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Literature summary extracted from

  • Sidoruk, K.; Pokrovsky, V.; Borisova, A.; Omeljanuk, N.; Aleksandrova, S.; Pokrovskaya, M.; Gladilina, J.; Bogush, V.; Sokolov, N.
    Creation of a producent, optimization of expression, and purifi cation of recombinant Yersinia Pseudotuberculosis L-asparaginase (2011), Bull. Exp. Biol. Med., 152, 219-223.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 gene ansB, expression in Escherichia coli strain BL21 (DE3), method evaluation, overview Yersinia pseudotuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 L-asparagine + H2O Yersinia pseudotuberculosis
-
 L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O Yersinia pseudotuberculosis IP32953
-
 L-aspartate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Yersinia pseudotuberculosis Q66CJ2 gene ansB
-
3.5.1.1 Yersinia pseudotuberculosis IP32953 Q66CJ2 gene ansB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 recombinant YpA from Escherichia coli strain BL21 (DE3) by two different steps of anion exchange chromatography Yersinia pseudotuberculosis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.1 cell culture optimal conditions of induction are: inductor concentration of 0.1-0.2%, its addition during the middle of the logphase at 1.45-2.3 OU600, and culturing temperature of 28°C. The optimal duration of culturing after induction is 7-16 h Yersinia pseudotuberculosis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 62.7
-
 pH 8.0, 37°C, purified enzyme Yersinia pseudotuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Yersinia pseudotuberculosis L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O
-
 Yersinia pseudotuberculosis IP32953 L-aspartate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 L-asparaginase II
-
 Yersinia pseudotuberculosis
3.5.1.1 Ypa
-
 Yersinia pseudotuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 60
-
-
 Yersinia pseudotuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 8
-
-
 Yersinia pseudotuberculosis

Expression

EC Number Organism Comment Expression
3.5.1.1 Yersinia pseudotuberculosis optimal conditions of induction are: inductor concentration of 0.1-0.2%, its addition during the middle of the logphase at 1.45-2.3 OU600, and culturing temperature of 28°C. The optimal duration of culturing after induction is 7-16 h up