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Literature summary extracted from

  • Yin, Y.; Zhou, N.Y.
    Characterization of MnpC, a hydroquinone dioxygenase likely involved in the meta-nitrophenol degradation by Cupriavidus necator JMP134 (2010), Curr. Microbiol., 61, 471-476.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.66 expression in Escherichia coli Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.66 0.255
-
 hydroquinone pH 7.4, temperature not specified in the publication Cupriavidus necator

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.66 Iron preincubation in presence of 0.1 mM Fe2+ and 1 mM Mn2+ increases activity by 10fold Cupriavidus necator
1.13.11.66 Manganese preincubation in presence of 0.1 mM Fe2+ and 1 mM Mn2+ increases activity by 10fold Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.66 34000
-
 x * 34000, SDS-PAGE Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.66 Cupriavidus necator
-
-
-
1.13.11.66 Cupriavidus necator JMP 134-1
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.66 3.1
-
 pH 7.4, temperature not specified in the publication Cupriavidus necator

Storage Stability

EC Number Storage Stability Organism
1.13.11.66 -20°C, 50% glycerol, 48% residual activity after 30 days Cupriavidus necator
1.13.11.66 -70°C, 50% glycerol, 77% residual activity after 30 days Cupriavidus necator
1.13.11.66 fairly stable for several hours on ice and retains approximately 80% of activity after 72 h Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.66 hydroquinone + O2
-
 Cupriavidus necator 4-hydroxymuconic semialdehyde
-
 ?
1.13.11.66 hydroquinone + O2
-
 Cupriavidus necator JMP 134-1 4-hydroxymuconic semialdehyde
-
 ?
1.13.11.66 additional information no substrate: hydroxyquinol, catechol, 2-aminophenol,4-aminophenol, protocatechuate, and gentisate Cupriavidus necator ?
-
 ?
1.13.11.66 additional information no substrate: hydroxyquinol, catechol, 2-aminophenol,4-aminophenol, protocatechuate, and gentisate Cupriavidus necator JMP 134-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.66 ? x * 34000, SDS-PAGE Cupriavidus necator

Synonyms

EC Number Synonyms Comment Organism
1.13.11.66 mnpC gen name Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.66 7.4 8
-
 Cupriavidus necator

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.66 7
-
 20% of maximum activity Cupriavidus necator