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Literature summary extracted from

  • Kennelly, P.J.; Oxenrider, K.A.; Leng, J.; Cantwell, J.S.; Zhao, N.
    Identification of a serine/threonine-specific protein phosphatase from the archaebacterium Sulfolobus solfataricus (1993), J. Biol. Chem., 268, 6505-6510.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.16 Cu2+
-
 Saccharolobus solfataricus
3.1.3.16 diphosphate 10 mM, 93% inhibition Saccharolobus solfataricus
3.1.3.16 EDTA 10 mM, 93% inhibition Saccharolobus solfataricus
3.1.3.16 NaF 50 mM, 78% inhibition Saccharolobus solfataricus
3.1.3.16 phosphate 10 mM, 76% inhibition Saccharolobus solfataricus
3.1.3.16 ZnSO4 1 mM, 91% inhibition Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.16 Co2+ stimulates Saccharolobus solfataricus
3.1.3.16 Mg2+ stimulates Saccharolobus solfataricus
3.1.3.16 Mn2+ stimulates Saccharolobus solfataricus
3.1.3.16 additional information Ca2+, Cu2+, or Zn2+ have little or no stimulatory effect Saccharolobus solfataricus
3.1.3.16 Ni2+ stimulates Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.16 28000
-
 gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.16 Saccharolobus solfataricus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.16 additional information
-
 typical specific activities observed in the presence of Mn2+ are on the order of 40-120 pmol of phosphate released from casein per min per mg of soluble extract protein Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.16 a phosphoprotein + H2O the enzyme is specific from serinel/threonine-phosphorylated proteins including casein, histone H2a, phosphorylase kinase, or glycogen phosphorylase. The enzyme does not display detectable protein phosphatase activity toward a number of tyrosine-phosphorylated substrates Saccharolobus solfataricus a protein + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.16 protein phosphatase
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.16 80
-
 the enzyme is stable to heating for several min at temperatures up to 80°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.16 6.5
-
 casein as substrate Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.16 4.5
-
 virtually no protein phosphatase activity can be detected at pH values below 4.5 Saccharolobus solfataricus