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Literature summary extracted from

  • Liu, X.; Wang, L.; Steffan, N.; Yin, W.; Li, S.
    Ergot alkaloid biosynthesis in Aspergillus fumigatus: FgaAT catalyses the acetylation of fumigaclavine B (2009), ChemBioChem, 10, 2325-2328.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.205 gene AFUA_2G18020, DNA and amino acid seqquence determmination and analysis, sequence comparison, expression of His6-tagged FgaAT in Escherichia coli Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.205 additional information
-
 additional information the FgaAT reaction apparently follows Michaelis-Menten kinetics Aspergillus fumigatus
2.3.1.205 0.122
-
 acetyl-CoA pH not specified in the publication, 37°C, recombinant His6-tagged enzyme Aspergillus fumigatus
2.3.1.205 0.147
-
 fumigaclavine B pH not specified in the publication, 37°C, recombinant His6-tagged enzyme Aspergillus fumigatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.205 Ca2+ 22% activation at 5 mM Aspergillus fumigatus
2.3.1.205 additional information no effects by Na+ and K+ at 5 mM, the acetyl-transfer reaction catalysed by FgaAT is very likely independent of the presence of metal ions Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.205 55000
-
 1 * 55000, about, wild-type enzyme, sequence calculation, 1 * 56700, about, His6-tagged enzyme, sequence calculation, 1 * 57000, recombinant His6-tagged enzyme, SDS-PAGE Aspergillus fumigatus
2.3.1.205 56700
-
 1 * 55000, about, wild-type enzyme, sequence calculation, 1 * 56700, about, His6-tagged enzyme, sequence calculation, 1 * 57000, recombinant His6-tagged enzyme, SDS-PAGE Aspergillus fumigatus
2.3.1.205 57000
-
 1 * 55000, about, wild-type enzyme, sequence calculation, 1 * 56700, about, His6-tagged enzyme, sequence calculation, 1 * 57000, recombinant His6-tagged enzyme, SDS-PAGE Aspergillus fumigatus
2.3.1.205 60800
-
 recombinant His6-tagged enzyme, gel filtration Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.205 acetyl-CoA + fumigaclavine B Aspergillus fumigatus
-
 CoA + fumigaclavine A
-
 ?
2.3.1.205 acetyl-CoA + fumigaclavine B Aspergillus fumigatus Af293
-
 CoA + fumigaclavine A
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.205 Aspergillus fumigatus Q4WZ64 gene AFUA_2G18020
-
2.3.1.205 Aspergillus fumigatus Af293 Q4WZ64 gene AFUA_2G18020
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.205 acetyl-CoA + fumigaclavine B
-
 Aspergillus fumigatus CoA + fumigaclavine A
-
 ?
2.3.1.205 acetyl-CoA + fumigaclavine B
-
 Aspergillus fumigatus Af293 CoA + fumigaclavine A
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.205 monomer 1 * 55000, about, wild-type enzyme, sequence calculation, 1 * 56700, about, His6-tagged enzyme, sequence calculation, 1 * 57000, recombinant His6-tagged enzyme, SDS-PAGE Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.205 FgaAT
-
 Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.205 0.6
-
 acetyl-CoA pH not specified in the publication, 37°C, recombinant His6-tagged enzyme Aspergillus fumigatus
2.3.1.205 0.6
-
 fumigaclavine B pH not specified in the publication, 37°C, recombinant His6-tagged enzyme Aspergillus fumigatus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.205 acetyl-CoA
-
 Aspergillus fumigatus

General Information

EC Number General Information Comment Organism
2.3.1.205 physiological function FgaAT catalyses the acetylation of fumigaclavine B, the conversion of fumigaclavine B to A, which belong to the ergot alkaloids of the clavine-type Aspergillus fumigatus