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Literature summary extracted from

  • Ruiz, A.; Bok, D.
    Focus on molecules: lecithin retinol acyltransferase (2010), Exp. Eye Res., 90, 186-187.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.135 gene lrat is localized on chromosome 4 at locus 4q31.2 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.135 additional information
-
additional information steady-state kinetic analysis using purified truncated mutant tLRAT Homo sapiens
2.3.1.135 0.00024
-
all-trans-retinol-[cellular-retinol-binding-protein] pH and temperature not specified in the publication Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.135 membrane boound Mus musculus 16020
-
2.3.1.135 membrane boound Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.135 25000
-
x * 25000, about Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.135 phosphatidylcholine + 11-cis-retinol-[cellular-retinol-binding-protein] Homo sapiens trans-esterification reaction is reversible, however in the presence of the isomerase, all-trans-retinyl esters are converted to 11-cis-retinol which is enzymatically oxidized to 11-cis-retinal, the chromophore of vision. Both all-trans-retinol and 11-cis-retinol are substrates for LRAT esterification, although all-transretinol is the preferred substrate. 2-acylglycerophosphocholine + 11-cis-retinyl-ester-[cellular-retinol-binding-protein]
-
r
2.3.1.135 phosphatidylcholine + 11-cis-retinoll-[cellular-retinol-binding-protein] Mus musculus trans-esterification reaction is reversible, however in the presence of the isomerase, all-trans-retinyl esters are converted to 11-cis-retinol which is enzymatically oxidized to 11-cis-retinal, the chromophore of vision. Both all-trans-retinol and 11-cis-retinol are substrates for LRAT esterification, although all-transretinol is the preferred substrate. 2-acylglycerophosphocholine + 11-cis-retinyl-ester-[cellular-retinol-binding-protein]
-
r
2.3.1.135 phosphatidylcholine + all-trans-retinol-[cellular-retinol-binding-protein] Mus musculus trans-esterification reaction is reversible, however in the presence of the isomerase, all-trans-retinyl esters are converted to 11-cis-retinol which is enzymatically oxidized to 11-cis-retinal, the chromophore of vision. Both all-trans-retinol and 11-cis-retinol are substrates for LRAT esterification, although all-transretinol is the preferred substrate. 2-acylglycerophosphocholine + all-trans-retinyl-ester-[cellular-retinol-binding-protein]
-
r
2.3.1.135 phosphatidylcholine + all-trans-retinol-[cellular-retinol-binding-protein] Homo sapiens trans-esterification reaction is reversible, however in the presence of the isomerase, all-trans-retinyl esters are converted to 11-cis-retinol which is enzymatically oxidized to 11-cis-retinal, the chromophore of vision. Both all-trans-retinol and 11-cis-retinol are substrates for LRAT esterification, although all-transretinol is the preferred substrate. 2-acylglycerophosphocholine + all-trans-retinyl-ester-[cellular-retinol-binding-protein]
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.135 Homo sapiens
-
gene Lrat
-
2.3.1.135 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.135 eye
-
Mus musculus
-
2.3.1.135 eye
-
Homo sapiens
-
2.3.1.135 additional information LRAT is expressed in multiple tissues Mus musculus
-
2.3.1.135 additional information LRAT is expressed in multiple tissues Homo sapiens
-
2.3.1.135 retinal pigment epithelium
-
Mus musculus
-
2.3.1.135 retinal pigment epithelium
-
Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.135 phosphatidylcholine + 11-cis-retinol-[cellular-retinol-binding-protein] trans-esterification reaction is reversible, however in the presence of the isomerase, all-trans-retinyl esters are converted to 11-cis-retinol which is enzymatically oxidized to 11-cis-retinal, the chromophore of vision. Both all-trans-retinol and 11-cis-retinol are substrates for LRAT esterification, although all-transretinol is the preferred substrate. Homo sapiens 2-acylglycerophosphocholine + 11-cis-retinyl-ester-[cellular-retinol-binding-protein]
-
r
2.3.1.135 phosphatidylcholine + 11-cis-retinol-[cellular-retinol-binding-protein] both all-trans-retinol and 11-cis-retinol are substrates for LRAT esterification, although all-transretinol is the preferred substrate. Mus musculus 2-acylglycerophosphocholine + 11-cis-retinyl-ester-[cellular-retinol-binding-protein]
-
r
2.3.1.135 phosphatidylcholine + 11-cis-retinol-[cellular-retinol-binding-protein] both all-trans-retinol and 11-cis-retinol are substrates for LRAT esterification, although all-transretinol is the preferred substrate. Homo sapiens 2-acylglycerophosphocholine + 11-cis-retinyl-ester-[cellular-retinol-binding-protein]
-
r
2.3.1.135 phosphatidylcholine + 11-cis-retinoll-[cellular-retinol-binding-protein] trans-esterification reaction is reversible, however in the presence of the isomerase, all-trans-retinyl esters are converted to 11-cis-retinol which is enzymatically oxidized to 11-cis-retinal, the chromophore of vision. Both all-trans-retinol and 11-cis-retinol are substrates for LRAT esterification, although all-transretinol is the preferred substrate. Mus musculus 2-acylglycerophosphocholine + 11-cis-retinyl-ester-[cellular-retinol-binding-protein]
-
r
2.3.1.135 phosphatidylcholine + all-trans-retinol-[cellular-retinol-binding-protein] trans-esterification reaction is reversible, however in the presence of the isomerase, all-trans-retinyl esters are converted to 11-cis-retinol which is enzymatically oxidized to 11-cis-retinal, the chromophore of vision. Both all-trans-retinol and 11-cis-retinol are substrates for LRAT esterification, although all-transretinol is the preferred substrate. Mus musculus 2-acylglycerophosphocholine + all-trans-retinyl-ester-[cellular-retinol-binding-protein]
-
r
2.3.1.135 phosphatidylcholine + all-trans-retinol-[cellular-retinol-binding-protein] trans-esterification reaction is reversible, however in the presence of the isomerase, all-trans-retinyl esters are converted to 11-cis-retinol which is enzymatically oxidized to 11-cis-retinal, the chromophore of vision. Both all-trans-retinol and 11-cis-retinol are substrates for LRAT esterification, although all-transretinol is the preferred substrate. Homo sapiens 2-acylglycerophosphocholine + all-trans-retinyl-ester-[cellular-retinol-binding-protein]
-
r
2.3.1.135 phosphatidylcholine + all-trans-retinol-[cellular-retinol-binding-protein] both all-trans-retinol and 11-cis-retinol are substrates for LRAT esterification, although all-transretinol is the preferred substrate. Mus musculus 2-acylglycerophosphocholine + all-trans-retinyl-ester-[cellular-retinol-binding-protein]
-
r
2.3.1.135 phosphatidylcholine + all-trans-retinol-[cellular-retinol-binding-protein] both all-trans-retinol and 11-cis-retinol are substrates for LRAT esterification, although all-transretinol is the preferred substrate. Homo sapiens 2-acylglycerophosphocholine + all-trans-retinyl-ester-[cellular-retinol-binding-protein]
-
r

Subunits

EC Number Subunits Comment Organism
2.3.1.135 ? x * 25000, about Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.3.1.135 lecithin retinol acyltransferase
-
Mus musculus
2.3.1.135 lecithin retinol acyltransferase
-
Homo sapiens
2.3.1.135 LRAT
-
Mus musculus
2.3.1.135 LRAT
-
Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.1.135 evolution based on its secondary structure LRAT belongs to a superfamily of enzymes generically referred as NIpC/P60. Within this superfamily, a multiple sequence alignment of LRAT and LRAT-like family members shows that they share three conserved amino acid residues; cysteine, histidine and a polar residue that is thought to complete a catalytic triad similar to the papain-like thiol peptidases Mus musculus
2.3.1.135 evolution based on its secondary structure LRAT belongs to a superfamily of enzymes generically referred as NIpC/P60. Within this superfamily, a multiple sequence alignment of LRAT and LRAT-like family members shows that they share three conserved amino acid residues; cysteine, histidine and a polar residue that is thought to complete a catalytic triad similar to the papain-like thiol peptidases Homo sapiens
2.3.1.135 malfunction generation of an animal model in which the lrat gene is disrupted by homologous recombination gives Lrat-/- mice, which show slow degeneration of their retinas, essentially a shortening of rod outer segments and highly attenuated electroretinograms Mus musculus
2.3.1.135 malfunction homozygous mutation S175R occurs in two patients diagnosed with severe early-onset retinal degeneration Homo sapiens
2.3.1.135 physiological function function of LRAT is to catalyze a trans-esterification reaction that occurs between the sn-1 position of lecithin molecules in the lipid bilayer of the smooth endoplasmic reticulum and all-trans-retinol in the formation of all-trans-retinyl esters. Functional role of LRAT in the visual cycle Mus musculus
2.3.1.135 physiological function function of LRAT is to catalyze a trans-esterification reaction that occurs between the sn-1 position of lecithin molecules in the lipid bilayer of the smooth endoplasmic reticulum and all-trans-retinol in the formation of all-trans-retinyl esters. Functional role of LRAT in the visual cycle Homo sapiens