EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.15.1.1 | - |
Sulfolobus acidocaldarius |
EC Number | General Stability | Organism |
---|---|---|
1.15.1.1 | treatment with the proteases V8 shows no effect on the enzyme | Sulfolobus acidocaldarius |
1.15.1.1 | treatment with trypsin shows little effect on the enzyme | Sulfolobus acidocaldarius |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.15.1.1 | cytosol | it comprises at least 11% of the cytosolic protein | Sulfolobus acidocaldarius | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.15.1.1 | Iron | contains one iron atom per dimer, the protein contains a mononuclear iron center | Sulfolobus acidocaldarius |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | 22400 | - |
2 * 22400, calculated from sequence | Sulfolobus acidocaldarius |
1.15.1.1 | 22400 | - |
2 * 22400, simultaneous treatment with low SDS concentrations and heat results in an almost quantitative conversion into a tetrameric form of the enzyme with a specific activity of about 2.5 times higher than the activity of the dimer, suggesting that even though the protein is stable and active in a dimeric state, its physiological form may be tetrameric | Sulfolobus acidocaldarius |
1.15.1.1 | 45000 | - |
dimeric form, gel filtration | Sulfolobus acidocaldarius |
1.15.1.1 | 87000 | - |
tetrameric form, gel filtration | Sulfolobus acidocaldarius |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.15.1.1 | guanidine-HCl | after 72 h incubation with 6 M guanidinium hydrochloride at 2O°C, 67% of its enzymatic activity is retaine | Sulfolobus acidocaldarius |
1.15.1.1 | SDS | incubation with 0.1% SDS shows no effect on the enzyme. Simultaneous treatment with low SDS concentrations and heat results in an almost quantitative conversion into a second form of the enzyme with a molecular mass of 87000 Da. It is most likely a tetramer. The specific activity of the tetrameric form is about 2.5 times higher than the activity of the dimer, suggesting that even though the protein is stable and active in a dimeric state, its physiological form may be tetrameric | Sulfolobus acidocaldarius |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.15.1.1 | Sulfolobus acidocaldarius | Q08713 | - |
- |
1.15.1.1 | Sulfolobus acidocaldarius DSM 639 | Q08713 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.15.1.1 | - |
Sulfolobus acidocaldarius |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.15.1.1 | 2 O2.- + 2 H+ | - |
Sulfolobus acidocaldarius | O2 + H2O2 | - |
? | |
1.15.1.1 | 2 O2.- + 2 H+ | - |
Sulfolobus acidocaldarius DSM 639 | O2 + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.15.1.1 | dimer | 2 * 22400, calculated from sequence | Sulfolobus acidocaldarius |
1.15.1.1 | tetramer | 2 * 22400, simultaneous treatment with low SDS concentrations and heat results in an almost quantitative conversion into a tetrameric form of the enzyme with a specific activity of about 2.5 times higher than the activity of the dimer, suggesting that even though the protein is stable and active in a dimeric state, its physiological form may be tetrameric | Sulfolobus acidocaldarius |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.15.1.1 | Fe-superoxide dismutase | - |
Sulfolobus acidocaldarius |