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Literature summary extracted from

  • Asencion Diez, M.D.; Peiru, S.; Demonte, A.M.; Gramajo, H.; Iglesias, A.A.
    Characterization of recombinant UDP- and ADP-glucose pyrophosphorylases and glycogen synthase to elucidate glucose-1-phosphate partitioning into oligo- and polysaccharides in Streptomyces coelicolor (2012), J. Bacteriol., 194, 1485-1493.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 D-fructose 6-phosphate 2 mM, 21fold activation Streptomyces coelicolor
2.7.7.27 D-glucose 6-phosphate 2 mM, 33fold activation Streptomyces coelicolor
2.7.7.27 D-mannose 6-phosphate 2 mM, 12fold activation Streptomyces coelicolor
2.7.7.27 D-ribose 5-phosphate 2 mM, 7fold activation Streptomyces coelicolor
2.7.7.27 phosphoenolpyruvate 2 mM, 14fold activation Streptomyces coelicolor
2.7.7.27 pyruvate 2 mM, 3fold activation Streptomyces coelicolor

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.21 expression of N-terminally His-tagged GSase in Escherichia coli strain BL21(DE3) or Top10 Streptomyces coelicolor
2.7.7.27 expression in Escherichia coli Streptomyces coelicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.21 Co2+ over 90% inhibition Streptomyces coelicolor
2.4.1.21 Cu2+ over 90% inhibition Streptomyces coelicolor
2.4.1.21 Ni2+ over 90% inhibition Streptomyces coelicolor
2.4.1.21 Zn2+ over 90% inhibition Streptomyces coelicolor
2.7.7.27 NADPH
-
 Streptomyces coelicolor
2.7.7.27 phosphate
-
 Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.21 additional information
-
 additional information kinetics, overview Streptomyces coelicolor
2.7.7.27 0.35
-
 diphosphate pH 8.0, 37°C Streptomyces coelicolor
2.7.7.27 0.37
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C Streptomyces coelicolor
2.7.7.27 0.39
-
 ATP pH 8.0, 37°C Streptomyces coelicolor
2.7.7.27 2.1
-
 ADP-glucose pH 8.0, 37°C Streptomyces coelicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.21 Mg2+ 1.5fold activation at 10 mM Streptomyces coelicolor
2.4.1.21 Mn2+ 10 mM, 47% of the activity with Mg2+ Streptomyces coelicolor
2.7.7.27 Mg2+ required. Km value in both directions of catalysis about 1.8 mM Streptomyces coelicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.27 42700
-
 4 * 42700, calculated and SDS-PAGE Streptomyces coelicolor
2.7.7.27 180000
-
 gel filtration Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.21 ADP-glucose + [(1->4)-alpha-D-glucosyl]n Streptomyces coelicolor glycogen synthase exhibits specificity in the use of ADP-glucose to elongate alpha-1,4-glucan chains in the polysaccharide ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.21 ADP-glucose + [(1->4)-alpha-D-glucosyl]n Streptomyces coelicolor A3(2) glycogen synthase exhibits specificity in the use of ADP-glucose to elongate alpha-1,4-glucan chains in the polysaccharide ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.21 Streptomyces coelicolor
-
-
-
2.4.1.21 Streptomyces coelicolor A3(2)
-
-
-
2.7.7.27 Streptomyces coelicolor P72394
-
-
2.7.7.27 Streptomyces coelicolor A3(2) P72394
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.21 recombinant N-terminally His-tagged GSase from Escherichia coli strain BL21(DE3) or Top10 by nickel affinity chromatography and dialysis Streptomyces coelicolor

Storage Stability

EC Number Storage Stability Organism
2.4.1.21 -80°C, purified His-tagged enzyme, in 20 mM triethanolamine-HCl, pH 8.0, and 20% v/v glycerol, stable for at least 6 months Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.21 ADP-glucose + [(1->4)-alpha-D-glucosyl]n glycogen synthase exhibits specificity in the use of ADP-glucose to elongate alpha-1,4-glucan chains in the polysaccharide Streptomyces coelicolor ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.21 ADP-glucose + [(1->4)-alpha-D-glucosyl]n substrate is rabbit liver glycogen Streptomyces coelicolor ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.21 ADP-glucose + [(1->4)-alpha-D-glucosyl]n glycogen synthase exhibits specificity in the use of ADP-glucose to elongate alpha-1,4-glucan chains in the polysaccharide Streptomyces coelicolor A3(2) ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.21 ADP-glucose + [(1->4)-alpha-D-glucosyl]n substrate is rabbit liver glycogen Streptomyces coelicolor A3(2) ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate enzyme is specific for ATP and reaches similar activities in the directions of ADP-glucose synthesis or diphosphorolysis Streptomyces coelicolor diphosphate + ADP-glucose
-
 r
2.7.7.27 ATP + alpha-D-glucose 1-phosphate enzyme is specific for ATP and reaches similar activities in the directions of ADP-glucose synthesis or diphosphorolysis Streptomyces coelicolor A3(2) diphosphate + ADP-glucose
-
 r
2.7.7.27 diphosphate + ADP-glucose enzyme reaches similar activities in the directions of ADP-glucose synthesis or diphosphorolysis Streptomyces coelicolor ATP + alpha-D-glucose 1-phosphate
-
 r
2.7.7.27 diphosphate + ADP-glucose enzyme reaches similar activities in the directions of ADP-glucose synthesis or diphosphorolysis Streptomyces coelicolor A3(2) ATP + alpha-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.21 dimer SDS-PAGE and gel filtration, quaternary structure determination, recombinant enzyme Streptomyces coelicolor
2.7.7.27 tetramer 4 * 42700, calculated and SDS-PAGE Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
2.4.1.21 GSase
-
 Streptomyces coelicolor
2.7.7.27 GlgC
-
 Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.21 8
-
 assay at Streptomyces coelicolor

General Information

EC Number General Information Comment Organism
2.4.1.21 metabolism glycogen synthesis occurs via ADP-glucose in Streptomyces coelicolor, with the pathway being strictly regulated in connection with other routes involved with oligo- and polysaccharides, as well as with antibiotic synthesis in the bacterium, metabolic scenario for glucose 6-phosphate partitioning, overview Streptomyces coelicolor