EC Number | Cloned (Comment) | Organism |
---|---|---|
3.7.1.19 | expressed in Escherichia coli XL-1 Blue cells | Paenarthrobacter nicotinovorans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.7.1.19 | 0.006 | - |
2,6-dihydroxypseudooxynicotine | in 75 mM phosphate buffer, pH 7.5, temperature not specified in the publication | Paenarthrobacter nicotinovorans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.7.1.19 | 43000 | - |
gel filtration | Paenarthrobacter nicotinovorans |
3.7.1.19 | 43555 | - |
1 * 43555, calculated from amino acid sequence | Paenarthrobacter nicotinovorans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.7.1.19 | Paenarthrobacter nicotinovorans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.7.1.19 | Ni-chelating Sepharose column chromatography | Paenarthrobacter nicotinovorans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.7.1.19 | 2,6-dihydroxypseudooxynicotine + H2O | - |
Paenarthrobacter nicotinovorans | 2,6-dihydroxypyridine + 4-methylaminobutanoate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.7.1.19 | monomer | 1 * 43000, SDS-PAGE | Paenarthrobacter nicotinovorans |
3.7.1.19 | monomer | 1 * 43555, calculated from amino acid sequence | Paenarthrobacter nicotinovorans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.7.1.19 | 2,6-dihydroxypseudooxynicotine hydrolase | - |
Paenarthrobacter nicotinovorans |
3.7.1.19 | DHPONH | - |
Paenarthrobacter nicotinovorans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.7.1.19 | 792 | - |
2,6-dihydroxypseudooxynicotine | in 75 mM phosphate buffer, pH 7.5, temperature not specified in the publication | Paenarthrobacter nicotinovorans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.7.1.19 | 7.5 | - |
- |
Paenarthrobacter nicotinovorans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.7.1.19 | 5 | 10 | - |
Paenarthrobacter nicotinovorans |