BRENDA - Enzyme Database

Biosynthesis of (+)-catechin glycosides using recombinant amylosucrase from Deinococcus geothermalis DSM 11300

Cho, H.K.; Kim, H.H.; Seo, D.H.; Jung, J.H.; Park, J.H.; Baek, N.I.; Kim, M.J.; Yoo, S.H.; Cha, J.; Kim, Y.R.; Park, C.S.; Enzyme Microb. Technol. 49, 246-253 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.4.1.4
gene dgas, expression of GST-tagged DGAS in Escherichia coli
Deinococcus geothermalis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.4
sucrose + [(1->4)-alpha-D-glucosyl]n
Deinococcus geothermalis
-
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
?
2.4.1.4
sucrose + [(1->4)-alpha-D-glucosyl]n
Deinococcus geothermalis DSM 11300
-
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.4
Deinococcus geothermalis
Q1J0W0
gene dgas
-
2.4.1.4
Deinococcus geothermalis DSM 11300
Q1J0W0
gene dgas
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.4
recombinant GST-tagged DGAS from Escherichia coli by glutathione affinity chromatography
Deinococcus geothermalis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.4
additional information
NMR product analysis, overview
719376
Deinococcus geothermalis
?
-
-
-
-
2.4.1.4
additional information
NMR product analysis, overview
719376
Deinococcus geothermalis DSM 11300
?
-
-
-
-
2.4.1.4
sucrose + (+)-catechin
-
719376
Deinococcus geothermalis
D-fructose + (+)-catechin-3'-O-alpha-D-glucopyranoside
-
-
-
?
2.4.1.4
sucrose + (+)-catechin
-
719376
Deinococcus geothermalis DSM 11300
D-fructose + (+)-catechin-3'-O-alpha-D-glucopyranoside
-
-
-
?
2.4.1.4
sucrose + maltose
the enzyme also produces (+)-catechin maltooligosaccharides
719376
Deinococcus geothermalis
D-fructose + (+)-catechin-3'-O-alpha-D-maltoside
-
-
-
?
2.4.1.4
sucrose + maltose
the enzyme also produces (+)-catechin maltooligosaccharides
719376
Deinococcus geothermalis DSM 11300
D-fructose + (+)-catechin-3'-O-alpha-D-maltoside
-
-
-
?
2.4.1.4
sucrose + [(1->4)-alpha-D-glucosyl]n
-
719376
Deinococcus geothermalis
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
2.4.1.4
sucrose + [(1->4)-alpha-D-glucosyl]n
a phenolic aglycone compound can also act an acceptor molecule of ASase transglycosylation activity
719376
Deinococcus geothermalis
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
2.4.1.4
sucrose + [(1->4)-alpha-D-glucosyl]n
-
719376
Deinococcus geothermalis DSM 11300
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
2.4.1.4
sucrose + [(1->4)-alpha-D-glucosyl]n
a phenolic aglycone compound can also act an acceptor molecule of ASase transglycosylation activity
719376
Deinococcus geothermalis DSM 11300
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.4
45
-
assay at
Deinococcus geothermalis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.4
7
-
assay at
Deinococcus geothermalis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.4
gene dgas, expression of GST-tagged DGAS in Escherichia coli
Deinococcus geothermalis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.4
sucrose + [(1->4)-alpha-D-glucosyl]n
Deinococcus geothermalis
-
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
?
2.4.1.4
sucrose + [(1->4)-alpha-D-glucosyl]n
Deinococcus geothermalis DSM 11300
-
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.4
recombinant GST-tagged DGAS from Escherichia coli by glutathione affinity chromatography
Deinococcus geothermalis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.4
additional information
NMR product analysis, overview
719376
Deinococcus geothermalis
?
-
-
-
-
2.4.1.4
additional information
NMR product analysis, overview
719376
Deinococcus geothermalis DSM 11300
?
-
-
-
-
2.4.1.4
sucrose + (+)-catechin
-
719376
Deinococcus geothermalis
D-fructose + (+)-catechin-3'-O-alpha-D-glucopyranoside
-
-
-
?
2.4.1.4
sucrose + (+)-catechin
-
719376
Deinococcus geothermalis DSM 11300
D-fructose + (+)-catechin-3'-O-alpha-D-glucopyranoside
-
-
-
?
2.4.1.4
sucrose + maltose
the enzyme also produces (+)-catechin maltooligosaccharides
719376
Deinococcus geothermalis
D-fructose + (+)-catechin-3'-O-alpha-D-maltoside
-
-
-
?
2.4.1.4
sucrose + maltose
the enzyme also produces (+)-catechin maltooligosaccharides
719376
Deinococcus geothermalis DSM 11300
D-fructose + (+)-catechin-3'-O-alpha-D-maltoside
-
-
-
?
2.4.1.4
sucrose + [(1->4)-alpha-D-glucosyl]n
-
719376
Deinococcus geothermalis
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
2.4.1.4
sucrose + [(1->4)-alpha-D-glucosyl]n
a phenolic aglycone compound can also act an acceptor molecule of ASase transglycosylation activity
719376
Deinococcus geothermalis
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
2.4.1.4
sucrose + [(1->4)-alpha-D-glucosyl]n
-
719376
Deinococcus geothermalis DSM 11300
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
2.4.1.4
sucrose + [(1->4)-alpha-D-glucosyl]n
a phenolic aglycone compound can also act an acceptor molecule of ASase transglycosylation activity
719376
Deinococcus geothermalis DSM 11300
D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.4
45
-
assay at
Deinococcus geothermalis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.4
7
-
assay at
Deinococcus geothermalis