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Literature summary extracted from

  • Schurig-Briccio, L.A.; Gennis, R.B.
    Characterization of the PIB-type ATPases present in Thermus thermophilus (2012), J. Bacteriol., 194, 4107-4113.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.2.12 expressed in Escherichia coli C43 (DE3) cells Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.2.12 Cd2+ the putative Zn2+/Cd2+-ATPase is truncated at the N-terminus and is activated in vitro by copper but not by zinc or cadmium. When expressed in Escherichia coli, however, the putative enzyme can be isolated as a full-length protein and the ATPase activity is increased by the addition of Zn2+ and Cd2+ as well as by Cu2+ Thermus thermophilus
7.2.2.12 Cu2+ the putative Zn2+/Cd2+-ATPase is truncated at the N-terminus and is activated in vitro by copper but not by zinc or cadmium. When expressed in Escherichia coli, however, the putative enzyme can be isolated as a full-length protein and the ATPase activity is increased by the addition of Zn2+ and Cd2+ as well as by Cu2+ Thermus thermophilus
7.2.2.12 Zn2+ the putative Zn2+/Cd2+-ATPase is truncated at the N-terminus and is activated in vitro by copper but not by zinc or cadmium. When expressed in Escherichia coli, however, the putative enzyme can be isolated as a full-length protein and the ATPase activity is increased by the addition of Zn2+ and Cd2+ as well as by Cu2+ Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.12 Thermus thermophilus
-
-
-
7.2.2.12 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.2.12 Talon resin column chromatography Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.12 ATP + H2O + Zn2+/in
-
 Thermus thermophilus ADP + phosphate + Zn2+/out
-
 ?
7.2.2.12 ATP + H2O + Zn2+/in
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 ADP + phosphate + Zn2+/out
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.2.2.12 zinc-cadmium transporter
-
 Thermus thermophilus
7.2.2.12 Zn2+/Cd2+-ATPase
-
 Thermus thermophilus