Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Huang, F.; Spiteller, D.; Koorbanally, N.A.; Li, Y.; Llewellyn, N.M.; Spencer, J.B.
    Elaboration of neosamine rings in the biosynthesis of neomycin and butirosin (2007), ChemBioChem, 8, 283-288.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.43 expression in Escherichia coli Streptomyces fradiae
1.1.3.44 expression in Escherichia coli Streptomyces fradiae
2.6.1.93 expressed in Escherichia coli BL21(DE3) cells Niallia circulans
2.6.1.93 expressed in Escherichia coli BL21(DE3) cells Streptomyces fradiae
2.6.1.95 expressed in Escherichia coli BL21(DE3) cells Streptomyces fradiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.43 60034
-
 x * 60034, LC-ESI-MS Streptomyces fradiae
1.1.3.44 60034
-
 x * 60034, LC-ESI-MS Streptomyces fradiae
2.6.1.93 48060
-
 Neo-18, calculated from amino acid sequence Streptomyces fradiae
2.6.1.93 48060
-
 Neo-18, electrospray ionization mass spectrometry Streptomyces fradiae
2.6.1.93 52070
-
 BtrB, calculated from amino acid sequence Niallia circulans
2.6.1.93 52070
-
 BtrB, electrospray ionization mass spectrometry Niallia circulans
2.6.1.95 48060
-
 Neo-18, calculated from amino acid sequence Streptomyces fradiae
2.6.1.95 48060
-
 Neo-18, electrospray ionization mass spectrometry Streptomyces fradiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.43 Streptomyces fradiae Q53U15
-
-
1.1.3.43 Streptomyces fradiae NCIMB 8233 Q53U15
-
-
1.1.3.44 Streptomyces fradiae Q53U15
-
-
1.1.3.44 Streptomyces fradiae NCIMB 8233 Q53U15
-
-
2.6.1.93 Niallia circulans
-
-
-
2.6.1.93 Niallia circulans B3312
-
-
-
2.6.1.93 Streptomyces fradiae
-
-
-
2.6.1.93 Streptomyces fradiae NCIMB 8233
-
-
-
2.6.1.95 Streptomyces fradiae
-
-
-
2.6.1.95 Streptomyces fradiae NCIMB 8233
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.93 Ni2+-NTA resin column chromatography Niallia circulans
2.6.1.93 Ni2+-NTA resin column chromatography Streptomyces fradiae
2.6.1.95 Ni2+-NTA resin column chromatography Streptomyces fradiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.43 additional information enzyme also catalyzes reaction of EC 1.1.3.44, 6''-hydroxyneomycin C oxidase Streptomyces fradiae ?
-
 ?
1.1.3.43 additional information enzyme also catalyzes reaction of EC 1.1.3.44, 6''-hydroxyneomycin C oxidase Streptomyces fradiae NCIMB 8233 ?
-
 ?
1.1.3.43 paromamine + O2 oxygen-dependent mechanism for the regeneration of the FAD cofactor Streptomyces fradiae 6'-dehydro-paromamine + H2O2
-
 ?
1.1.3.43 paromamine + O2 oxygen-dependent mechanism for the regeneration of the FAD cofactor Streptomyces fradiae NCIMB 8233 6'-dehydro-paromamine + H2O2
-
 ?
1.1.3.44 6'''-deamino-6'''-hydroxyneomycin C + O2 oxygen-dependent mechanism for the regeneration of the FAD cofactor Streptomyces fradiae 6'''-deamino-6'''-oxoneomycin C + H2O2
-
 ?
1.1.3.44 6'''-deamino-6'''-hydroxyneomycin C + O2 oxygen-dependent mechanism for the regeneration of the FAD cofactor Streptomyces fradiae NCIMB 8233 6'''-deamino-6'''-oxoneomycin C + H2O2
-
 ?
1.1.3.44 additional information enzyme also catalyzes reaction of EC 1.1.3.43, paromamine 6'-oxidase Streptomyces fradiae ?
-
 ?
1.1.3.44 additional information enzyme also catalyzes reaction of EC 1.1.3.43, paromamine 6'-oxidase Streptomyces fradiae NCIMB 8233 ?
-
 ?
2.6.1.93 6'-dehydroparomamine + L-glutamate
-
 Niallia circulans neamine + 2-oxoglutarate
-
 ?
2.6.1.93 6'-dehydroparomamine + L-glutamate
-
 Streptomyces fradiae neamine + 2-oxoglutarate
-
 ?
2.6.1.93 6'-dehydroparomamine + L-glutamate
-
 Streptomyces fradiae NCIMB 8233 neamine + 2-oxoglutarate
-
 ?
2.6.1.93 6'-dehydroparomamine + L-glutamate
-
 Niallia circulans B3312 neamine + 2-oxoglutarate
-
 ?
2.6.1.95 neomycin + 2-oxoglutarate
-
 Streptomyces fradiae 6'''-deamino-6'''-dehydro-6'''-oxoneomycin + L-glutamate
-
 ?
2.6.1.95 neomycin + 2-oxoglutarate
-
 Streptomyces fradiae NCIMB 8233 6'''-deamino-6'''-dehydro-6'''-oxoneomycin + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.43 ? x * 60034, LC-ESI-MS Streptomyces fradiae
1.1.3.44 ? x * 60034, LC-ESI-MS Streptomyces fradiae

Synonyms

EC Number Synonyms Comment Organism
1.1.3.44 Neo-11
-
 Streptomyces fradiae
2.6.1.93 6'-oxoglucosaminyl:L-glutamate aminotransferase
-
 Niallia circulans
2.6.1.93 6'-oxoglucosaminyl:L-glutamate aminotransferase
-
 Streptomyces fradiae
2.6.1.93 btrB
-
 Niallia circulans
2.6.1.93 Neo-18
-
 Streptomyces fradiae
2.6.1.95 6'-oxoglucosaminyl:L-glutamate aminotransferase
-
 Streptomyces fradiae
2.6.1.95 Neo-18
-
 Streptomyces fradiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.43 30
-
 assay at Streptomyces fradiae
1.1.3.44 30
-
 assay at Streptomyces fradiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.43 8
-
 assay at Streptomyces fradiae
1.1.3.44 8
-
 assay at Streptomyces fradiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.43 FAD
-
 Streptomyces fradiae
1.1.3.44 FAD
-
 Streptomyces fradiae

General Information

EC Number General Information Comment Organism
1.1.3.43 physiological function joint activity of enzyme and 6'-oxoglucosaminyl:L-glutamate aminotransferase Neo-18 is responsible for the conversion of paromamine to neaminein the biosynthetic pathway of neomycin through a mechanism of FAD-dependent dehydrogenation followed by a pyridoxal-5’-phosphate-mediated transamination Streptomyces fradiae
1.1.3.44 physiological function joint activity of enzyme and 6'-oxoglucosaminyl:L-glutamate aminotransferase Neo-18 is responsible for the conversion of paromamine to neaminein the biosynthetic pathway of neomycin through a mechanism of FAD-dependent dehydrogenation followed by a pyridoxal-5'-phosphate-mediated transamination Streptomyces fradiae
2.6.1.93 metabolism BtrB is involved in neamine biosynthesis Niallia circulans
2.6.1.93 metabolism Neo-18 is involved in neamine biosynthesis Streptomyces fradiae