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Literature summary extracted from

  • Chiribau, C.; Mihasan, M.; Ganas, P.; Igloi, G.; Artenie, V.; Brandsch, R.
    Final steps in the catabolism of nicotine (2006), FEBS J., 273, 1528-1536.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.21 2.5
-
 4-methylaminobutanoate pH 9.8, 22°C Paenarthrobacter nicotinovorans
1.5.3.21 6.66
-
 4-aminobutanoate pH 9.8, 22°C Paenarthrobacter nicotinovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.21 46000
-
 x * 46100, calculated, x * 46000, SDS-PAGE Paenarthrobacter nicotinovorans
1.5.3.21 46100
-
 x * 46100, calculated, x * 46000, SDS-PAGE Paenarthrobacter nicotinovorans

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.19 Paenarthrobacter nicotinovorans
-
-
-
1.5.3.21 Paenarthrobacter nicotinovorans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.21 4-aminobutanoate + O2 + H2O
-
 Paenarthrobacter nicotinovorans succinate semialdehyde + NH3 + H2O2
-
 ?
1.5.3.21 4-methylaminobutanoate + O2 + H2O
-
 Paenarthrobacter nicotinovorans succinate semialdehyde + methylamine + H2O2
-
 ?
1.5.3.21 additional information no substrates: spermidine, spermine, sarcosine, dimethylglycine, glycine, choline, betaine, alpha-methylamino isobutyric acid, methylaminopropionnitrile, methylaminopropylamine Paenarthrobacter nicotinovorans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.21 ? x * 46100, calculated, x * 46000, SDS-PAGE Paenarthrobacter nicotinovorans

Synonyms

EC Number Synonyms Comment Organism
1.5.3.21 MAO
-
 Paenarthrobacter nicotinovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.3.21 22
-
 assay at Paenarthrobacter nicotinovorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.21 878
-
 4-aminobutanoate pH 9.8, 22°C Paenarthrobacter nicotinovorans
1.5.3.21 1230
-
 4-methylaminobutanoate pH 9.8, 22°C Paenarthrobacter nicotinovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.21 9.8
-
-
 Paenarthrobacter nicotinovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.21 FAD noncovalently bound Paenarthrobacter nicotinovorans

General Information

EC Number General Information Comment Organism
1.5.3.19 physiological function in nicotine breakdown, two alternative pathways for the final steps in the catabolism of 4-methylaminobutanoate could be identified. 4-Methylaminobutanoate may be demethylated to gamma-N-aminobutyrate by gamma-N-methylaminobutanoate oxidase, EC 1.5.3.19. In an alternative pathway, amine oxidase, EC 1.5.3.21, removes methylamine from 4-methylaminobutanoate with the formation of succinic semialdehyde. Kinetic analyses suggest that demethylation predominates over deamination of 4-methylaminobutanoate Paenarthrobacter nicotinovorans
1.5.3.21 physiological function in nicotine breakdown, two alternative pathways for the final steps in the catabolism of 4-methylaminobutanoate could be identified. 4-Methylaminobutanoate may be demethylated to gamma-N-aminobutyrate by gamma-N-methylaminobutanoate oxidase, EC 1.5.3.19. In an alternative pathway, amine oxidase, EC 1.5.3.21, removes methylamine from 4-methylaminobutanoate with the formation of succinic semialdehyde. Kinetic analyses suggest that demethylation predominates over deamination of 4-methylaminobutanoate Paenarthrobacter nicotinovorans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.3.21 131
-
 4-aminobutanoate pH 9.8, 22°C Paenarthrobacter nicotinovorans
1.5.3.21 490
-
 4-methylaminobutanoate pH 9.8, 22°C Paenarthrobacter nicotinovorans