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Literature summary extracted from

  • Choi, J.; Gedi, V.; Pham, C.; Ryu, K.; Lee, H.; Kim, G.; Yoon, M.
    Site-directed mutagenesis of catalytic and regulatory subunits of Mycobacterium tuberculosis acetohydroxyacid synthase (2010), Enzyme Microb. Technol., 46, 304-308.
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.6 F147A 4fold decrease in vmax value, strong resistance to sulfonylurea inhibitors Mycobacterium tuberculosis
2.2.1.6 F147R 2.5fold decrease in vmax value, strong resistance to sulfonylurea inhibitors Mycobacterium tuberculosis
2.2.1.6 L141A 5fold decrease in vmax value Mycobacterium tuberculosis
2.2.1.6 W561R 30fold decrease in vmax value, strong resistance to sulfonylurea inhibitors Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 L-isoleucine
-
 Mycobacterium tuberculosis
2.2.1.6 L-leucine
-
 Mycobacterium tuberculosis
2.2.1.6 L-valine
-
 Mycobacterium tuberculosis
2.2.1.6 Londax
-
 Mycobacterium tuberculosis
2.2.1.6 NC-311
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 1.08
-
 pyruvate mutant F147R, pH 7.5, 37°C Mycobacterium tuberculosis
2.2.1.6 1.18
-
 pyruvate wild-type, pH 7.5, 37°C Mycobacterium tuberculosis
2.2.1.6 1.89
-
 pyruvate mutant L141A, pH 7.5, 37°C Mycobacterium tuberculosis
2.2.1.6 2.49
-
 pyruvate wild-type, pH 7.5, 37°C Mycobacterium tuberculosis
2.2.1.6 3.35
-
 pyruvate mutant L89A, pH 7.5, 37°C Mycobacterium tuberculosis
2.2.1.6 3.89
-
 pyruvate mutant F147A, pH 7.5, 37°C Mycobacterium tuberculosis
2.2.1.6 4.94
-
 pyruvate mutant W516R, pH 7.5, 37°C Mycobacterium tuberculosis
2.2.1.6 5.8
-
 pyruvate mutant R101A, pH 7.5, 37°C Mycobacterium tuberculosis
2.2.1.6 8.9
-
 pyruvate mutant S27A, pH 7.5, 37°C Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.6 20000
-
 x * 20000, SDS-PAGE Mycobacterium tuberculosis
2.2.1.6 68000
-
 x * 68000, SDS-PAGE Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Mycobacterium tuberculosis
-
 regulatory subunit
-
2.2.1.6 Mycobacterium tuberculosis P9WG41 catalytic subunit
-
2.2.1.6 Mycobacterium tuberculosis H37Rv P9WG41 catalytic subunit
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.6 0.17
-
 isolated catalytic subunit, pH 7.5, 37°C Mycobacterium tuberculosis
2.2.1.6 1.13
-
 pH 7.5, 37°C Mycobacterium tuberculosis
2.2.1.6 1.13
-
 holoenzyme, pH 7.5, 37°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 2 pyruvate
-
 Mycobacterium tuberculosis 2-acetolactate + CO2
-
 ?
2.2.1.6 2 pyruvate
-
 Mycobacterium tuberculosis H37Rv 2-acetolactate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.6 ? x * 20000, SDS-PAGE Mycobacterium tuberculosis
2.2.1.6 ? x * 68000, SDS-PAGE Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 thiamine diphosphate Km value 0.027 mM Mycobacterium tuberculosis
2.2.1.6 thiamine diphosphate Km value 0.036 mM Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.2.1.6 0.00064
-
 wild-type, pH 7.5, 37°C Mycobacterium tuberculosis Londax
2.2.1.6 0.0014
-
 R101A, pH 7.5, 37°C Mycobacterium tuberculosis L-leucine
2.2.1.6 0.0016
-
 wild-type, pH 7.5, 37°C Mycobacterium tuberculosis L-leucine
2.2.1.6 0.004
-
 R101A, pH 7.5, 37°C Mycobacterium tuberculosis L-valine
2.2.1.6 0.005
-
 wild-type, pH 7.5, 37°C Mycobacterium tuberculosis L-valine
2.2.1.6 0.0075
-
 mutant S27A, pH 7.5, 37°C Mycobacterium tuberculosis L-valine
2.2.1.6 0.011
-
 mutant L141A, pH 7.5, 37°C Mycobacterium tuberculosis NC-311
2.2.1.6 0.012
-
 wild-type, pH 7.5, 37°C Mycobacterium tuberculosis L-isoleucine
2.2.1.6 0.012
-
 L89A, pH 7.5, 37°C Mycobacterium tuberculosis L-valine
2.2.1.6 0.021
-
 mutant L141A, pH 7.5, 37°C Mycobacterium tuberculosis Londax
2.2.1.6 0.04
-
 R101A, pH 7.5, 37°C Mycobacterium tuberculosis L-isoleucine
2.2.1.6 0.048
-
 wild-type, pH 7.5, 37°C Mycobacterium tuberculosis NC-311
2.2.1.6 0.062
-
 mutant S27A, pH 7.5, 37°C Mycobacterium tuberculosis L-isoleucine
2.2.1.6 0.32
-
 L89A, pH 7.5, 37°C Mycobacterium tuberculosis L-isoleucine