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Literature summary extracted from

  • Bevers, L.E.; Schwarz, G.; Hagen, W.R.
    A molecular basis for tungstate selectivity in prokaryotic ABC transport systems (2011), J. Bacteriol., 193, 4999-5001.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.3.2.6 expression in TOP10 cell Escherichia coli
7.3.2.6 expression in TOP10 cell Pyrococcus furiosus

Protein Variants

EC Number Protein Variants Comment Organism
7.3.2.6 D160A mutant is able to bind both molybdate and tungstate but with lower affinities than wild-type WtpA. Mutant is no longer able to selectively bind tungstate Pyrococcus furiosus
7.3.2.6 D160N mutant is able to bind both molybdate and tungstate but with lower affinities than wild-type WtpA. Mutant is no longer able to selectively bind tungstate Pyrococcus furiosus
7.3.2.6 E218A mutant is able to bind both molybdate and tungstate but with lower affinities than wild-type WtpA. Mutant retains specificity for tungstate and displays presence of two binding sites with different affinities Pyrococcus furiosus
7.3.2.6 E218Q mutant is able to bind both molybdate and tungstate but with lower affinities than wild-type WtpA. Mutant retains specificity for tungstate Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
7.3.2.6 Escherichia coli
-
-
-
7.3.2.6 Pyrococcus furiosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.3.2.6 additional information ModA is able to bind both tungstate and molybdate with very high affinities but is unable to replace one oxoanion with the other in a displacement titration Escherichia coli ?
-
 ?
7.3.2.6 additional information the octahedral W(VI)/Mo(VI)-binding motif present in WtpA proteins is essential for its displayed binding preference for tungstate over molybdate Pyrococcus furiosus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.3.2.6 ModA
-
 Escherichia coli
7.3.2.6 WtpA
-
 Pyrococcus furiosus