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Literature summary extracted from

  • Badenhorst, C.P.; Jooste, M.; van Dijk, A.A.
    Enzymatic characterization and elucidation of the catalytic mechanism of a recombinant bovine glycine N-acyltransferase (2012), Drug Metab. Dispos., 40, 346-352.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.13
-
 Bos taurus
2.3.1.71
-
 Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.13 E226Q about 3fold increase in Km value for lgycine Bos taurus
2.3.1.71 E226Q about 3fold increase in Km value for lgycine Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.13 0.0016
-
 glycine wild-type, pH 8.0, 30°C Bos taurus
2.3.1.13 0.002
-
 glycine recombinant wild-type, pH 8.0, 30°C Bos taurus
2.3.1.13 0.007
-
 glycine mutant E226Q, pH 8.0, 30°C Bos taurus
2.3.1.13 0.016
-
 benzoyl-CoA wild-type, pH 8.0, 30°C Bos taurus
2.3.1.13 0.016
-
 benzoyl-CoA recombinant wild-type, pH 8.0, 30°C Bos taurus
2.3.1.13 0.018
-
 benzoyl-CoA mutant E226Q, pH 8.0, 30°C Bos taurus
2.3.1.71 0.0016
-
 glycine wild-type, pH 8.0, 30°C Bos taurus
2.3.1.71 0.002
-
 glycine recombinant wild-type, pH 8.0, 30°C Bos taurus
2.3.1.71 0.007
-
 glycine mutant E226Q, pH 8.0, 30°C Bos taurus
2.3.1.71 0.016
-
 benzoyl-CoA wild-type, pH 8.0, 30°C Bos taurus
2.3.1.71 0.016
-
 benzoyl-CoA recombinant wild-type, pH 8.0, 30°C Bos taurus
2.3.1.71 0.018
-
 benzoyl-CoA mutant E226Q, pH 8.0, 30°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.13 mitochondrion
-
 Bos taurus 5739
-
2.3.1.71 mitochondrion
-
 Bos taurus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.13 Bos taurus Q2KIR7
-
-
2.3.1.71 Bos taurus Q2KIR7
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.13 acyl-CoA + glycine = CoA + N-acylglycine residue Glu226 functions to deprotonate glycine, facilitating nucleophilic attack on the acyl-CoA substrate Bos taurus
a
2.3.1.71 benzoyl-CoA + glycine = CoA + hippurate residue Glu226 functions to deprotonate glycine, facilitating nucleophilic attack on the acyl-CoA substrate Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.13 liver
-
 Bos taurus
-
2.3.1.71 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.13 benzoyl-CoA + glycine
-
 Bos taurus CoA + N-benzoylglycine
-
 ?
2.3.1.71 benzoyl-CoA + glycine
-
 Bos taurus CoA + N-benzoylglycine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.13 additional information
-
 for mutant E226Q, activity increases significantly when raising the pH above 8.0, while for wild-type, activity remains more or less stable up to pH 9.0 Bos taurus
2.3.1.13 7.5 8.5 wild-type Bos taurus
2.3.1.71 additional information
-
 for mutant E226Q, activity increases significantly when raising the pH above 8.0, while for wild-type, activity remains more or less stable up to pH 9.0 Bos taurus
2.3.1.71 7.5 8.5 wild-type Bos taurus