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Literature summary extracted from

  • Moore, V.; Kanu, A.; Byron, O.; Campbell, G.; Danson, M.J.; Hough, D.W.; Crennell, S.J.
    Contribution of inter-subunit interactions to the thermostability of Pyrococcus furiosus citrate synthase (2011), Extremophiles, 15, 327-336.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.16 expressed in Escherichia coli Pyrococcus furiosus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.3.16 D113A mutant with reduce thermostability Pyrococcus furiosus
2.3.3.16 D113S mutant with reduce thermostability Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.16 43290
-
 2 * 43290, calculated from amino acid sequence Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Pyrococcus furiosus Q53554
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16 Matrex Red Gel A affinity column chromatography Pyrococcus furiosus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.3.16 complete recovery of activity is observed after incubation for 1 h at 50°C by a 20fold dilution in 50 mM phosphate buffer of the enzyme fully unfolded in 6 M guanidine-HCl Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + H2O + oxaloacetate
-
 Pyrococcus furiosus citrate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.16 homodimer 2 * 43290, calculated from amino acid sequence Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
2.3.3.16 citrate synthase
-
 Pyrococcus furiosus