Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chiribau, C.; Sandu, C.; Fraaije, M.; Schiltz, E.; Brandsch, R.
    A novel gamma-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1 (2004), Eur. J. Biochem., 271, 4677-4684.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.19 expression in Escherichia coli Paenarthrobacter nicotinovorans

Protein Variants

EC Number Protein Variants Comment Organism
1.5.3.19 H67A mutation results in resulted in a protein without covalently bound flavin. Mutant protein contains noncovalently bound flavin and exhibits 10% of the enzyme activity of the wild-type Paenarthrobacter nicotinovorans
1.5.3.19 W66S mutation results in resulted in a protein without covalently bound flavin and complete loss of actiovity Paenarthrobacter nicotinovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.19 0.14
-
 4-methylaminobutanoate pH 10, temperature not specified in the publication Paenarthrobacter nicotinovorans
1.5.3.19 25
-
 sarcosine pH 10, temperature not specified in the publication Paenarthrobacter nicotinovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.19 89395
-
 1 * 90000, SDS-PAGE, 1 * 89395, calculated Paenarthrobacter nicotinovorans
1.5.3.19 90000
-
 gel filtration Paenarthrobacter nicotinovorans

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.19 Paenarthrobacter nicotinovorans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.19 4-methylaminobutanoate + O2 + H2O
-
 Paenarthrobacter nicotinovorans 4-aminobutanoate + formaldehyde + H2O2
-
 ?
1.5.3.19 additional information no substrate: dimethylglycine, 2-methylaminoethanol, methylaminopropylamine, methylaminopropionnitrile, 12-(methylamino)lauric acid Paenarthrobacter nicotinovorans ?
-
 ?
1.5.3.19 sarcosine + O2 + H2O about 200fold decrease in rate compared to 4-methylaminobutanoate Paenarthrobacter nicotinovorans glycine + formaldehyde + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.19 monomer 1 * 90000, SDS-PAGE, 1 * 89395, calculated Paenarthrobacter nicotinovorans

Synonyms

EC Number Synonyms Comment Organism
1.5.3.19 gamma-N-methylaminobutanoate oxidase
-
 Paenarthrobacter nicotinovorans
1.5.3.19 gamma-N-methylaminobutyrate oxidase
-
 Paenarthrobacter nicotinovorans
1.5.3.19 mabO
-
 Paenarthrobacter nicotinovorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.19 4
-
 sarcosine pH 10, temperature not specified in the publication Paenarthrobacter nicotinovorans
1.5.3.19 800
-
 4-methylaminobutanoate pH 10, temperature not specified in the publication Paenarthrobacter nicotinovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.19 8 10
-
 Paenarthrobacter nicotinovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.19 flavin 1 flavin molecule per protein molecule, flavin is covalently attached. Enzyme is able to perform oxidation reactions which involve a 2-electron reduction of the flavin cofactor Paenarthrobacter nicotinovorans

Expression

EC Number Organism Comment Expression
1.5.3.19 Paenarthrobacter nicotinovorans expression only upon growth in presence of nicotine up