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Literature summary extracted from

  • Cramer, J.; Restle, T.
    Pre-steady-state kinetic characterization of the DinB homologue DNA polymerase of Sulfolobus solfataricus (2005), J. Biol. Chem., 280, 40552-40558.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Saccharolobus solfataricus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 dATP + DNAn Dbh is a distributive enzyme showing a low DNA and nucleotide binding affinity along with a slow polymerization rate. DNA binding occurs in a single step, diffusion-controlled manner. The rate-limiting step of nucleotide incorporation (correct and incorrect) is the chemical step (phosphoryl transfer) and not a conformational change of the enzyme. An induced fit mechanism to select and incorporate nucleotides during DNA polymerization can not be detected for the enzyme Saccharolobus solfataricus ?
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 ?
2.7.7.7 dCTP + DNAn Dbh is a distributive enzyme showing a low DNA and nucleotide binding affinity along with a slow polymerization rate. DNA binding occurs in a single step, diffusion-controlled manner. The rate-limiting step of nucleotide incorporation (correct and incorrect) is the chemical step (phosphoryl transfer) and not a conformational change of the enzyme. An induced fit mechanism to select and incorporate nucleotides during DNA polymerization can not be detected for the enzyme Saccharolobus solfataricus ?
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 ?
2.7.7.7 dGTP + DNAn Dbh is a distributive enzyme showing a low DNA and nucleotide binding affinity along with a slow polymerization rate. DNA binding occurs in a single step, diffusion-controlled manner. The rate-limiting step of nucleotide incorporation (correct and incorrect) is the chemical step (phosphoryl transfer) and not a conformational change of the enzyme. An induced fit mechanism to select and incorporate nucleotides during DNA polymerization can not be detected for the enzyme Saccharolobus solfataricus ?
-
 ?
2.7.7.7 dTTP + DNAn Dbh is a distributive enzyme showing a low DNA and nucleotide binding affinity along with a slow polymerization rate. DNA binding occurs in a single step, diffusion-controlled manner. The rate-limiting step of nucleotide incorporation (correct and incorrect) is the chemical step (phosphoryl transfer) and not a conformational change of the enzyme. An induced fit mechanism to select and incorporate nucleotides during DNA polymerization can not be detected for the enzyme Saccharolobus solfataricus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 DBH
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 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.7 25
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 assay at Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.7 0.64
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 dTTP pH 7.5, 25°C Saccharolobus solfataricus
2.7.7.7 2.5
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 dATP pH 7.5, 25°C Saccharolobus solfataricus
2.7.7.7 3.7
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 dGTP pH 7.5, 25°C Saccharolobus solfataricus
2.7.7.7 5.6
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 dCTP pH 7.5, 25°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 7.5
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 assay at Saccharolobus solfataricus