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Literature summary extracted from

  • Lipps, G.; Röther, S.; Hart, C.; Krauss, G.
    A novel type of replicative enzyme harbouring ATPase, primase and DNA polymerase activity (2003), EMBO J., 22, 2516-2525.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.102 expression in Escherichia coli as a His-tagged protein Sulfolobus islandicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.102 D111A the mutant protein is deficient and DNA polymerase activity and primase activity, ATPase activity is unaffected Sulfolobus islandicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.102 Sulfolobus islandicus Q54324 plasmid pRN1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.102
-
 Sulfolobus islandicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.102 ATP + 7 dNTP formation of a ribonucleotide primer when ORF904 is incubated with ribonucleotides and single-stranded M13 DNA. Effcient dNTP incorporation is strongly dependent on the presence of ATP. The non-hydrolysable analogues beta,gamma-imino-ATP and beta,gamma-methylene-ATP are active in stimulation of primer formation. alpha,beta-methylene-ATP does not enhance primer formation. The stimulatory effect is also seen for GTP and UTP but not for ADP or dATP. The majority of the primers have a length of eight nucleotides. Protein ORF904 shows ATPase, DNA polymerase and primase activity Sulfolobus islandicus A(pdN)7 + 7 diphosphate
-
 ?
2.7.7.102 NTP + 7 dNTP formation of a ribonucleotide primer when ORF904 is incubated with ribonucleotides and single-stranded M13 DNA. Effcient dNTP incorporation is strongly dependent on the presence of ATP. The non-hydrolysable analogues beta,gamma-imino-ATP and beta,gamma-methylene-ATP are active in stimulation of primer formation. alpha,beta-methylene-ATP does not enhance primer formation. The stimulatory effect is also seen for GTP and UTP but not for ADP or dATP. The majority of the primers have a length of eight nucleotides. Protein ORF904 shows ATPase, DNA polymerase and primase activity Sulfolobus islandicus N(pdN)7 + 7 diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.102 protein ORF904
-
 Sulfolobus islandicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.102 50 60
-
 Sulfolobus islandicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.102 7.5
-
 assay at Sulfolobus islandicus