EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.283 | co-expressed with molecular chaperone GroES and GroEL in Escherichia coli BL21(DE3) cells | Streptomyces sp. |
2.4.1.283 | expressed as a His-tagged fusion protein in Escherichia coli | Streptomyces fradiae |
2.4.1.285 | expressed as a His-tagged fusion protein in Escherichia coli | Streptomyces fradiae |
2.4.1.285 | expressed in Escherichia coli BL21(DE3) cells | Streptomyces sp. |
3.5.1.112 | expressed as a His-tagged fusion protein in Escherichia coli | Streptomyces fradiae |
3.5.1.113 | expressed as a His-tagged fusion protein in Escherichia coli | Streptomyces fradiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.285 | Ca2+ | about 55% residual activity in the presence of 2 mM | Streptomyces sp. | |
2.4.1.285 | Co2+ | about 90% residual activity in the presence of 2 mM | Streptomyces sp. | |
2.4.1.285 | Cu2+ | inhibits activity | Streptomyces fradiae | |
2.4.1.285 | Cu2+ | complete inhibition in the presence of 2 mM | Streptomyces sp. | |
2.4.1.285 | EDTA | about 20% residual activity in the presence of 10 mM | Streptomyces sp. | |
2.4.1.285 | Ni2+ | about 80% residual activity in the presence of 2 mM | Streptomyces sp. | |
2.4.1.285 | Zn2+ | inhibits activity | Streptomyces fradiae | |
2.4.1.285 | Zn2+ | complete inhibition in the presence of 2 mM | Streptomyces sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.285 | Mg2+ | - |
Streptomyces fradiae | |
2.4.1.285 | Mg2+ | 100% activity in the presence of 2 mM | Streptomyces sp. | |
2.4.1.285 | Mn2+ | - |
Streptomyces fradiae | |
2.4.1.285 | Mn2+ | 105% activity in the presence of 2 mM Mn2+ compared to Mg2+ | Streptomyces sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.283 | Streptomyces fradiae | - |
- |
- |
2.4.1.283 | Streptomyces sp. | - |
- |
- |
2.4.1.285 | Streptomyces fradiae | - |
- |
- |
2.4.1.285 | Streptomyces sp. | - |
- |
- |
3.5.1.112 | Streptomyces fradiae | - |
- |
- |
3.5.1.113 | Streptomyces fradiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.283 | a poor amount is isolated using Ni-NTA chromatography. The cell-free extract of Escherichia coli expressing NeoD is used for enzymatic assays | Streptomyces fradiae |
2.4.1.285 | DEAE Sepharose column chromatography, Superdex Hi-Load 200 gel filtration, and Mono Q column chromatography | Streptomyces sp. |
2.4.1.285 | purified to homogeneity by DEAE Sepharose column chromatography, gel filtration, and MonoQ column chromatography | Streptomyces fradiae |
3.5.1.112 | purified with TALON cobalt affinity resin | Streptomyces fradiae |
3.5.1.113 | purified with TALON cobalt affinity resin | Streptomyces fradiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.283 | additional information | other possible glycosyl donor, UDP-glucose, and other possible glycosyl acceptors, paromamine, neamine, and ribostamycin are not accepted | Streptomyces fradiae | ? | - |
? | |
2.4.1.283 | additional information | the enzyme is unable to catalyze the glycosylation of ribostamycin | Streptomyces sp. | ? | - |
? | |
2.4.1.283 | UDP-N-acetyl-alpha-D-glucosamine + 2-deoxystreptamine | - |
Streptomyces fradiae | UDP + 2'-N-acetylparomamine | - |
? | |
2.4.1.283 | UDP-N-acetyl-alpha-D-glucosamine + 2-deoxystreptamine | about 80% of 2-deoxystreptamine is conversed | Streptomyces sp. | UDP + 2'-N-acetylparomamine | - |
? | |
2.4.1.285 | UDP-N-acetyl-alpha-D-glucosamine + ribostamycin | - |
Streptomyces sp. | UDP + 2'''-acetyl-6'''-hydroxyneomycin C | - |
? | |
2.4.1.285 | UDP-N-acetyl-alpha-D-glucosamine + ribostamycin | - |
Streptomyces fradiae | UDP + 2'''-acetyl-6'''-hydroxyneomycin C | - |
? | |
3.5.1.112 | N-acetylparomamine + H2O | - |
Streptomyces fradiae | paromamine + acetate | - |
? | |
3.5.1.113 | 2'''-N-acetyl-6'''-deamino-6'''-hydroxyneomycin C + H2O | - |
Streptomyces fradiae | 6'''-deamino-6'''-hydroxyneomycin C | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.283 | neoD | - |
Streptomyces sp. |
2.4.1.283 | neoD | - |
Streptomyces fradiae |
2.4.1.285 | Neo15 | - |
Streptomyces sp. |
2.4.1.285 | neoK | - |
Streptomyces sp. |
2.4.1.285 | neoK | - |
Streptomyces fradiae |
3.5.1.112 | neoL | - |
Streptomyces fradiae |
3.5.1.113 | neoL | - |
Streptomyces fradiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.283 | 28 | - |
assay at | Streptomyces fradiae |
2.4.1.285 | 28 | - |
assay at | Streptomyces fradiae |
3.5.1.112 | 28 | - |
assay at | Streptomyces fradiae |
3.5.1.113 | 28 | - |
assay at | Streptomyces fradiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.283 | 7 | - |
assay at | Streptomyces fradiae |
2.4.1.285 | 7 | - |
assay at | Streptomyces fradiae |
3.5.1.112 | 7 | - |
assay at | Streptomyces fradiae |
3.5.1.113 | 7 | - |
assay at | Streptomyces fradiae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.1.285 | metabolism | NeoK is a glycosyltransferase that catalyzes the retaining glycosylation of ribostamycin with UDP-GlcNAc as a sugar donor | Streptomyces fradiae |