Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Cacciapuoti, G.; Porcelli, M.; Carteni-Farina, M.; Gambacorta, A.; Zappia, V.
    Purification and characterization of propylamine transferase from Sulfolobus solfataricus, an extreme thermophilic archaebacterium (1986), Eur. J. Biochem., 161, 263-271.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.23 5'-isobutylthioadenosine
-
 Saccharolobus solfataricus
2.5.1.23 5'-methylthiotubercidin
-
 Saccharolobus solfataricus
2.5.1.23 5'-S-ethyl-5'-thioadenosine
-
 Saccharolobus solfataricus
2.5.1.23 5'-S-methyl-5'-thioadenosine powerful competitive inhibitor Saccharolobus solfataricus
2.5.1.23 A9154C
-
 Saccharolobus solfataricus
2.5.1.23 n-propylthioadenosine
-
 Saccharolobus solfataricus
2.5.1.23 S-adenosyl(5')-3-thiopropylamine
-
 Saccharolobus solfataricus
2.5.1.23 S-adenosyl-3-thio-1,8-diaminooctane
-
 Saccharolobus solfataricus
2.5.1.23 S-adenosyl-3-thiopropylamine
-
 Saccharolobus solfataricus
2.5.1.23 sinefungin
-
 Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.23 0.0079
-
 S-adenosylmethioninamine pH 7.5, 78°C Saccharolobus solfataricus
2.5.1.23 0.954
-
 sym-norspermidine pH 7.5, 78°C Saccharolobus solfataricus
2.5.1.23 1.539
-
 spermidine pH 7.5, 78°C Saccharolobus solfataricus
2.5.1.23 1.675
-
 propane-1,3-diamine pH 7.5, 78°C Saccharolobus solfataricus
2.5.1.23 3.85
-
 putrescine pH 7.5, 78°C Saccharolobus solfataricus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.23 cytosol no activity is associated with the cellular particulate fraction Saccharolobus solfataricus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.23 35000
-
 3 * 35000, SDS-PAGE Saccharolobus solfataricus
2.5.1.23 110000
-
 gel filtration, ultracentrifugation analysis Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.23 Saccharolobus solfataricus
-
-
-
2.5.1.23 Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.23
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.23 2.059
-
 pH 7.5, 78°C Saccharolobus solfataricus

Storage Stability

EC Number Storage Stability Organism
2.5.1.23 -20°C, pH 7.5, stable for 1 months Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.23 S-adenosylmethioninamine + propane-1,3-diamine
-
 Saccharolobus solfataricus 5'-S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
-
 ir
2.5.1.23 S-adenosylmethioninamine + propane-1,3-diamine
-
 Saccharolobus solfataricus MT-4 / DSM 5833 5'-S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
-
 ir
2.5.1.23 S-adenosylmethioninamine + putrescine 36.7% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism Saccharolobus solfataricus 5'-S-methyl-5'-thioadenosine + spermidine
-
 ir
2.5.1.23 S-adenosylmethioninamine + putrescine 36.7% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism Saccharolobus solfataricus MT-4 / DSM 5833 5'-S-methyl-5'-thioadenosine + spermidine
-
 ir
2.5.1.23 S-adenosylmethioninamine + spermidine 6.8% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism Saccharolobus solfataricus 5'-S-methyl-5'-thioadenosine + spermine
-
 ir
2.5.1.23 S-adenosylmethioninamine + spermidine 6.8% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism Saccharolobus solfataricus MT-4 / DSM 5833 5'-S-methyl-5'-thioadenosine + spermine
-
 ir
2.5.1.23 S-adenosylmethioninamine + sym-norspermidine 17.5% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism Saccharolobus solfataricus 5'-S-methyl-5'-thioadenosine + ?
-
 ir
2.5.1.23 S-adenosylmethioninamine + sym-norspermidine 17.5% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism Saccharolobus solfataricus MT-4 / DSM 5833 5'-S-methyl-5'-thioadenosine + ?
-
 ir

Subunits

EC Number Subunits Comment Organism
2.5.1.23 trimer 3 * 35000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.23 78
-
 assay at Saccharolobus solfataricus
2.5.1.23 90
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.23 50 110 50°C: about 40% of maximal activity, 110°C: about 45% of maximal activity, 120°C: about 20% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.23 100
-
 1 h, completely stable Saccharolobus solfataricus
2.5.1.23 120
-
 no protection by substrate against thermal inactivation at 120°C is observed by incubating the enzyme in the presence of polyamines Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.23 7.5
-
-
 Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.23 6 9.5 pH 6: about 50% of maximal activity, pH 9.5: about 50% of maximal activity Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.23 0.0037
-
 5'-S-methyl-5'-thioadenosine pH 7.5, 78°C Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.5.1.23 Saccharolobus solfataricus isoelectric focusing
-
 5.3

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.23 0.014
-
 pH 7.5, 78°C Saccharolobus solfataricus 5'-S-methyl-5'-thioadenosine
2.5.1.23 0.016
-
 pH 7.5, 78°C Saccharolobus solfataricus S-adenosyl-3-thiopropylamine
2.5.1.23 0.021
-
 pH 7.5, 78°C Saccharolobus solfataricus S-adenosyl-3-thio-1,8-diaminooctane
2.5.1.23 0.1
-
 pH 7.5, 78°C Saccharolobus solfataricus sinefungin
2.5.1.23 0.1
-
 pH 7.5, 78°C Saccharolobus solfataricus 5'-S-ethyl-5'-thioadenosine
2.5.1.23 0.1
-
 pH 7.5, 78°C Saccharolobus solfataricus A9154C
2.5.1.23 0.12
-
 pH 7.5, 78°C Saccharolobus solfataricus n-propylthioadenosine
2.5.1.23 0.15
-
 pH 7.5, 78°C Saccharolobus solfataricus 5'-isobutylthioadenosine

Expression

EC Number Organism Comment Expression
2.5.1.23 Saccharolobus solfataricus no difference in its activity between the cells harvested during the logarithmic or the stationary phase of growth. Propylamine transferase is not induced by the addition of either 1 mM 1,3-diaminopropane or sym-norspermidine or spermidine to the standard culture medium additional information