two zinc ions per monomer | Saccharolobus solfataricus | |
4.2.1.5 | Mg2+ | the enzyme catalyzes the Mg2+-dependent dehydration reaction of D-arabinonate to 2-keto-3-deoxy-D-arabinonate | Saccharolobus solfataricus | |
4.2.1.141 | additional information | multiple sequence alignment analysis of the enzyme indicates the presence of a metal binding site consisting of Glu143, Glu145, and Asp164, which may implicate a metal dependent activity | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.122 | 37291 | - |
4 * 37291, calculated from sequence | Saccharolobus solfataricus |
1.1.1.122 | 149700 | - |
mass spectrometry | Saccharolobus solfataricus |
1.1.1.427 | 37291 | - |
4 * 37291, calculated from sequence | Saccharolobus solfataricus |
1.1.1.427 | 149700 | - |
mass spectrometry | Saccharolobus solfataricus |
1.2.1.26 | 52290 | - |
4 * 52290, calculated from sequence | Saccharolobus solfataricus |
1.2.1.26 | 210100 | - |
mass spectrometry | Saccharolobus solfataricus |
4.2.1.5 | 42437 | - |
8 * 42437, calculated from sequence | Saccharolobus solfataricus |
4.2.1.5 | 340700 | - |
mass spectrometry | Saccharolobus solfataricus |
4.2.1.141 | 33143 | - |
4 * 33143, calculated from sequence | Saccharolobus solfataricus |
4.2.1.141 | 132900 | - |
mass spectrometry under reducing conditions | Saccharolobus solfataricus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.427 | D-arabinose + NADP+ | Saccharolobus solfataricus | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | D-arabinono-1,4-lactone + NADPH + H+ | - |
? | |
1.1.1.427 | D-arabinose + NADP+ | Saccharolobus solfataricus P2 | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | D-arabinono-1,4-lactone + NADPH + H+ | - |
? | |
1.2.1.26 | 2,5-dioxopentanoate + NADP+ + H2O | Saccharolobus solfataricus | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | 2-oxoglutarate + NADPH + 2 H+ | - |
? | |
1.2.1.26 | 2,5-dioxopentanoate + NADP+ + H2O | Saccharolobus solfataricus P2 | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | 2-oxoglutarate + NADPH + 2 H+ | - |
? | |
4.2.1.5 | D-arabinonate | Saccharolobus solfataricus | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | 2-dehydro-3-deoxy-D-arabinonate + H2O | - |
? | |
4.2.1.5 | D-arabinonate | Saccharolobus solfataricus P2 | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | 2-dehydro-3-deoxy-D-arabinonate + H2O | - |
? | |
4.2.1.141 | 2-dehydro-3-deoxy-D-arabinonate | Saccharolobus solfataricus | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | 2,5-dioxopentanoate + H2O | - |
? | |
4.2.1.141 | 2-dehydro-3-deoxy-D-arabinonate | Saccharolobus solfataricus P2 | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | 2,5-dioxopentanoate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.122 | Saccharolobus solfataricus | Q97YM2 | - |
- |
1.1.1.122 | Saccharolobus solfataricus P2 | Q97YM2 | - |
- |
1.1.1.427 | Saccharolobus solfataricus | Q97YM2 | - |
- |
1.1.1.427 | Saccharolobus solfataricus P2 | Q97YM2 | - |
- |
1.2.1.26 | Saccharolobus solfataricus | Q97UA1 | - |
- |
1.2.1.26 | Saccharolobus solfataricus P2 | Q97UA1 | - |
- |
4.2.1.5 | Saccharolobus solfataricus | Q97U96 | - |
- |
4.2.1.5 | Saccharolobus solfataricus P2 | Q97U96 | - |
- |
4.2.1.141 | Saccharolobus solfataricus | Q97UA0 | - |
- |
4.2.1.141 | Saccharolobus solfataricus P2 | Q97UA0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.427 | - |
Saccharolobus solfataricus |
1.2.1.26 | - |
Saccharolobus solfataricus |
4.2.1.5 | - |
Saccharolobus solfataricus |
4.2.1.141 | - |
Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.122 | 6-deoxy-L-galactose + NAD+ | i.e. L-fucose | Saccharolobus solfataricus | 6-deoxy-L-galactono-1,5-lactone + NADH | - |
? | |
1.1.1.122 | 6-deoxy-L-galactose + NAD+ | i.e. L-fucose | Saccharolobus solfataricus P2 | 6-deoxy-L-galactono-1,5-lactone + NADH | - |
? | |
1.1.1.427 | D-arabinose + NADP+ | - |
Saccharolobus solfataricus | D-arabinono-1,4-lactone + NADPH + H+ | - |
? | |
1.1.1.427 | D-arabinose + NADP+ | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | Saccharolobus solfataricus | D-arabinono-1,4-lactone + NADPH + H+ | - |
? | |
1.1.1.427 | D-arabinose + NADP+ | - |
Saccharolobus solfataricus P2 | D-arabinono-1,4-lactone + NADPH + H+ | - |
? | |
1.1.1.427 | D-arabinose + NADP+ | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | Saccharolobus solfataricus P2 | D-arabinono-1,4-lactone + NADPH + H+ | - |
? | |
1.1.1.427 | D-ribose + NADP+ | - |
Saccharolobus solfataricus | D-ribonolactone + NADPH | - |
? | |
1.1.1.427 | D-ribose + NADP+ | - |
Saccharolobus solfataricus P2 | D-ribonolactone + NADPH | - |
? | |
1.1.1.427 | L-fucose + NADP+ | most active with L-fucose as substrate | Saccharolobus solfataricus | L-fuconolactone + NADPH + H+ | - |
? | |
1.1.1.427 | L-fucose + NADP+ | most active with L-fucose as substrate | Saccharolobus solfataricus P2 | L-fuconolactone + NADPH + H+ | - |
? | |
1.1.1.427 | L-galactose + NADP+ | - |
Saccharolobus solfataricus | L-galactonolactone + NADPH | - |
? | |
1.2.1.26 | 2,5-dioxopentanoate + NADP+ + H2O | - |
Saccharolobus solfataricus | 2-oxoglutarate + NADPH + 2 H+ | - |
? | |
1.2.1.26 | 2,5-dioxopentanoate + NADP+ + H2O | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | Saccharolobus solfataricus | 2-oxoglutarate + NADPH + 2 H+ | - |
? | |
1.2.1.26 | 2,5-dioxopentanoate + NADP+ + H2O | - |
Saccharolobus solfataricus P2 | 2-oxoglutarate + NADPH + 2 H+ | - |
? | |
1.2.1.26 | 2,5-dioxopentanoate + NADP+ + H2O | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | Saccharolobus solfataricus P2 | 2-oxoglutarate + NADPH + 2 H+ | - |
? | |
1.2.1.26 | DL-glyceraldehyde + NADP+ + H2O | - |
Saccharolobus solfataricus | glycerate + NADPH + H+ | - |
? | |
1.2.1.26 | DL-glyceraldehyde + NADP+ + H2O | - |
Saccharolobus solfataricus P2 | glycerate + NADPH + H+ | - |
? | |
1.2.1.26 | glycolaldehyde + NADP+ + H2O | - |
Saccharolobus solfataricus | glycolate + NADPH + H+ | - |
? | |
1.2.1.26 | glycolaldehyde + NADP+ + H2O | - |
Saccharolobus solfataricus P2 | glycolate + NADPH + H+ | - |
? | |
4.2.1.5 | D-arabinonate | - |
Saccharolobus solfataricus | 2-dehydro-3-deoxy-D-arabinonate + H2O | - |
? | |
4.2.1.5 | D-arabinonate | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | Saccharolobus solfataricus | 2-dehydro-3-deoxy-D-arabinonate + H2O | - |
? | |
4.2.1.5 | D-arabinonate | - |
Saccharolobus solfataricus P2 | 2-dehydro-3-deoxy-D-arabinonate + H2O | - |
? | |
4.2.1.5 | D-arabinonate | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | Saccharolobus solfataricus P2 | 2-dehydro-3-deoxy-D-arabinonate + H2O | - |
? | |
4.2.1.5 | additional information | no activity with D-gluconate up to 20 mM | Saccharolobus solfataricus | ? | - |
? | |
4.2.1.5 | additional information | no activity with D-gluconate up to 20 mM | Saccharolobus solfataricus P2 | ? | - |
? | |
4.2.1.141 | 2-dehydro-3-deoxy-D-arabinonate | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | Saccharolobus solfataricus | 2,5-dioxopentanoate + H2O | - |
? | |
4.2.1.141 | 2-dehydro-3-deoxy-D-arabinonate | the catalytic activity of the protein is investigated by performing indirect enzyme assays using arabinonate dehydratase with D-arabinonate as a substrate. A 50% decrease in the yield of 2-dehydro-3-deoxy-D-arabinonate is observed when both enzymes are co-incubated in the presence of Mg2+. Given the fact that D-arabinonate is converted to 2-oxoglutarate in D-arabinose cell-free extract, this enzyme is anticipated to be responsible for the dehydration of 2-dehydro-3-deoxy-D-arabinonate to the aldehyde 2,5-dioxopentanoate. Due to the unavailability of 2-dehydro-3-deoxy-D-arabinonate, it is not possible to show this in a direct enzyme assay. 2-Oxoglutarate is produced in an indirect assay using arabinonate dehydratase, the putative 2-dehydro-3-deoxy-D-arabinonate dehydratase and the predicted aldehyde dehydrogenase | Saccharolobus solfataricus | 2,5-dioxopentanoate + H2O | - |
? | |
4.2.1.141 | 2-dehydro-3-deoxy-D-arabinonate | the enzyme is involved in the conversion of D-arabinose into the tricarboxylic acid cycle intermediate 2-oxoglutarate via the pentose oxidation pathway | Saccharolobus solfataricus P2 | 2,5-dioxopentanoate + H2O | - |
? | |
4.2.1.141 | 2-dehydro-3-deoxy-D-arabinonate | the catalytic activity of the protein is investigated by performing indirect enzyme assays using arabinonate dehydratase with D-arabinonate as a substrate. A 50% decrease in the yield of 2-dehydro-3-deoxy-D-arabinonate is observed when both enzymes are co-incubated in the presence of Mg2+. Given the fact that D-arabinonate is converted to 2-oxoglutarate in D-arabinose cell-free extract, this enzyme is anticipated to be responsible for the dehydration of 2-dehydro-3-deoxy-D-arabinonate to the aldehyde 2,5-dioxopentanoate. Due to the unavailability of 2-dehydro-3-deoxy-D-arabinonate, it is not possible to show this in a direct enzyme assay. 2-Oxoglutarate is produced in an indirect assay using arabinonate dehydratase, the putative 2-dehydro-3-deoxy-D-arabinonate dehydratase and the predicted aldehyde dehydrogenase | Saccharolobus solfataricus P2 | 2,5-dioxopentanoate + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.122 | tetramer | 4 * 37291, calculated from sequence | Saccharolobus solfataricus |
1.1.1.427 | tetramer | 4 * 37291, calculated from sequence | Saccharolobus solfataricus |
1.2.1.26 | tetramer | 4 * 52290, calculated from sequence | Saccharolobus solfataricus |
4.2.1.5 | octamer | 8 * 42437, calculated from sequence | Saccharolobus solfataricus |
4.2.1.141 | tetramer | 4 * 33143, calculated from sequence | Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.122 | Sso1300 | locus name | Saccharolobus solfataricus |
1.1.1.427 | Sso1300 | - |
Saccharolobus solfataricus |
1.2.1.26 | DopDH | - |
Saccharolobus solfataricus |
1.2.1.26 | SSO3117 | - |
Saccharolobus solfataricus |
4.2.1.5 | araD | - |
Saccharolobus solfataricus |
4.2.1.5 | Sso3124 | - |
Saccharolobus solfataricus |
4.2.1.141 | D-KDA dehydratase | - |
Saccharolobus solfataricus |
4.2.1.141 | KdaD | - |
Saccharolobus solfataricus |
4.2.1.141 | Sso3118 | - |
Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.122 | 80 | - |
assay at | Saccharolobus solfataricus |
1.1.1.427 | 80 | - |
assay at | Saccharolobus solfataricus |
1.2.1.26 | 70 | - |
assay at | Saccharolobus solfataricus |
4.2.1.5 | 70 | - |
assay at | Saccharolobus solfataricus |
4.2.1.5 | 85 | - |
- |
Saccharolobus solfataricus |
4.2.1.141 | 70 | - |
assay at | Saccharolobus solfataricus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.427 | 74 | 100 | more than 50% of maximal activity | Saccharolobus solfataricus |
4.2.1.5 | 75 | 92 | more than 50% of maximal activity | Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.122 | 85 | - |
half-life: 42 min | Saccharolobus solfataricus |
1.1.1.122 | 90 | - |
half-life: 26 min | Saccharolobus solfataricus |
1.1.1.427 | 85 | - |
half-life: 42 min | Saccharolobus solfataricus |
1.1.1.427 | 90 | - |
half-life: 26 min | Saccharolobus solfataricus |
1.2.1.26 | 86 | - |
half-life time: 18 min | Saccharolobus solfataricus |
1.2.1.26 | 90 | - |
half-life time: below 10 min | Saccharolobus solfataricus |
4.2.1.5 | 86 | - |
half-life time: 18 min | Saccharolobus solfataricus |
4.2.1.5 | 90 | - |
half-life time: below 10 min | Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.427 | 17.7 | - |
D-ribose | pH 7.5, 80°C | Saccharolobus solfataricus | |
1.1.1.427 | 17.7 | - |
L-galactose | pH 7.5, 80°C | Saccharolobus solfataricus | |
1.1.1.427 | 23.8 | - |
D-arabinose | pH 7.5, 80°C | Saccharolobus solfataricus | |
1.1.1.427 | 26.8 | - |
L-fucose | pH 7.5, 80°C | Saccharolobus solfataricus | |
1.2.1.26 | 4.8 | - |
DL-glyceraldehyde | pH 7.5, 70°C | Saccharolobus solfataricus | |
1.2.1.26 | 5.3 | - |
glycolaldehyde | pH 7.5, 70°C | Saccharolobus solfataricus | |
1.2.1.26 | 8.6 | - |
2,5-dioxopentanoate | pH 7.5, 70°C | Saccharolobus solfataricus | |
4.2.1.5 | 1.8 | - |
D-Arabinonate | pH 7.5, 70°C | Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.122 | 7.5 | - |
assay at | Saccharolobus solfataricus |
1.1.1.427 | 7.5 | - |
assay at | Saccharolobus solfataricus |
1.1.1.427 | 8.2 | - |
- |
Saccharolobus solfataricus |
1.2.1.26 | 7.5 | - |
assay at | Saccharolobus solfataricus |
1.2.1.26 | 7.8 | - |
- |
Saccharolobus solfataricus |
4.2.1.5 | 6.7 | - |
- |
Saccharolobus solfataricus |
4.2.1.5 | 7.5 | - |
assay at | Saccharolobus solfataricus |
4.2.1.141 | 7.5 | - |
assay at | Saccharolobus solfataricus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.427 | 7.3 | 9.3 | more than 50% of maximal activity | Saccharolobus solfataricus |
1.2.1.26 | 6.7 | 8.2 | more than 50% of maximal activity | Saccharolobus solfataricus |
4.2.1.5 | 5.2 | 10.2 | more than 50% of maximal activity | Saccharolobus solfataricus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.122 | NAD+ | - |
Saccharolobus solfataricus | |
1.1.1.427 | NADP+ | the Km-value for NAD+ is 30fold higher compared to the Km-value for NADP+ | Saccharolobus solfataricus | |
1.2.1.26 | NADP+ | - |
Saccharolobus solfataricus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
4.2.1.141 | Saccharolobus solfataricus | calculated from sequence | - |
5.9 |