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Literature summary extracted from

  • Pennacchio, A.; Giordano, A.; Pucci, B.; Rossi, M.; Raia, C.A.
    Biochemical characterization of a recombinant short-chain NAD(H)-dependent dehydrogenase/reductase from Sulfolobus acidocaldarius (2010), Extremophiles, 14, 193-204.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.1 1,4-dioxane significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% Sulfolobus acidocaldarius
1.1.1.1 2-mercaptoethanol 5 mM, 112% of initial activity Sulfolobus acidocaldarius
1.1.1.1 2-propanol 120–125% activation after incubation for 25 h in the presence of 17% 2-propanol. High concentration (30%) result in enzyme inactivation to 5–30% of the initial values following 5 h incubation at 50°C Sulfolobus acidocaldarius
1.1.1.1 acetonitrile 120–125% activation after incubation for 25 h in the presence of 17% acetonitrile. High concentration (30%) result in enzyme inactivation to 5–30% of the initial values following 5 h incubation at 50°C Sulfolobus acidocaldarius
1.1.1.1 n-heptane significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% Sulfolobus acidocaldarius
1.1.1.1 n-hexane significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% Sulfolobus acidocaldarius
1.1.1.1 TBME significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% Sulfolobus acidocaldarius

Application

EC Number Application Comment Organism
1.1.1.1 synthesis enzyme catalyses the reduction of alpha-methyl and alpha-ethyl benzoylformate, and methyl o-chlorobenzoylformate with 100% conversion to methyl (S)-mandelate [17% enantiomeric excess (ee)], ethyl (R)-mandelate (50% ee), and methyl (R)-o-chloromandelate (72% ee), respectively, with an efficient in situ NADH-recycling system which involves glucose and a thermophilic glucose dehydrogenase Sulfolobus acidocaldarius

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 expression in Escherichia coli Sulfolobus acidocaldarius
1.1.1.1 overexpressed in Escherichia coli Sulfolobus acidocaldarius

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 1,4-dioxane 30%, 5–30% inactivation of the initial values following 5 h incubation at 50°C Sulfolobus acidocaldarius
1.1.1.1 2-propanol 120–125% activation after incubation for 25 h in the presence of 17% 2-propanol. High concentration (30%) result in enzyme inactivation to 5–30% of the initial values following 5 h incubation at 50°C Sulfolobus acidocaldarius
1.1.1.1 acetonitrile 120–125% activation after incubation for 25 h in the presence of 17% acetonitrile. High concentration (30%) result in enzyme inactivation to 5–30% of the initial values following 5 h incubation at 50°C Sulfolobus acidocaldarius
1.1.1.1 CoSO4 1 mM, 11% inhibition Sulfolobus acidocaldarius
1.1.1.1 Cu2+ 1 mM, 89% of initial activity Sulfolobus acidocaldarius
1.1.1.1 EDTA 1 mM, 93% of initial activity Sulfolobus acidocaldarius
1.1.1.1 FeSO4 1 mM, 20% inhibition Sulfolobus acidocaldarius
1.1.1.1 Hg(CH3COO)2 1 mM, 37% inhibition Sulfolobus acidocaldarius
1.1.1.1 Hg2+ 1 mM, 63% of initial activity Sulfolobus acidocaldarius
1.1.1.1 iodoacetate 1 mM, 12% inhibition Sulfolobus acidocaldarius
1.1.1.1 additional information the chlorides of Li+, Na+, K+, Ca2+, Mg2+, and Mn2+ do not affect the activity of the enzyme, whereas the sulfate of heavy metal ions such as Fe2+, Co2+, and Cu2+ cause a slight inactivation Sulfolobus acidocaldarius
1.1.1.1 NaCl 1 mM, 12% inhibition Sulfolobus acidocaldarius
1.1.1.1 Zn2+ 1 mM, 91% of initial activity Sulfolobus acidocaldarius
1.1.1.1 ZnSO4 1 mM, 9% inhibition Sulfolobus acidocaldarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.16
-
 NADH pH 5.5, 65°C Sulfolobus acidocaldarius
1.1.1.1 0.16
-
 NADH 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 0.44
-
 NAD+ pH 8.0, 65°C Sulfolobus acidocaldarius
1.1.1.1 0.44
-
 NAD+ 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 0.71
-
 isatin pH 5.5, 65°C Sulfolobus acidocaldarius
1.1.1.1 0.71
-
 isatin 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 1.05
-
 (S)-alpha-tetralol pH 8.0, 65°C Sulfolobus acidocaldarius
1.1.1.1 1.05
-
 (S)-alpha-tetralol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 1.28
-
 (R)-alpha-tetralol pH 8.0, 65°C Sulfolobus acidocaldarius
1.1.1.1 1.3
-
 (R)-alpha-tetralol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 2.4
-
 (R)-1-indanol pH 8.0, 65°C Sulfolobus acidocaldarius
1.1.1.1 2.4
-
 (R)-1-indanol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 2.8
-
 methyl o-chlorobenzoylformate pH 5.5, 65°C Sulfolobus acidocaldarius
1.1.1.1 2.8
-
 methyl o-chlorobenzoylformate 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 4.2
-
 cis-decahydro-1-naphthol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 5.3
-
 ethyl benzoylformate 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 6.4
-
 (S)-1-indanol pH 8.0, 65°C Sulfolobus acidocaldarius
1.1.1.1 6.4
-
 (S)-1-indanol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 6.5
-
 methyl benzoylformate 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 9.3
-
 cycloheptanol pH 8.0, 65°C Sulfolobus acidocaldarius
1.1.1.1 9.3
-
 cycloheptanol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 9.9
-
 3-Methylcyclohexanol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 12.1
-
 1-phenyl-1,2-propanedione 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 23.5
-
 2,2,2-trifluoroacetophenone 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 66
-
 3-methylcyclohexanone 65°C, pH 5.5 Sulfolobus acidocaldarius

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 Ni2+ 1 mM, 106% of initial activity Sulfolobus acidocaldarius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 28978
-
 4 * 28978, calculated from sequence Sulfolobus acidocaldarius
1.1.1.1 28978
-
 4 * 28978, calculated, 4 * 30000, SDS-PAGE Sulfolobus acidocaldarius
1.1.1.1 30000
-
 4 * 30000, SDS-PAGE Sulfolobus acidocaldarius
1.1.1.1 30000
-
 4 * 28978, calculated, 4 * 30000, SDS-PAGE Sulfolobus acidocaldarius
1.1.1.1 65000
-
 gel filtration Sulfolobus acidocaldarius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.1 additional information Sulfolobus acidocaldarius the physiological direction of the catalytic reaction is reduction rather than oxidation ?
-
 ?
1.1.1.1 additional information Sulfolobus acidocaldarius DSM 639 the physiological direction of the catalytic reaction is reduction rather than oxidation ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.1 1,4-dioxane 30%, 5–30% inactivation of the initial values following 5 h incubation at 50°C Sulfolobus acidocaldarius
1.1.1.1 1,4-dioxane significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% Sulfolobus acidocaldarius
1.1.1.1 2-propanol 120–125% activation after incubation for 25 h in the presence of 17% 2-propanol. High concentration (30%) result in enzyme inactivation to 5–30% of the initial values following 5 h incubation at 50°C Sulfolobus acidocaldarius
1.1.1.1 acetonitrile 120–125% activation after incubation for 25 h in the presence of 17% acetonitrile. High concentration (30%) result in enzyme inactivation to 5–30% of the initial values following 5 h incubation at 50°C Sulfolobus acidocaldarius
1.1.1.1 dioxane 17%, 160% of initial activity Sulfolobus acidocaldarius
1.1.1.1 Ethyl acetate significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% Sulfolobus acidocaldarius
1.1.1.1 Ethyl acetate 30%, 180% of initial activity Sulfolobus acidocaldarius
1.1.1.1 methyl-tert-butylether significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% Sulfolobus acidocaldarius
1.1.1.1 n-heptane significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% Sulfolobus acidocaldarius
1.1.1.1 n-heptane 30%, 180% of initial activity Sulfolobus acidocaldarius
1.1.1.1 n-hexane significant increases in enzyme activity occurs after 25 h incubation at a concentration of 30% Sulfolobus acidocaldarius
1.1.1.1 tert-butylmethyl ether 30%, 210% of initial activity Sulfolobus acidocaldarius

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Sulfolobus acidocaldarius Q4J702
-
-
1.1.1.1 Sulfolobus acidocaldarius Q4J9F2
-
-
1.1.1.1 Sulfolobus acidocaldarius DSM 639 Q4J702
-
-
1.1.1.1 Sulfolobus acidocaldarius DSM 639 Q4J9F2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1
-
 Sulfolobus acidocaldarius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 6.6
-
 pH 5.5, 65°C Sulfolobus acidocaldarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 (R)-1-indanol + NAD+ 62% of activity compared to (S)-1-indanol Sulfolobus acidocaldarius ?
-
 ?
1.1.1.1 (R)-1-indanol + NAD+
-
 Sulfolobus acidocaldarius 1-indanone + NADH + H+
-
 r
1.1.1.1 (R)-alpha-tetralol + NAD+ 58% of activity compared to (S)-1-indanol Sulfolobus acidocaldarius ?
-
 ?
1.1.1.1 (R)-alpha-tetralol + NAD+
-
 Sulfolobus acidocaldarius alpha-tetralone + NADH + H+
-
 r
1.1.1.1 (S)-1-indanol + NAD+
-
 Sulfolobus acidocaldarius ?
-
 ?
1.1.1.1 (S)-1-indanol + NAD+
-
 Sulfolobus acidocaldarius 1-indanone + NADH + H+
-
 r
1.1.1.1 (S)-2-pentanol + NAD+ 11% of activity compared to (S)-1-indanol Sulfolobus acidocaldarius ?
-
 ?
1.1.1.1 (S)-alpha-tetralol + NAD+
-
 Sulfolobus acidocaldarius alpha-tetralone + NADH + H+
-
 r
1.1.1.1 (S)-alpha-tetralol + NAD+
-
 Sulfolobus acidocaldarius DSM 639 alpha-tetralone + NADH + H+
-
 r
1.1.1.1 (S)-alpha-tetralol + NAD+ 12% of activity compared to (S)-1-indanol Sulfolobus acidocaldarius ?
-
 ?
1.1.1.1 1-indanone + NADH + H+
-
 Sulfolobus acidocaldarius (S)-1-indanol + NAD+ 6 h, 50°C, 6% conversion, 25% enantiomeric excess r
1.1.1.1 1-indanone + NADH + H+
-
 Sulfolobus acidocaldarius DSM 639 (S)-1-indanol + NAD+ 6 h, 50°C, 6% conversion, 25% enantiomeric excess r
1.1.1.1 1-phenyl-1,2-propanedione + NADH about 25% of the activity compared to isatin Sulfolobus acidocaldarius ?
-
 ?
1.1.1.1 2,2,2-trifluoroacetophenone + NADH about 35% of the activity compared to isatin Sulfolobus acidocaldarius 2,2,2-trifluoro-1-phenylethanol + NAD+
-
 ?
1.1.1.1 3-methylcyclohexanol + NAD+ 14% of activity compared to (S)-1-indanol Sulfolobus acidocaldarius 3-methylcyclohexanone + NADH + H+
-
 ?
1.1.1.1 3-methylcyclohexanone + NADH about 10% of the activity compared to isatin Sulfolobus acidocaldarius 3-methylcyclohexanol + NAD+
-
 ?
1.1.1.1 3-methylcyclohexanone + NADH about 10% of the activity compared to isatin Sulfolobus acidocaldarius DSM 639 3-methylcyclohexanol + NAD+
-
 ?
1.1.1.1 cis-decahydro-1-naphthol + NAD+ 11% of activity compared to (S)-1-indanol Sulfolobus acidocaldarius ?
-
 ?
1.1.1.1 cycloheptanol + NAD+
-
 Sulfolobus acidocaldarius cycloheptanone + NADH + H+
-
 r
1.1.1.1 cycloheptanol + NAD+ 37% of activity compared to (S)-1-indanol Sulfolobus acidocaldarius cycloheptanone + NADH + H+
-
 ?
1.1.1.1 ethyl benzoylformate + NADH about 30% of the activity compared to isatin Sulfolobus acidocaldarius ?
-
 ?
1.1.1.1 ethyl benzoylformate + NADH + H+
-
 Sulfolobus acidocaldarius ethyl (R)-mandelate + NAD+ 50% enantiomeric excess r
1.1.1.1 ethyl benzoylformate + NADH + H+
-
 Sulfolobus acidocaldarius DSM 639 ethyl (R)-mandelate + NAD+ 50% enantiomeric excess r
1.1.1.1 ethyl pyruvate + NADH about 15% of the activity compared to isatin Sulfolobus acidocaldarius ?
-
 ?
1.1.1.1 ethyl pyruvate + NADH about 15% of the activity compared to isatin Sulfolobus acidocaldarius DSM 639 ?
-
 ?
1.1.1.1 isatin + NADH
-
 Sulfolobus acidocaldarius ?
-
 ?
1.1.1.1 isatin + NADH + H+
-
 Sulfolobus acidocaldarius ? + NAD+
-
 r
1.1.1.1 isatin + NADH + H+
-
 Sulfolobus acidocaldarius DSM 639 ? + NAD+
-
 r
1.1.1.1 methyl benzoylformate + NADH about 35% of the activity compared to isatin Sulfolobus acidocaldarius ?
-
 ?
1.1.1.1 methyl benzoylformate + NADH about 35% of the activity compared to isatin Sulfolobus acidocaldarius DSM 639 ?
-
 ?
1.1.1.1 methyl benzoylformate + NADH + H+
-
 Sulfolobus acidocaldarius methyl (S)-mandelate + NAD+ 6 h, 100% conversion, 17% enantiomeric excess r
1.1.1.1 methyl o-chlorobenzoylformate + NADH about 55% of the activity compared to isatin Sulfolobus acidocaldarius ?
-
 ?
1.1.1.1 methyl o-chlorobenzoylformate + NADH + H+
-
 Sulfolobus acidocaldarius methyl (R)-o-chloromandelate + NAD+ 6 h, 99% conversion, 72% enantiomeric excess r
1.1.1.1 additional information the physiological direction of the catalytic reaction is reduction rather than oxidation Sulfolobus acidocaldarius ?
-
 ?
1.1.1.1 additional information the enzyme shows no activity on aliphatic linear and branched alcohols, except for a poor activity on 2-propyn-1-ol, 3-methyl-1-butanol and 2-pentanol; however, it shows a discrete activity on aliphatic cyclic and bicyclic alcohols. Benzyl alcohol and 4-bromobenzyl alcohol are not found to be substrates. The S and R enantiomers of a-(trifluoromethyl)benzyl alcohol and methyl and ethyl mandelates show no apparent activity with SaADH. The enzyme shows poor activity on (+/-)-1-phenyl-1-propanol, 1-(1-naphthyl)ethanol and the two enantiomers of 1-(2-naphthyl)ethanol. The enzyme is not active on aliphatic and aromatic aldehydes, and on aliphatic linear, branched and cyclic ketones except for 3-methylcyclohexanone. Catalytic inactivity is observed with acetophenone and (S)-a-(trifluoromethyl)benzyl Sulfolobus acidocaldarius ?
-
 ?
1.1.1.1 additional information enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-keto esters, but is poorly active on aliphatic, cyclic and aromatic alcohols, and shows no activity on aldehydes Sulfolobus acidocaldarius ?
-
 ?
1.1.1.1 additional information the physiological direction of the catalytic reaction is reduction rather than oxidation Sulfolobus acidocaldarius DSM 639 ?
-
 ?
1.1.1.1 additional information the enzyme shows no activity on aliphatic linear and branched alcohols, except for a poor activity on 2-propyn-1-ol, 3-methyl-1-butanol and 2-pentanol; however, it shows a discrete activity on aliphatic cyclic and bicyclic alcohols. Benzyl alcohol and 4-bromobenzyl alcohol are not found to be substrates. The S and R enantiomers of a-(trifluoromethyl)benzyl alcohol and methyl and ethyl mandelates show no apparent activity with SaADH. The enzyme shows poor activity on (+/-)-1-phenyl-1-propanol, 1-(1-naphthyl)ethanol and the two enantiomers of 1-(2-naphthyl)ethanol. The enzyme is not active on aliphatic and aromatic aldehydes, and on aliphatic linear, branched and cyclic ketones except for 3-methylcyclohexanone. Catalytic inactivity is observed with acetophenone and (S)-a-(trifluoromethyl)benzyl Sulfolobus acidocaldarius DSM 639 ?
-
 ?
1.1.1.1 additional information enzyme displays a preference for the reduction of alicyclic, bicyclic and aromatic ketones and alpha-keto esters, but is poorly active on aliphatic, cyclic and aromatic alcohols, and shows no activity on aldehydes Sulfolobus acidocaldarius DSM 639 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 More tetramer structure results from chemical crosslinking experiments Sulfolobus acidocaldarius
1.1.1.1 tetramer 4 * 30000, SDS-PAGE Sulfolobus acidocaldarius
1.1.1.1 tetramer 4 * 28978, calculated from sequence Sulfolobus acidocaldarius
1.1.1.1 tetramer 4 * 28978, calculated, 4 * 30000, SDS-PAGE Sulfolobus acidocaldarius

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 SaADH
-
 Sulfolobus acidocaldarius
1.1.1.1 Saci_1232
-
 Sulfolobus acidocaldarius
1.1.1.1 short-chain ADH
-
 Sulfolobus acidocaldarius
1.1.1.1 short-chain NAD(H)-dependent dehydrogenase/reductase
-
 Sulfolobus acidocaldarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 65
-
 assay at Sulfolobus acidocaldarius
1.1.1.1 75
-
-
 Sulfolobus acidocaldarius

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.1 65 85 65°C: about 40% of maximal activity, 85°C: about 35% of maximal activity Sulfolobus acidocaldarius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 50
-
 24 h, 109% of initial activity Sulfolobus acidocaldarius
1.1.1.1 70
-
 pH 9.0, 50 mM Tris-HCl, 24% loss of acrtivity Sulfolobus acidocaldarius
1.1.1.1 70
-
 24 h, 76% of initial activity Sulfolobus acidocaldarius
1.1.1.1 90
-
 half-life 30 min Sulfolobus acidocaldarius
1.1.1.1 90
-
 half-inactivation temperature is 30 min Sulfolobus acidocaldarius

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.1 0.98
-
 cis-decahydro-1-naphthol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 2 8 NADH pH 5.5, 65°C Sulfolobus acidocaldarius
1.1.1.1 2 8 NADH 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 2.1
-
 (S)-alpha-tetralol pH 8.0, 65°C Sulfolobus acidocaldarius
1.1.1.1 2.1
-
 (S)-alpha-tetralol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 2.35
-
 ethyl benzoylformate 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 3.1
-
 1-phenyl-1,2-propanedione 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 3.7
-
 NAD+ pH 8.0, 65°C Sulfolobus acidocaldarius
1.1.1.1 3.7
-
 NAD+ 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 3.8
-
 3-methylcyclohexanone 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 4.4
-
 cycloheptanol pH 8.0, 65°C Sulfolobus acidocaldarius
1.1.1.1 4.4
-
 cycloheptanol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 6.2
-
 methyl benzoylformate 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 7.1
-
 (R)-1-indanol pH 8.0, 65°C Sulfolobus acidocaldarius
1.1.1.1 7.1
-
 (R)-1-indanol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 9.2
-
 methyl o-chlorobenzoylformate pH 5.5, 65°C Sulfolobus acidocaldarius
1.1.1.1 9.2
-
 methyl o-chlorobenzoylformate 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 10
-
 2,2,2-trifluoroacetophenone 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 13.5
-
 (R)-alpha-tetralol pH 8.0, 65°C Sulfolobus acidocaldarius
1.1.1.1 13.5
-
 (R)-alpha-tetralol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 13.7
-
 (S)-1-indanol pH 8.0, 65°C Sulfolobus acidocaldarius
1.1.1.1 13.7
-
 (S)-1-indanol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 22
-
 isatin pH 5.5, 65°C Sulfolobus acidocaldarius
1.1.1.1 22
-
 isatin 65°C, pH 5.5 Sulfolobus acidocaldarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 5.1
-
 reduction reaction Sulfolobus acidocaldarius
1.1.1.1 5.1
-
 reduction of ketones Sulfolobus acidocaldarius
1.1.1.1 5.5
-
 assay at Sulfolobus acidocaldarius
1.1.1.1 8.2
-
 oxidation reaction Sulfolobus acidocaldarius
1.1.1.1 8.2
-
 oxidation of alcohols Sulfolobus acidocaldarius

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.1 4.9 5.8 pH 4.9: about 25% of maximal activity, pH 5.8: about 40% of maximal activity, reduction reaction Sulfolobus acidocaldarius
1.1.1.1 6.7 8.5 pH 6.7: about 40% of maximal activity, pH 8.5: about 85% of maximal activity, oxidation reaction Sulfolobus acidocaldarius

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+ the enzyme transfers the deuteride to the Si-face of NAD+ Sulfolobus acidocaldarius
1.1.1.1 NADH strictly required Sulfolobus acidocaldarius
1.1.1.1 NADH the specificity constant value is 21-fold higher for NADH than NAD+. No activity with NADP(H) Sulfolobus acidocaldarius

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.1 0.06
-
 3-methylcyclohexanone 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 0.23
-
 cis-decahydro-1-naphthol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 0.25
-
 1-phenyl-1,2-propanedione 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 0.42
-
 2,2,2-trifluoroacetophenone 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 0.44
-
 ethyl benzoylformate 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 0.47
-
 cycloheptanol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 0.47
-
 cycloheptanol pH 8.0, 65°C Sulfolobus acidocaldarius
1.1.1.1 0.95
-
 methyl benzoylformate 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 2
-
 (S)-alpha-tetralol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 2
-
 (S)-alpha-tetralol pH 8.0, 65°C Sulfolobus acidocaldarius
1.1.1.1 2.14
-
 (S)-1-indanol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 2.14
-
 (S)-1-indanol pH 8.0, 65°C Sulfolobus acidocaldarius
1.1.1.1 2.98
-
 (R)-1-indanol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 2.98
-
 (R)-1-indanol pH 8.0, 65°C Sulfolobus acidocaldarius
1.1.1.1 3.3
-
 methyl o-chlorobenzoylformate pH 5.5, 65°C Sulfolobus acidocaldarius
1.1.1.1 3.3
-
 methyl o-chlorobenzoylformate 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 8.4
-
 NAD+ 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 8.4
-
 NAD+ pH 8.0, 65°C Sulfolobus acidocaldarius
1.1.1.1 10.5
-
 (R)-alpha-tetralol 65°C, pH 8.0 Sulfolobus acidocaldarius
1.1.1.1 10.5
-
 (R)-alpha-tetralol pH 8.0, 65°C Sulfolobus acidocaldarius
1.1.1.1 31
-
 isatin pH 5.5, 65°C Sulfolobus acidocaldarius
1.1.1.1 31
-
 isatin 65°C, pH 5.5 Sulfolobus acidocaldarius
1.1.1.1 175
-
 NADH pH 5.5, 65°C Sulfolobus acidocaldarius
1.1.1.1 175
-
 NADH 65°C, pH 5.5 Sulfolobus acidocaldarius