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Literature summary extracted from

  • Hiwatashi, A.; Ichikawa, Y.
    Purification and properties of the tightly bound reduced nicotinamide adenine dinucleotide phosphate-adrenodoxin reductase of bovine adrenocortical mitochondria (1982), J. Biochem., 92, 335-342.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.18.1.6 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.6 51000
-
 x * 51000, tightly bound enzyme form, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.6 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.6 the tightly bound enzyme form is purified by sodium cholate solubilization, ammonium sulfate precipitation, DEAE-Sepharose column chromatography, 2’,5’-ADP-Sepharose column chromatography, and DEAE-Sepharose column chromatography. The loosely bound enzyme form is purified by ammonium sulfate precipitation, DEAE-cellulose column chromatography, 2’,5’-ADP-Sepharose column chromatography, and Sephadex G-25 gel filtration Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.18.1.6 adrenal cortex
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.1.6 0.03
-
 tightly bound enzyme form from homogenate, at pH 7.4 and 25°C Bos taurus
1.18.1.6 22
-
 tightly bound enzyme form after 733fold purification, at pH 7.4 and 25°C Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.18.1.6 -80°C, 50 mm potassium phosphate buffer (pH 7.4) in the dark, at least 3 months, no loss of activity Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.6 oxidized adrenodoxin + NADPH + H+ tightly and loosely bound enzyme forms show similar activities Bos taurus reduced adrenodoxin + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.1.6 ? x * 51000, tightly bound enzyme form, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
1.18.1.6 adrenodoxin reductase
-
 Bos taurus
1.18.1.6 NADPH-adrenodoxin reductase
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.6 FAD
-
 Bos taurus