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Literature summary extracted from

  • Zhu, D.; Seo, M.J.; Ikeda, H.; Cane, D.E.
    Genome mining in Streptomyces. Discovery of an unprecedented P450-catalyzed oxidative rearrangement that is the final step in the biosynthesis of pentalenolactone (2011), J. Am. Chem. Soc., 133, 2128-2131.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.8 expressed in Escherichia coli BL21(DE3) cells Streptomyces exfoliatus
1.14.19.8 expressed in Escherichia coli BL21(DE3) cells Streptomyces arenae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.19.8 0.34
-
 pentalenolactone F at 30°C, pH not specified in the publication Streptomyces exfoliatus
1.14.19.8 0.43
-
 pentalenolactone F at 30°C, pH not specified in the publication Streptomyces arenae

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.8 Streptomyces arenae
-
-
-
1.14.19.8 Streptomyces arenae TUE469
-
-
-
1.14.19.8 Streptomyces exfoliatus
-
-
-
1.14.19.8 Streptomyces exfoliatus UC5319
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.8 Ni2+-NTA resin column chromatography Streptomyces exfoliatus
1.14.19.8 Ni2+-NTA resin column chromatography Streptomyces arenae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.8 pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+
-
 Streptomyces exfoliatus pentalenolactone + 2 oxidized ferredoxin + 2 H2O
-
 ir
1.14.19.8 pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+
-
 Streptomyces arenae pentalenolactone + 2 oxidized ferredoxin + 2 H2O
-
 ir
1.14.19.8 pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+
-
 Streptomyces exfoliatus UC5319 pentalenolactone + 2 oxidized ferredoxin + 2 H2O
-
 ir
1.14.19.8 pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+
-
 Streptomyces arenae TUE469 pentalenolactone + 2 oxidized ferredoxin + 2 H2O
-
 ir

Subunits

EC Number Subunits Comment Organism
1.14.19.8 monomer
-
 Streptomyces exfoliatus
1.14.19.8 monomer
-
 Streptomyces arenae

Synonyms

EC Number Synonyms Comment Organism
1.14.19.8 penM
-
 Streptomyces exfoliatus
1.14.19.8 pntM
-
 Streptomyces arenae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.19.8 8.8
-
 pentalenolactone F at 30°C, pH not specified in the publication Streptomyces arenae
1.14.19.8 10.5
-
 pentalenolactone F at 30°C, pH not specified in the publication Streptomyces exfoliatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.8 NADPH required for activity Streptomyces exfoliatus
1.14.19.8 NADPH required for activity Streptomyces arenae